PMID- 32514247
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20230717
IS  - 1475-2867 (Print)
IS  - 1475-2867 (Electronic)
IS  - 1475-2867 (Linking)
VI  - 20
DP  - 2020
TI  - Circular RNA circ-CSPP1 regulates CCNE2 to facilitate hepatocellular carcinoma 
      cell growth via sponging miR-577.
PG  - 202
LID - 10.1186/s12935-020-01287-8 [doi]
LID - 202
AB  - OBJECTIVE: Circ-centro-some/spindle pole-associated protein (CSPP1) has been 
      confirmed to be characterized in diverse human malignancies and its ectopic 
      expression may regulate tumor progression and development. However, in 
      hepatocellular carcinoma (HCC), its biological role, clinical significance and 
      molecular mechanism are still unclear. METHODS: Circ-CSPP1 expression and its 
      prognostic values in HCC tissues were detected by qRT-PCR or in situ 
      hybridization (ISH), and enriched by using Rnase R. The functional experiments 
      (Circ-CSPP1 was overexpressed or knocked down) were performed in HCC cells. The 
      HCC cell growth was analyzed by CCK-8 assay, transwell, wound healing and colony 
      formation assays. The interation between circ-CSPP1 and miR-577/miR-577 and 
      cyclin E2 (CCNE2) were determined by dual luciferase assay or RNA binding protein 
      immunoprecipitation (RIP) assay. The RNA fluorescence in situ hybridization 
      (FISH) assay was used to detect the subcellular distribution. Finally, an in vivo 
      nude mouse tumor model was constructed. RESULTS: In HCC patients and cells, 
      circ-CSPP1 was aberrantly expressed, and its upregulation predicted poor 
      prognosis, and closely correlated with tumor size and TNM stage. Circ-CSPP1 
      resisted RnaseR digestion, indicating it is a circular RNA structure. Moreover, 
      overexpression of circ-CSPP1 promoted HCC cell viability, colony formation, 
      migration, and invasion in vitro. Knockdown of circ-CSPP1 showed contrary 
      results. Circ-CSPP1 acts as a miR-577 sponge and positively regulated the target 
      of miR-577, CCNE2. Besides, miR-577 inhibitor rescued the suppressive effects of 
      circ-CSPP1 knockdown on HCC cell growth, whereas was completely reversed by 
      silencing of CCNE2. Finally, the in vivo experiments confirmed that circ-CSPP1 
      knockdown regulated xenograft tumor volume and downregulated CCNE2, p-Rb, E2F1 
      and c-myc expression. CONCLUSION: These findings revealed that circ-CSPP1 
      contributed to HCC progression by positively regulating CCNE2 via miR-577, thus 
      established its potential as new a prognostic and therapeutic marker for HCC 
      patients.
CI  - (c) The Author(s) 2020.
FAU - Sun, Qian
AU  - Sun Q
AUID- ORCID: 0000-0001-9786-374X
AD  - Department of Gastroenterology and Hepatology, The First Affiliated Hospital of 
      Zhengzhou University, 1 Jianshe Dong Lu, Erqi District, Zhengzhou, Henan 450052 
      China. GRID: grid.412633.1
FAU - Yu, Rui
AU  - Yu R
AD  - Department of Gastroenterology and Hepatology, The First Affiliated Hospital of 
      Zhengzhou University, 1 Jianshe Dong Lu, Erqi District, Zhengzhou, Henan 450052 
      China. GRID: grid.412633.1
FAU - Wang, Chunfeng
AU  - Wang C
AD  - Department of Gastroenterology and Hepatology, The First Affiliated Hospital of 
      Zhengzhou University, 1 Jianshe Dong Lu, Erqi District, Zhengzhou, Henan 450052 
      China. GRID: grid.412633.1
FAU - Yao, Jianning
AU  - Yao J
AD  - Department of Gastroenterology and Hepatology, The First Affiliated Hospital of 
      Zhengzhou University, 1 Jianshe Dong Lu, Erqi District, Zhengzhou, Henan 450052 
      China. GRID: grid.412633.1
FAU - Zhang, Lianfeng
AU  - Zhang L
AD  - Department of Gastroenterology and Hepatology, The First Affiliated Hospital of 
      Zhengzhou University, 1 Jianshe Dong Lu, Erqi District, Zhengzhou, Henan 450052 
      China. GRID: grid.412633.1
LA  - eng
PT  - Journal Article
PT  - Retracted Publication
DEP - 20200529
PL  - England
TA  - Cancer Cell Int
JT  - Cancer cell international
JID - 101139795
EIN - Cancer Cell Int. 2020 Jul 8;20:293. PMID: 32669969
RIN - Cancer Cell Int. 2023 Jul 17;23(1):140. PMID: 37460935
PMC - PMC7260814
OTO - NOTNLM
OT  - CCNE2
OT  - Circular RNA circ-CSPP1
OT  - Hepatocellular carcinoma
OT  - MiR-577
COIS- Competing interestsThe authors declare that they have no competing interests, and 
      all authors should confirm its accuracy.
EDAT- 2020/06/10 06:00
MHDA- 2020/06/10 06:01
PMCR- 2020/05/29
CRDT- 2020/06/10 06:00
PHST- 2020/04/08 00:00 [received]
PHST- 2020/05/22 00:00 [accepted]
PHST- 2020/06/10 06:00 [entrez]
PHST- 2020/06/10 06:00 [pubmed]
PHST- 2020/06/10 06:01 [medline]
PHST- 2020/05/29 00:00 [pmc-release]
AID - 1287 [pii]
AID - 10.1186/s12935-020-01287-8 [doi]
PST - epublish
SO  - Cancer Cell Int. 2020 May 29;20:202. doi: 10.1186/s12935-020-01287-8. eCollection 
      2020.