PMID- 32510153
OWN - NLM
STAT- MEDLINE
DCOM- 20210106
LR  - 20210106
IS  - 1745-7270 (Electronic)
IS  - 1672-9145 (Linking)
VI  - 52
IP  - 9
DP  - 2020 Sep 8
TI  - miR-20b-5p attenuates hypoxia-induced apoptosis in cardiomyocytes via the 
      HIF-1alpha/NF-kappaB pathway.
PG  - 927-934
LID - 10.1093/abbs/gmaa056 [doi]
AB  - Chronic hypoxia is a common inducer of end-stage cardiovascular disease. In cells 
      under hypoxia, the hypoxia-inducible factor-1 (HIF-1) plays a vital role in 
      regulating downstream target genes. However, the mechanism of hypoxia in 
      cardiomyocytes is still unclear. In this study, we aimed to identify novel 
      downstream epigenetic targets of HIF-1alpha in cardiomyocytes under hypoxia. H9c2 
      cells were exposed to hypoxia condition, and quantitative real-time PCR analysis 
      was performed to evaluate the expression of miR-20b-5p. The results indicated 
      that the expression of miR-20b-5p was down-regulated in H9c2 cells under low 
      oxygen condition. Meanwhile, HIF-1alpha overexpression further down-regulated the 
      miR-20b-5p expression in H9c2 cells transfected with HIF-1alpha plasmids. In 
      addition, Annexin-V-FITC/PI flow cytometry analysis suggested that overexpression 
      of miR-20b-5p attenuated cell apoptosis under hypoxia condition in H9c2 cells. 
      Western blot analysis showed that the hypoxia apparently increased Bax and 
      cleaved-caspase-3, but decreased Bcl-2 expression in H9c2 cells, indicating that 
      hypoxia-induced NF-kappaB signaling pathway activation is mediated by miR-20b-5p. 
      Hypoxia-induced H9c2 cell apoptosis was reduced after HIF-1alpha knockdown as shown 
      by the flow cytometry analysis. In conclusion, we identified that miR-20b-5p 
      plays an important role in mediating cardiomyocytes apoptosis under hypoxia, 
      which is mediated by the HIF-1/NF-kappaB signaling pathway.
CI  - (c) The Author(s) 2020. Published by Oxford University Press on behalf of the 
      Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological 
      Sciences, Chinese Academy of Sciences. All rights reserved. For permissions, 
      please e-mail: journals.permissions@oup.com.
FAU - Zhou, Zhongquan
AU  - Zhou Z
AD  - Department of Cardiology, The First Hospital of Jingzhou City (the First 
      Affiliated Hospital of Yangtze University), Jingzhou 434000, China.
FAU - Chen, Songwen
AU  - Chen S
AD  - Department of Cardiology, Shanghai General Hospital, Shanghai 200000, China.
FAU - Tian, Zhiming
AU  - Tian Z
AD  - Department of Cardiology, First School of Clinical Medicine College, Yangtze 
      University, Jingzhou 434000, China.
FAU - Deng, Shibing
AU  - Deng S
AD  - Department of Cardiology, The First Hospital of Jingzhou City (the First 
      Affiliated Hospital of Yangtze University), Jingzhou 434000, China.
FAU - Yi, Xuying
AU  - Yi X
AD  - Department of Cardiology, The First Hospital of Jingzhou City (the First 
      Affiliated Hospital of Yangtze University), Jingzhou 434000, China.
FAU - Yang, Shaning
AU  - Yang S
AD  - Department of Cardiology, The First Hospital of Jingzhou City (the First 
      Affiliated Hospital of Yangtze University), Jingzhou 434000, China.
FAU - Yang, Xuexin
AU  - Yang X
AD  - Department of Cardiology, The First Hospital of Jingzhou City (the First 
      Affiliated Hospital of Yangtze University), Jingzhou 434000, China.
FAU - Jin, Lijun
AU  - Jin L
AD  - Department of Cardiology, The First Hospital of Jingzhou City (the First 
      Affiliated Hospital of Yangtze University), Jingzhou 434000, China.
FAU - Shi, Wanqing
AU  - Shi W
AD  - The First Hospital of Jingzhou City, The Interventional Center, Jingzhou 434000, 
      China.
LA  - eng
PT  - Journal Article
PL  - China
TA  - Acta Biochim Biophys Sin (Shanghai)
JT  - Acta biochimica et biophysica Sinica
JID - 101206716
RN  - 0 (Hif1a protein, rat)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (NF-kappa B)
SB  - IM
MH  - Aged
MH  - Animals
MH  - *Apoptosis
MH  - Cell Hypoxia
MH  - Cell Line
MH  - Female
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/*biosynthesis/genetics
MH  - Male
MH  - Middle Aged
MH  - Myocytes, Cardiac/*metabolism/pathology
MH  - NF-kappa B/genetics/*metabolism
MH  - Rats
MH  - *Signal Transduction
OTO - NOTNLM
OT  - NF-kappaB
OT  - cardiomyocytes
OT  - hypoxia
OT  - hypoxia-inducible factor-1
OT  - miR-20b-5p
EDAT- 2020/06/09 06:00
MHDA- 2021/01/07 06:00
CRDT- 2020/06/09 06:00
PHST- 2019/10/29 00:00 [received]
PHST- 2019/10/17 00:00 [revised]
PHST- 2019/12/26 00:00 [accepted]
PHST- 2020/06/09 06:00 [pubmed]
PHST- 2021/01/07 06:00 [medline]
PHST- 2020/06/09 06:00 [entrez]
AID - 5854389 [pii]
AID - 10.1093/abbs/gmaa056 [doi]
PST - ppublish
SO  - Acta Biochim Biophys Sin (Shanghai). 2020 Sep 8;52(9):927-934. doi: 
      10.1093/abbs/gmaa056.