PMID- 32506750
OWN - NLM
STAT- MEDLINE
DCOM- 20210902
LR  - 20210902
IS  - 1600-0897 (Electronic)
IS  - 1046-7408 (Print)
IS  - 1046-7408 (Linking)
VI  - 84
IP  - 3
DP  - 2020 Sep
TI  - Erythropoietin prevents LPS-induced preterm birth and increases offspring 
      survival.
PG  - e13283
LID - 10.1111/aji.13283 [doi]
LID - e13283
AB  - PROBLEM: Preterm delivery is the leading cause of neonatal mortality and 
      contributes to delayed physical and cognitive development in children. At 
      present, there is no efficient therapy to prevent preterm labor. A large body of 
      evidence suggests that infections might play a significant and potentially 
      preventable cause of premature birth. This work assessed the effects of 
      erythropoietin (EPO) in a murine model of inflammation-associated preterm 
      delivery, which mimics central features of preterm infections in humans. METHOD 
      OF STUDY: BALB/c mice were injected i.p. with 20 000 IU/kg EPO or normal saline 
      twice on gestational day (GD) 15, with a 3 hours time interval between 
      injections. An hour after the first EPO or normal saline injection, all mice 
      received two injections of 50 mug/kg LPS, also given 3 hours apart. RESULTS: EPO 
      significantly prevented preterm labor and increased offspring survival in an LPS 
      induced preterm delivery model. EPO prevented LPS-induced leukocyte infiltration 
      into the placenta. Moreover, EPO inhibited the expression of pro-inflammatory 
      cytokines, interleukin-1beta (IL-1beta), interleukin-6 (IL-6), and tumour necrosis 
      factor-alpha (TNF-alpha) in maternal serum and amniotic fluid. EPO also prevented 
      LPS-induced increase in placental prostaglandin (PG)E2 and uterine inducible 
      nitric oxide synthase (iNOS) production, while decreasing nuclear factor kappa-B 
      (NF-kappabeta) activity in the myometrium. EPO also increased the gene expression of 
      placental programmed cell death ligand 1 (PD-L1) in LPS-treated mice. 
      CONCLUSIONS: Our results suggest that EPO could be a potential novel therapeutic 
      strategy to tackle infection-related preterm labor.
CI  - (c) 2020 The Authors. American Journal of Reproductive Immunology published by John 
      Wiley & Sons Ltd.
FAU - Zhang, Jie
AU  - Zhang J
AUID- ORCID: 0000-0002-3935-5883
AD  - Department of Rehabilitation, Guangdong Women and Children Hospital, Guangzhou, 
      China.
FAU - Luo, Xianqiong
AU  - Luo X
AD  - Department of Pediatrics, Guangdong Women and Children Hospital, Guangzhou, 
      China.
FAU - Huang, Caicai
AU  - Huang C
AD  - Department of Obstetrics, Guangdong Women and Children Hospital, Guangzhou, 
      China.
FAU - Pei, Zheng
AU  - Pei Z
AD  - Department of Rehabilitation, Guangdong Women and Children Hospital, Guangzhou, 
      China.
FAU - Xiao, Huimei
AU  - Xiao H
AD  - Department of Rehabilitation, Guangdong Women and Children Hospital, Guangzhou, 
      China.
FAU - Luo, Xingang
AU  - Luo X
AD  - Department of Rehabilitation, Guangdong Women and Children Hospital, Guangzhou, 
      China.
FAU - Huang, Shuangmiao
AU  - Huang S
AD  - Department of Rehabilitation, Guangdong Women and Children Hospital, Guangzhou, 
      China.
FAU - Chang, Yanqun
AU  - Chang Y
AD  - Department of Rehabilitation, Guangdong Women and Children Hospital, Guangzhou, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20200711
PL  - Denmark
TA  - Am J Reprod Immunol
JT  - American journal of reproductive immunology (New York, N.Y. : 1989)
JID - 8912860
RN  - 0 (B7-H1 Antigen)
RN  - 0 (Cd274 protein, mouse)
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 11096-26-7 (Erythropoietin)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.13.39 (Nos2 protein, mouse)
RN  - K7Q1JQR04M (Dinoprostone)
SB  - IM
MH  - Amniotic Fluid/immunology
MH  - Animals
MH  - B7-H1 Antigen/immunology
MH  - Cytokines/blood
MH  - Dinoprostone/immunology
MH  - Erythropoietin/*therapeutic use
MH  - Female
MH  - Leukocytes/drug effects/immunology
MH  - Lipopolysaccharides
MH  - Mice, Inbred BALB C
MH  - NF-kappa B/immunology
MH  - Nitric Oxide Synthase Type II/immunology
MH  - Placenta/drug effects/immunology
MH  - Pregnancy
MH  - Pregnancy Complications, Infectious/immunology/*prevention & control
MH  - Premature Birth/chemically induced/immunology/*prevention & control
PMC - PMC7507205
OTO - NOTNLM
OT  - PDL1
OT  - erythropoietin
OT  - inflammation
OT  - offspring survival
OT  - preterm birth
OT  - prostaglandins
COIS- The authors declare that they have no conflict of interest.
EDAT- 2020/06/09 06:00
MHDA- 2021/09/03 06:00
PMCR- 2020/09/22
CRDT- 2020/06/08 06:00
PHST- 2019/11/27 00:00 [received]
PHST- 2020/05/13 00:00 [revised]
PHST- 2020/06/01 00:00 [accepted]
PHST- 2020/06/09 06:00 [pubmed]
PHST- 2021/09/03 06:00 [medline]
PHST- 2020/06/08 06:00 [entrez]
PHST- 2020/09/22 00:00 [pmc-release]
AID - AJI13283 [pii]
AID - 10.1111/aji.13283 [doi]
PST - ppublish
SO  - Am J Reprod Immunol. 2020 Sep;84(3):e13283. doi: 10.1111/aji.13283. Epub 2020 Jul 
      11.