PMID- 32509200
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1943-8141 (Print)
IS  - 1943-8141 (Electronic)
IS  - 1943-8141 (Linking)
VI  - 12
IP  - 5
DP  - 2020
TI  - Quercetin stimulates osteogenic differentiation of bone marrow stromal cells 
      through miRNA-206/connexin 43 pathway.
PG  - 2062-2070
AB  - The quantity and function of osteoblasts require continuous osteogenic 
      differentiation of bone marrow mesenchymal stem cells. Recent evidence suggests 
      that microRNAs (miRNAs) act as important post-transcriptional regulators in a 
      wide range of biological processes, including osteoblastic differentiation. 
      Quercetin has also been found to prevent bone loss. In this study, we 
      investigated the osteogenesis of quercetin and miR-206 on bone marrow mesenchymal 
      stem cells (BMSCs) and their relationship. We observed quercetin enhanced BMSCs 
      proliferation with a dose-dependent manner in Cell Counting Kit-8 (CCK-8). 
      Alizarin red S staining, alkaline phosphatase (ALP) quantification assay, miR-206 
      and mRNA levels of osteogenesis marker genes by quantitative real-time PCR (qPCR) 
      were used to analyze osteogenic potential. We observed quercetin significantly 
      elevated bone mineralization and the mRNA expression levels of 
      osteoblast-specific genes including Runt-related transcription factor 2 (Runx2), 
      Osterix (OSX), osteocalcin (OCN), and osteopontin (OPN). Correspondly, Cx43 
      expression were increased, while miR-206 expression were decreased. In the 
      presence of agomir of miR-206, effects of quercetin on mineralization, alkaline 
      phosphatase activity and osteoblast-specific genes expression were suppressed. 
      Most of all, Cx43 protein level was also blocked while overexpression of miR-206 
      against quercetin effects. Taken together, these data indicated that quercetin 
      promotes BMSCs proliferation and osteogenic differentiation. The osteogenic 
      effect of quercetin is partly modulated through miR-206/Cx43 pathway.
CI  - AJTR Copyright (c) 2020.
FAU - Zhang, Qihao
AU  - Zhang Q
AD  - Department of Orthopedics, The Third Affiliated Hospital (The Affiliated Luohu 
      Hospital) of Shenzhen University Shenzhen 518000, Guangdong, P. R. China.
FAU - Chang, Bo
AU  - Chang B
AD  - Department of Orthopedics, The Third Affiliated Hospital (The Affiliated Luohu 
      Hospital) of Shenzhen University Shenzhen 518000, Guangdong, P. R. China.
FAU - Zheng, Guizhou
AU  - Zheng G
AD  - Department of Orthopedics, The Third Affiliated Hospital (The Affiliated Luohu 
      Hospital) of Shenzhen University Shenzhen 518000, Guangdong, P. R. China.
FAU - Du, Shixin
AU  - Du S
AD  - Department of Orthopedics, The Third Affiliated Hospital (The Affiliated Luohu 
      Hospital) of Shenzhen University Shenzhen 518000, Guangdong, P. R. China.
FAU - Li, Xuedong
AU  - Li X
AD  - Department of Orthopedics, The Third Affiliated Hospital (The Affiliated Luohu 
      Hospital) of Shenzhen University Shenzhen 518000, Guangdong, P. R. China.
LA  - eng
PT  - Journal Article
DEP - 20200515
PL  - United States
TA  - Am J Transl Res
JT  - American journal of translational research
JID - 101493030
PMC - PMC7270039
OTO - NOTNLM
OT  - Quercetin
OT  - bone marrow stromal cells
OT  - connexin 43
OT  - miR-206
OT  - osteogenic differentiation
COIS- None.
EDAT- 2020/06/09 06:00
MHDA- 2020/06/09 06:01
PMCR- 2020/05/15
CRDT- 2020/06/09 06:00
PHST- 2019/05/15 00:00 [received]
PHST- 2020/04/16 00:00 [accepted]
PHST- 2020/06/09 06:00 [entrez]
PHST- 2020/06/09 06:00 [pubmed]
PHST- 2020/06/09 06:01 [medline]
PHST- 2020/05/15 00:00 [pmc-release]
PST - epublish
SO  - Am J Transl Res. 2020 May 15;12(5):2062-2070. eCollection 2020.