PMID- 32509076
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200611
IS  - 1936-2625 (Electronic)
IS  - 1936-2625 (Linking)
VI  - 13
IP  - 5
DP  - 2020
TI  - Semi-comprehensive analysis of gene amplification in thymic malignant tumors 
      using multiplex ligation-dependent probe amplification and fluorescence in situ 
      hybridization.
PG  - 1035-1044
AB  - Research on the amplification of oncogenes in thymic malignant tumor is limited. 
      In this study, we aimed to determine the gene amplification status of receptor 
      tyrosine kinases and other cell regulator genes in thymic malignant tumors, with 
      a view toward the future introduction of molecular targeted therapy. In addition, 
      we examined the usefulness of multiplex, ligation-dependent probe amplification 
      (MLPA) in the semi-comprehensive detection of these gene amplifications. The 
      participants of this study were nine patients with thymic carcinoma and one 
      patient with atypical carcinoid who underwent resection at our department from 
      1999 to 2016. Twenty-four oncogenes (MDM4, MYCN, ALK, PDGFRA, KIT, KDR, DHFR, 
      EGFR, MET, SMO, BRAF, FGFR1, MYC, ABL1, RET, CCND1, CCND2, CDK4, MDM2, AURKB, 
      ERBB2, TOP2A, AURKA, AR) were analyzed for amplification by MLPA. In cases where 
      amplification by MLPA was suspected, confirmation was performed by fluorescence 
      in situ hybridization (FISH). Immunostaining for detected oncoproteins and p53 
      were performed in cases with confirmed oncogene amplification. MYC (2/10, 20%) 
      and MDM2 (1/10, 10%) amplifications were detected using MLPA and FISH. 
      Immunostaining in both cases was positive. The MDM2-amplified tumor relapsed and 
      spread rapidly after operation despite the use of post-operative 
      chemo-radiotherapy. MYC amplification may be involved in the carcinogenesis of 
      thymic malignant tumors. In addition, MDM2 amplification may be a concern in the 
      increased malignancy.
CI  - IJCEP Copyright (c) 2020.
FAU - Kakegawa, Seiichi
AU  - Kakegawa S
AD  - Department of Thoracic, Cardiovascular and General Surgery, Graduate School of 
      Medical Science, Kanazawa University Ishikawa, Japan.
FAU - Matsumoto, Isao
AU  - Matsumoto I
AD  - Department of Thoracic, Cardiovascular and General Surgery, Graduate School of 
      Medical Science, Kanazawa University Ishikawa, Japan.
FAU - Tamura, Masaya
AU  - Tamura M
AD  - Department of Thoracic, Cardiovascular and General Surgery, Graduate School of 
      Medical Science, Kanazawa University Ishikawa, Japan.
FAU - Takata, Munehisa
AU  - Takata M
AD  - Department of Thoracic, Cardiovascular and General Surgery, Graduate School of 
      Medical Science, Kanazawa University Ishikawa, Japan.
FAU - Yoshida, Shuhei
AU  - Yoshida S
AD  - Department of Thoracic, Cardiovascular and General Surgery, Graduate School of 
      Medical Science, Kanazawa University Ishikawa, Japan.
FAU - Saito, Daisuke
AU  - Saito D
AD  - Department of Thoracic, Cardiovascular and General Surgery, Graduate School of 
      Medical Science, Kanazawa University Ishikawa, Japan.
FAU - Tanaka, Yusuke
AU  - Tanaka Y
AD  - Department of Thoracic, Cardiovascular and General Surgery, Graduate School of 
      Medical Science, Kanazawa University Ishikawa, Japan.
FAU - Takemura, Hirofumi
AU  - Takemura H
AD  - Department of Thoracic, Cardiovascular and General Surgery, Graduate School of 
      Medical Science, Kanazawa University Ishikawa, Japan.
FAU - Ooi, Akishi
AU  - Ooi A
AD  - Department of Molecular and Cellular Pathology, Graduate School of Medical 
      Science, Kanazawa University Ishikawa, Japan.
LA  - eng
PT  - Journal Article
DEP - 20200501
PL  - United States
TA  - Int J Clin Exp Pathol
JT  - International journal of clinical and experimental pathology
JID - 101480565
PMC - PMC7270679
OTO - NOTNLM
OT  - MDM2
OT  - MYC
OT  - Thymic carcinoma
OT  - fluorescence in situ hybridization
OT  - gene amplification
OT  - multiplex ligation-dependent probe amplification
COIS- None.
EDAT- 2020/06/09 06:00
MHDA- 2020/06/09 06:01
PMCR- 2020/05/01
CRDT- 2020/06/09 06:00
PHST- 2020/01/31 00:00 [received]
PHST- 2020/03/27 00:00 [accepted]
PHST- 2020/06/09 06:00 [entrez]
PHST- 2020/06/09 06:00 [pubmed]
PHST- 2020/06/09 06:01 [medline]
PHST- 2020/05/01 00:00 [pmc-release]
PST - epublish
SO  - Int J Clin Exp Pathol. 2020 May 1;13(5):1035-1044. eCollection 2020.