PMID- 32515416
OWN - NLM
STAT- MEDLINE
DCOM- 20200610
LR  - 20240425
IS  - 1757-9996 (Electronic)
IS  - 1602-1622 (Linking)
VI  - 18
DP  - 2020 Jul 4
TI  - Genetic Implications of HLA-DR and HLA-DQ Genotype on Tobacco Smoking and Oral 
      Submucous Fibrosis.
PG  - 455-460
LID - 10.3290/j.ohpd.a44683 [doi]
AB  - PURPOSE: Integration of genetic information into our understanding of oral 
      diseases has fostered the hope to intervene the disease process among genetically 
      susceptible individuals. Oral submucous fibrosis (OSF) (mainly in the Southeast 
      Asia region) and tobacco smoking are two of the major public health problems the 
      world is facing today. With more and more diseases being associated with alleles 
      of the human leukocyte antigen (HLA), the objective of the study was to explore 
      any genetic association of OSF and smoking behaviour with specific HLA Class II 
      DQB1*0503 and HLA DRB1*0301 alleles. MATERIALS AND METHODS: Genomic DNA was 
      extracted from saliva of 64 patients divided into an OSF group, a tobacco smokers 
      group and a control group. This was followed by polymerase chain reaction (PCR) 
      with sequence-specific primer of HLA-DQB1*0503 and HLA DRB1*0301 allele, 
      visualised under 2% agarose gel. RESULTS: A statistically significant difference 
      was observed between the OSF group and controls in presence of HLA-DQB1*0503 
      allele, with 84% of the patients showing the presence. Frequency of HLA DRB1*0301 
      allele was also found to be significantly higher (72%) among OSF patients (p 
      <0.001). Similar results were shown in tobacco smokers with 28% cases showing 
      presence of HLA DRB1*0301 allele and 13 (52%) of them having DQB1*0503 allele (p 
      <0.001). CONCLUSION: HLA-DRB1*0301 and HLA-DQB1*0503 are statistically 
      significantly associated with susceptibility to OSF and smoking behaviour.
FAU - Purohit, Abhishek
AU  - Purohit A
FAU - Purohit, Bharathi M
AU  - Purohit BM
FAU - Mani, Abin
AU  - Mani A
FAU - Bhambal, Ajay
AU  - Bhambal A
LA  - eng
PT  - Journal Article
DEP - 20200704
PL  - Germany
TA  - Oral Health Prev Dent
JT  - Oral health & preventive dentistry
JID - 101167768
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (HLA-DRB1 Chains)
SB  - IM
MH  - Gene Frequency
MH  - Genotype
MH  - *HLA-DQ Antigens
MH  - HLA-DRB1 Chains
MH  - Haplotypes
MH  - Humans
MH  - *Oral Submucous Fibrosis
MH  - Tobacco Smoking
OTO - NOTNLM
OT  - HLA
OT  - OSF
OT  - genetics
OT  - tobacco
EDAT- 2020/06/10 06:00
MHDA- 2020/06/11 06:00
CRDT- 2020/06/10 06:00
PHST- 2020/06/10 06:00 [entrez]
PHST- 2020/06/10 06:00 [pubmed]
PHST- 2020/06/11 06:00 [medline]
AID - 842318 [pii]
AID - 10.3290/j.ohpd.a44683 [doi]
PST - epublish
SO  - Oral Health Prev Dent. 2020 Jul 4;18:455-460. doi: 10.3290/j.ohpd.a44683.