PMID- 32502838 OWN - NLM STAT- MEDLINE DCOM- 20210301 LR - 20210326 IS - 1950-6007 (Electronic) IS - 0753-3322 (Linking) VI - 128 DP - 2020 Aug TI - Fufang-Zhenzhu-Tiaozhi capsule ameliorates rabbit's iliac artery restenosis by regulating adiponectin signaling pathway. PG - 110311 LID - S0753-3322(20)30503-5 [pii] LID - 10.1016/j.biopha.2020.110311 [doi] AB - BACKGROUND AND PURPOSE: Fufang-Zhenzhu-Tiaozhi Capsule (FTZ), a traditional Chinese medicine, has been shown obvious effects on the treatment of dyslipidemia and atherosclerosis. The aim of this study was to evaluate whether FTZ can ameliorate rabbit iliac artery restenosis after angioplasty by regulating adiponectin signaling pathway. EXPERIMENTAL APPROACH: The rabbit iliac artery restenosis model was established through percutaneous iliac artery transluminal balloon angioplasty and a high-fat diet. Twenty eight male New Zealand rabbits (8-week-old) were divided into sham operation group (Group Ⅰ), model group (Group Ⅱ), atorvastatin group (Group Ⅲ) and FTZ group (Group Ⅳ), with 7 rabbits in each group. Vascular stenosis was analyzed with Digital Subtraction Angiography. Level of adiponectin (APN), and inflammatory factor including interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha) as well as monocyte chemoattractant protein-1 (MCP-1) was measured by Enzyme Linked Immunosorbent Assay; and injured iliac artery was collected for Hematoxylin-eosin staining and Western Blotting detection of expression of peroxisome proliferator-activated receptor-alpha (PPAR-alpha), adenosine 5'-monophosphate -activated protein kinase (AMPK) and phosphorylated adenosine 5'-monophosphate -activated protein kinase (p-AMPK). Besides, we evaluated FTZ's safety for the first time. KEY RESULTS: Percutaneous iliac artery transluminal balloon angioplasty and high-fat diet result in inflammatory response and restenosis. Compared with Group Ⅱ, iliac artery restenosis was significantly ameliorated in Group Ⅳ (P < 0.05). Treated with FTZ, serum lipids were significantly decreased (P < 0.01), while the level of APN was elevated significantly (P < 0.01). Western blotting detection of the injured iliac artery showed that the expressions of PPAR-alpha, AMPK and p-AMPK were significantly increased in Group Ⅳ (P < 0.01) than that in Group Ⅱ. Besides, before and after taking drugs, liver and kidney function indicators, creatine kinase, as well as measurement of echocardiography were of no statistical difference in four groups(P > 0.05). CONCLUSIONS AND IMPLICATIONS: FTZ could effectively reduce serum lipids and ameliorate rabbit's iliac artery restenosis after angioplasty, and its mechanism may be related to activation of APN signaling pathway. CI - Copyright (c) 2020 The Authors. Published by Elsevier Masson SAS.. All rights reserved. FAU - Li, Tudi AU - Li T AD - Department of Cardiovascular Diseases, the First Affiliated Hospital of Guangdong Pharmaceutical University, China. FAU - Zhang, Rendan AU - Zhang R AD - Department of Cardiovascular Diseases, the First Affiliated Hospital of Guangdong Pharmaceutical University, China. FAU - Liu, Yuhong AU - Liu Y AD - Department of Cardiovascular Diseases, the First Affiliated Hospital of Guangdong Pharmaceutical University, China. FAU - Yao, Yusi AU - Yao Y AD - Department of Cardiovascular Diseases, the First Affiliated Hospital of Guangdong Pharmaceutical University, China. FAU - Guo, Jiao AU - Guo J AD - Guangdong Pharmaceutical University, China. Electronic address: guojiao@gzucm.edu.cn. FAU - Zeng, Zhihuan AU - Zeng Z AD - Department of Cardiovascular Diseases, the First Affiliated Hospital of Guangdong Pharmaceutical University, China. Electronic address: gzzh@163.com. LA - eng PT - Journal Article DEP - 20200602 PL - France TA - Biomed Pharmacother JT - Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie JID - 8213295 RN - 0 (Adiponectin) RN - 0 (Drugs, Chinese Herbal) RN - 0 (Inflammation Mediators) RN - 0 (PPAR alpha) RN - 0 (zhenshu tiaozhi formula) RN - EC 2.7.11.31 (AMP-Activated Protein Kinases) SB - IM MH - AMP-Activated Protein Kinases/metabolism MH - Adiponectin/*blood MH - Angioplasty, Balloon MH - Animals MH - Arterial Occlusive Diseases/blood/*drug therapy/etiology/pathology MH - Diet, High-Fat MH - Disease Models, Animal MH - Drugs, Chinese Herbal/pharmacology/*therapeutic use MH - Iliac Artery/*drug effects/injuries/metabolism/pathology MH - Inflammation Mediators/blood MH - Male MH - PPAR alpha/metabolism MH - Phosphorylation MH - Rabbits MH - Recurrence MH - Signal Transduction MH - Vascular System Injuries/blood/*drug therapy/etiology/pathology OTO - NOTNLM OT - Adiponectin signal pathway OT - FTZ OT - Lipid OT - Restenosis after angioplasty COIS- Declaration of Competing Interest None. EDAT- 2020/06/06 06:00 MHDA- 2021/03/02 06:00 CRDT- 2020/06/06 06:00 PHST- 2019/12/31 00:00 [received] PHST- 2020/05/17 00:00 [revised] PHST- 2020/05/19 00:00 [accepted] PHST- 2020/06/06 06:00 [pubmed] PHST- 2021/03/02 06:00 [medline] PHST- 2020/06/06 06:00 [entrez] AID - S0753-3322(20)30503-5 [pii] AID - 10.1016/j.biopha.2020.110311 [doi] PST - ppublish SO - Biomed Pharmacother. 2020 Aug;128:110311. doi: 10.1016/j.biopha.2020.110311. Epub 2020 Jun 2.