PMID- 32510484 OWN - NLM STAT- MEDLINE DCOM- 20201007 LR - 20201007 IS - 1940-087X (Electronic) IS - 1940-087X (Linking) IP - 159 DP - 2020 May 23 TI - Preference Assessment of White-backed Planthopper Feeding on Rice. LID - 10.3791/59323 [doi] AB - Exploiting insect-resistant rice germplasm resources and related genes is the primary need for breeding insect-resistant varieties, but the accuracy of the identification of insect-resistant phenotypes of rice is a major difficulty. It is urgent to develop a new method or improve existing methods to screen rice for insect resistance. This article describes a simple and feasible method to assess nonpreference-type resistance of rice to the white-backed planthopper (WBPH), Sogatella furcifera, in the laboratory. The preference of adult WBPHs feeding or inhabiting on maturing rice plants is continuously analyzed by pairwise comparison. The dynamic changes of WBPHs on rice plants are recorded and compared as an index of resistance identification. The current method is simply operable and easily observable and has a short cycle. The use of this method could be extended to investigate the feeding and oviposition preference of similar hemipterans, such as the brown planthopper (BPH), Nilaparvata lugens(Stal). FAU - Zhang, Lei AU - Zhang L AD - Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences. FAU - Wu, Chao AU - Wu C AD - Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences. FAU - Xiao, Yutao AU - Xiao Y AD - Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences; xiao20020757@163.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Video-Audio Media DEP - 20200523 PL - United States TA - J Vis Exp JT - Journal of visualized experiments : JoVE JID - 101313252 SB - IM MH - Animals MH - *Feeding Behavior MH - Female MH - Hemiptera/*physiology MH - Laboratories MH - *Oryza MH - Oviposition MH - Phenotype EDAT- 2020/06/09 06:00 MHDA- 2020/10/08 06:00 CRDT- 2020/06/09 06:00 PHST- 2020/06/09 06:00 [entrez] PHST- 2020/06/09 06:00 [pubmed] PHST- 2020/10/08 06:00 [medline] AID - 10.3791/59323 [doi] PST - epublish SO - J Vis Exp. 2020 May 23;(159). doi: 10.3791/59323.