PMID- 32502464
OWN - NLM
STAT- MEDLINE
DCOM- 20210602
LR  - 20210602
IS  - 2213-6711 (Electronic)
IS  - 2213-6711 (Linking)
VI  - 15
IP  - 1
DP  - 2020 Jul 14
TI  - Cryopreserved Human Oocytes and Cord Blood Cells Can Produce Somatic Cell Nuclear 
      Transfer-Derived Pluripotent Stem Cells with a Homozygous HLA Type.
PG  - 171-184
LID - S2213-6711(20)30177-6 [pii]
LID - 10.1016/j.stemcr.2020.05.005 [doi]
AB  - Human pluripotent stem cells (PSCs) through somatic cell nuclear transfer (SCNT) 
      may be an important source for regenerative medicine. The low derivation 
      efficiency of stem cells and the accessibility of human oocytes are the main 
      obstacles to their application. We previously reported that the efficiency of 
      SCNT was increased by overexpression of H3K9me3 demethylase. Here, we applied a 
      modified derivation method to the PSC line and first obtained human SCNT-PSC 
      lines derived from both donated cryopreserved oocytes and cord blood cells with a 
      homozygous human leukocyte antigen (HLA) type. The SCNT-PSCs have very similar 
      characteristics with embryonic stem cells (ESCs) and additionally have shown 
      immunocompatibility in an in vitro and in vivo humanized mouse with a matching 
      HLA type. Our study demonstrates that SCNT technology using donated cryopreserved 
      oocytes and cord blood cells with a known HLA type provides a promising method 
      for establishing a human HLA-matched SCNT-PSC bank for regenerative medicine.
CI  - Copyright (c) 2020 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Lee, Jeoung Eun
AU  - Lee JE
AD  - CHA Advanced Research Institute, CHA University, Seongnam, Gyunggi-do 13488, 
      Korea.
FAU - Lee, Ji Yoon
AU  - Lee JY
AD  - CHA Advanced Research Institute, CHA University, Seongnam, Gyunggi-do 13488, 
      Korea.
FAU - Park, Chang-Hwan
AU  - Park CH
AD  - Graduated School of Biomedical Science and Engineering, Hanyang University, Seoul 
      04763, Korea.
FAU - Eum, Jin Hee
AU  - Eum JH
AD  - Fertility Center of CHA Gangnam Medical Center, CHA University, Seoul 06135, 
      Korea.
FAU - Jung, Soo Kyung
AU  - Jung SK
AD  - CHA Advanced Research Institute, CHA University, Seongnam, Gyunggi-do 13488, 
      Korea.
FAU - Han, A-Reum
AU  - Han AR
AD  - Department of Biomedical Science, CHA University, Seongnam, Gyunggi-do 13488, 
      Korea.
FAU - Seol, Dong-Won
AU  - Seol DW
AD  - Department of Biomedical Science, CHA University, Seongnam, Gyunggi-do 13488, 
      Korea.
FAU - Lee, Jin Saem
AU  - Lee JS
AD  - Graduated School of Biomedical Science and Engineering, Hanyang University, Seoul 
      04763, Korea.
FAU - Shin, Hyun Soo
AU  - Shin HS
AD  - Department of Radiation Oncology, CHA Bundang Medical Center, CHA University, 
      Seongnam 13496, Korea.
FAU - Im, Jung Ho
AU  - Im JH
AD  - Department of Radiation Oncology, CHA Bundang Medical Center, CHA University, 
      Seongnam 13496, Korea.
FAU - Chun, Taehoon
AU  - Chun T
AD  - Department of Biotechnology, College of Life and Biotechnology, Korea University, 
      Seoul 02841, Korea.
FAU - Ha, Kyungsoo
AU  - Ha K
AD  - New Drug Development Center, Osong Medical Innovation Foundation, Osong 28160, 
      Korea.
FAU - Heo, Deok Rim
AU  - Heo DR
AD  - New Drug Development Center, Osong Medical Innovation Foundation, Osong 28160, 
      Korea.
FAU - Yoon, Tae Ki
AU  - Yoon TK
AD  - Department of Biomedical Science, CHA University, Seongnam, Gyunggi-do 13488, 
      Korea.
FAU - Lee, Dong Ryul
AU  - Lee DR
AD  - CHA Advanced Research Institute, CHA University, Seongnam, Gyunggi-do 13488, 
      Korea; Department of Biomedical Science, CHA University, Seongnam, Gyunggi-do 
      13488, Korea. Electronic address: drleedr@cha.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200604
PL  - United States
TA  - Stem Cell Reports
JT  - Stem cell reports
JID - 101611300
RN  - 0 (Biomarkers)
RN  - 0 (HLA Antigens)
SB  - IM
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Cell Differentiation
MH  - Cell Line
MH  - Cell Lineage
MH  - *Cryopreservation
MH  - Fetal Blood/*cytology
MH  - HLA Antigens/*metabolism
MH  - Homozygote
MH  - Humans
MH  - Mice
MH  - Models, Animal
MH  - *Nuclear Transfer Techniques
MH  - Oocytes/*cytology
MH  - Osteoblasts/metabolism
MH  - Pluripotent Stem Cells/*cytology
PMC - PMC7363744
OTO - NOTNLM
OT  - frozen cord blood cells
OT  - frozen oocytes
OT  - homozygous HLA
OT  - pluripotent stem cells
OT  - somatic cell nuclear transfer
EDAT- 2020/06/06 06:00
MHDA- 2021/06/03 06:00
PMCR- 2020/06/04
CRDT- 2020/06/06 06:00
PHST- 2020/04/24 00:00 [received]
PHST- 2020/05/05 00:00 [revised]
PHST- 2020/05/05 00:00 [accepted]
PHST- 2020/06/06 06:00 [pubmed]
PHST- 2021/06/03 06:00 [medline]
PHST- 2020/06/06 06:00 [entrez]
PHST- 2020/06/04 00:00 [pmc-release]
AID - S2213-6711(20)30177-6 [pii]
AID - 10.1016/j.stemcr.2020.05.005 [doi]
PST - ppublish
SO  - Stem Cell Reports. 2020 Jul 14;15(1):171-184. doi: 10.1016/j.stemcr.2020.05.005. 
      Epub 2020 Jun 4.