PMID- 32502571
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20210514
IS  - 1873-7544 (Electronic)
IS  - 0306-4522 (Linking)
VI  - 441
DP  - 2020 Aug 10
TI  - Gravitational Transitions Increase Posterior Cerebral Perfusion and Systemic 
      Oxidative-nitrosative Stress: Implications for Neurovascular Unit Integrity.
PG  - 142-160
LID - S0306-4522(20)30350-X [pii]
LID - 10.1016/j.neuroscience.2020.05.048 [doi]
AB  - The present study examined if repeated bouts of micro- and hypergravity during 
      parabolic flight (PF) alter structural integrity of the neurovascular unit (NVU) 
      subsequent to free radical-mediated changes in regional cerebral perfusion. Six 
      participants (5male symbol, 1female symbol) aged 29 +/- 11 years were examined before, during and after a 
      3 h PF and compared to six sex and age-matched (27 +/- 6 years) normogravity 
      controls. Blood flow was measured in the anterior (middle cerebral artery, MCA; 
      internal carotid artery, ICA) and posterior (vertebral artery, VA) circulation 
      (duplex ultrasound) in-flight over the course of 15 parabolas. Venous blood was 
      assayed for free radicals (electron paramagnetic resonance spectroscopy), nitric 
      oxide (NO, ozone-based chemiluminescence) and NVU integrity 
      (chemiluminescence/ELISA) in normogravity before and after exposure to 31 
      parabolas. While MCA velocity did not change (P > 0.05), a selective increase in 
      VA flow was observed during the most marked gravitational transition from micro- 
      to hypergravity (P < 0.05). Increased oxidative-nitrosative stress defined by a 
      free radical-mediated reduction in NO and elevations in glio-vascular GFAP and 
      S100ss were observed after PF (P < 0.05), the latter proportional to the increase 
      in VA flow (r = 0.908, P < 0.05). In contrast, biomarkers of neuronal-axonal 
      damage (neuron-specific enolase, neurofilament light-chain, ubiquitin 
      carboxy-terminal hydrolase L1 and tau) did not change (P > 0.05). Collectively, 
      these findings suggest that the cumulative effects of repeated gravitational 
      transitions may promote minor blood-brain barrier disruption, potentially related 
      to the combined effects of haemodynamic (posterior cerebral hyperperfusion) and 
      molecular (systemic oxidative-nitrosative) stress.
CI  - Copyright (c) 2020 IBRO. Published by Elsevier Ltd. All rights reserved.
FAU - Bailey, Damian M
AU  - Bailey DM
AD  - Neurovascular Research Laboratory, Faculty of Life Sciences and Education, 
      University of South Wales, Glamorgan, UK. Electronic address: 
      damian.bailey@southwales.ac.uk.
FAU - Laneelle, Damien
AU  - Laneelle D
AD  - Service de Medecine Vasculaire, Centre Hospitalo-Universitaire, Caen, France; 
      UNICAEN, INSERM, COMETE, GIP CYCERON, Normandie University, Caen, France.
FAU - Trihan, Jean-Eudes
AU  - Trihan JE
AD  - Service de Medecine Vasculaire, Centre Hospitalier Universitaire de Poitiers, 
      Poitiers, France.
FAU - Marchi, Nicola
AU  - Marchi N
AD  - UMR, Laboratory of Cerebrovascular and Glia Research, Department of Neuroscience, 
      Institute of Functional Genomics (CNRS Unit Mixte de Recherche 5203; INSERM 
      U1191), University of Montpellier, France.
FAU - Stacey, Benjamin S
AU  - Stacey BS
AD  - Neurovascular Research Laboratory, Faculty of Life Sciences and Education, 
      University of South Wales, Glamorgan, UK.
FAU - Tamiya, Kazuki
AU  - Tamiya K
AD  - Department of Biomedical Engineering, Toyo University, Kawagoe-Shi, Saitama, 
      Japan.
FAU - Washio, Takuro
AU  - Washio T
AD  - Department of Biomedical Engineering, Toyo University, Kawagoe-Shi, Saitama, 
      Japan.
FAU - Tuaillon, Edouard
AU  - Tuaillon E
AD  - Unit Mixte de Recherche, INSERM l'Etablissement Francais du Sang, University of 
      Montpellier 1, Montpellier, France.
FAU - Hirtz, Christophe
AU  - Hirtz C
AD  - LBPC-PPC, University of Montpellier, Institute of Regenerative 
      Medicine-Biotherapy IRMB, Centre Hospitalier Universitaire de Montpellier, 
      INSERM, Montpellier, France.
FAU - Lehmann, Sylvain
AU  - Lehmann S
AD  - LBPC-PPC, University of Montpellier, Institute of Regenerative 
      Medicine-Biotherapy IRMB, Centre Hospitalier Universitaire de Montpellier, 
      INSERM, Montpellier, France.
FAU - Ogoh, Shigehiko
AU  - Ogoh S
AD  - Neurovascular Research Laboratory, Faculty of Life Sciences and Education, 
      University of South Wales, Glamorgan, UK; Department of Biomedical Engineering, 
      Toyo University, Kawagoe-Shi, Saitama, Japan.
FAU - Normand, Herve
AU  - Normand H
AD  - UNICAEN, INSERM, COMETE, GIP CYCERON, Normandie University, Caen, France; 
      UNICAEN, COMETE, Caen, France; INSERM, U 1075 COMETE, Caen, France; Department of 
      Clinical Physiology, Centre Hospitalier Universitaire de Caen, Caen, France.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200602
PL  - United States
TA  - Neuroscience
JT  - Neuroscience
JID - 7605074
RN  - 0 (Free Radicals)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - *Cerebrovascular Circulation
MH  - Free Radicals
MH  - Humans
MH  - Middle Cerebral Artery
MH  - *Nitrosative Stress
MH  - Oxidative Stress
MH  - Young Adult
OTO - NOTNLM
OT  - blood-brain barrier
OT  - cerebral blood flow
OT  - free radicals
OT  - gravity
OT  - nitric oxide
EDAT- 2020/06/06 06:00
MHDA- 2021/05/15 06:00
CRDT- 2020/06/06 06:00
PHST- 2020/02/15 00:00 [received]
PHST- 2020/05/22 00:00 [revised]
PHST- 2020/05/26 00:00 [accepted]
PHST- 2020/06/06 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
PHST- 2020/06/06 06:00 [entrez]
AID - S0306-4522(20)30350-X [pii]
AID - 10.1016/j.neuroscience.2020.05.048 [doi]
PST - ppublish
SO  - Neuroscience. 2020 Aug 10;441:142-160. doi: 10.1016/j.neuroscience.2020.05.048. 
      Epub 2020 Jun 2.