PMID- 32515250
OWN - NLM
STAT- MEDLINE
DCOM- 20210713
LR  - 20210713
IS  - 1532-4281 (Electronic)
IS  - 1079-9893 (Linking)
VI  - 40
IP  - 6
DP  - 2020 Dec
TI  - Curcumin potentiates laryngeal squamous carcinoma radiosensitivity via NF-KappaB 
      inhibition by suppressing IKKgamma expression.
PG  - 541-549
LID - 10.1080/10799893.2020.1767649 [doi]
AB  - Context: Curcumin has shown efficacy in promoting radiosensitivity combined with 
      radiotherapy. However, the role and mechanism of curcumin on radiosensitivity in 
      laryngeal squamous cell cancer (LSCC) is largely unknown.Objective: The aim of 
      our study is to explore the role of IKKgamma-NF-kappaB signaling in curcumin enhancing 
      LSCC cell radiosensitivity in vitro.Materials and methods: Curcumin and X-ray 
      were used to induce cell DNA damage and apoptosis, or inhibit cell clone 
      formation. IKKgamma siRNA and plasmid were used to change IKKgamma expression. The CCK8 
      assay was used to detect cell viability. Clone formation ability was analyzed 
      using a clonogenic assay, cell apoptosis was examined using flow cytometry, an 
      immunofluorescence assay was used to detect DNA damage, while mRNA and protein 
      levels were assayed using real time PCR and western blotting, 
      respectively.Results: Curcumin significantly enhanced irradiation-induced DNA 
      damage and apoptosis, while weakening clone-forming abilities of LSCC cell line 
      Hep2 and Hep2-max. Compared to Hep2 cells, Hep2-max cells are more sensitive to 
      curcumin post-irradiation. Curcumin suppressed irradiation-induced NF-kappaB 
      activation by suppressing IKKgamma expression, but not IKKalpha and IKKbeta. Overexpression 
      of IKKgamma decreased irradiation-induced DNA damage and apoptosis, while promoting 
      clone-forming abilities of Hep2 and Hep2-max cells. IKKgamma overexpression further 
      increased expression of NF-kappaB downstream genes, Bcl-XL, Bcl-2, and cyclin D1. 
      Conversely, IKKgamma silencing enhanced irradiation-induced DNA damage and apoptosis, 
      but promoted clone formation in Hep2 and Hep2-max cells. Additionally, IKKgamma 
      silencing inhibited expression of Bcl-XL, Bcl-2, and cyclin D1.Conclusions: 
      Curcumin enhances LSCC radiosensitivity via NF-KappaB inhibition by suppressing IKKgamma 
      expression.
FAU - Deng, Xin-Zhou
AU  - Deng XZ
AD  - Department of Clinical Oncology, Taihe Hospital, Hubei University of Medicine, 
      Shiyan, Hubei, PR China.
AD  - HubeiKey Laboratory of Embryonic Stem Cell Research, Taihe Hospital, Hubei 
      University of Medicine, Shiyan, Hubei, PR China.
FAU - Geng, Shan-Shan
AU  - Geng SS
AD  - Department of Clinical Oncology, Taihe Hospital, Hubei University of Medicine, 
      Shiyan, Hubei, PR China.
AD  - Postgraduate Training Base, Shiyan Taihe Hospital, Jinzhou Medical University, 
      Shiyan, Hubei, PR China.
FAU - Luo, Ming
AU  - Luo M
AD  - Department of Clinical Oncology, Taihe Hospital, Hubei University of Medicine, 
      Shiyan, Hubei, PR China.
FAU - Chai, Jing-Jing
AU  - Chai JJ
AD  - Department of Clinical Oncology, Taihe Hospital, Hubei University of Medicine, 
      Shiyan, Hubei, PR China.
FAU - Xu, Ying
AU  - Xu Y
AD  - Department of Clinical Oncology, Taihe Hospital, Hubei University of Medicine, 
      Shiyan, Hubei, PR China.
FAU - Chen, Chun-Li
AU  - Chen CL
AD  - Department of Clinical Oncology, Taihe Hospital, Hubei University of Medicine, 
      Shiyan, Hubei, PR China.
FAU - Qiu, Li
AU  - Qiu L
AD  - Department of Clinical Oncology, Taihe Hospital, Hubei University of Medicine, 
      Shiyan, Hubei, PR China.
FAU - Ke, Qing
AU  - Ke Q
AD  - Department of Clinical Oncology, Taihe Hospital, Hubei University of Medicine, 
      Shiyan, Hubei, PR China.
FAU - Duan, Qi-Wen
AU  - Duan QW
AD  - Department of Clinical Oncology, Taihe Hospital, Hubei University of Medicine, 
      Shiyan, Hubei, PR China.
FAU - Song, Shi-Mao
AU  - Song SM
AD  - Department of Clinical Oncology, Taihe Hospital, Hubei University of Medicine, 
      Shiyan, Hubei, PR China.
FAU - Shen, Li
AU  - Shen L
AD  - Department of Biochemistry, School of Basic Medical Sciences, Hubei University of 
      Medicine, Shiyan, Hubei, PR China.
FAU - Luo, Zhi-Guo
AU  - Luo ZG
AD  - Department of Clinical Oncology, Taihe Hospital, Hubei University of Medicine, 
      Shiyan, Hubei, PR China.
LA  - eng
PT  - Journal Article
DEP - 20200609
PL  - England
TA  - J Recept Signal Transduct Res
JT  - Journal of receptor and signal transduction research
JID - 9509432
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (IKBKG protein, human)
RN  - 0 (NF-kappa B)
RN  - 0 (Radiation-Sensitizing Agents)
RN  - EC 2.7.11.10 (I-kappa B Kinase)
RN  - IT942ZTH98 (Curcumin)
SB  - IM
MH  - Antineoplastic Agents/pharmacology
MH  - Apoptosis
MH  - Biomarkers, Tumor/genetics/metabolism
MH  - Carcinoma, Squamous Cell/drug therapy/metabolism/pathology/*radiotherapy
MH  - Cell Proliferation
MH  - Curcumin/*pharmacology
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - I-kappa B Kinase/*antagonists & inhibitors
MH  - Laryngeal Neoplasms/drug therapy/metabolism/pathology/*radiotherapy
MH  - NF-kappa B/*antagonists & inhibitors
MH  - Phosphorylation
MH  - Radiation Tolerance/*drug effects
MH  - Radiation-Sensitizing Agents/*pharmacology
MH  - Signal Transduction
MH  - Tumor Cells, Cultured
OTO - NOTNLM
OT  - Curcumin
OT  - IKKgamma
OT  - NF-kappaB
OT  - laryngeal squamous carcinoma
OT  - radiosensitivity
EDAT- 2020/06/10 06:00
MHDA- 2021/07/14 06:00
CRDT- 2020/06/10 06:00
PHST- 2020/06/10 06:00 [pubmed]
PHST- 2021/07/14 06:00 [medline]
PHST- 2020/06/10 06:00 [entrez]
AID - 10.1080/10799893.2020.1767649 [doi]
PST - ppublish
SO  - J Recept Signal Transduct Res. 2020 Dec;40(6):541-549. doi: 
      10.1080/10799893.2020.1767649. Epub 2020 Jun 9.