PMID- 32515437
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20210514
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 145
IP  - 14
DP  - 2020 Jul 21
TI  - Comparison of standard and HD FT-IR with multimodal CARS/TPEF/SHG/FLIMS imaging 
      in the detection of the early stage of pulmonary metastasis of murine breast 
      cancer.
PG  - 4982-4990
LID - 10.1039/d0an00762e [doi]
AB  - Lungs, due to their high oxygen availability and vascularization, are an ideal 
      environment for cancer cell migration, metastasis and tumour formation. These 
      processes are directly connected with extracellular matrix (ECM) remodelling, 
      resulting from cancer cell infiltration and preparation of the environment 
      suitable for tumour growth. Herein, we compare the potential of fast, label-free 
      and non-destructive methods of Fourier-transform infrared spectroscopy (FT-IR) in 
      standard and high definition (HD) modes with nonlinear coherent anti-Stokes Raman 
      scattering (CARS), second harmonic generation (SHG), two-photon excited 
      fluorescence (TPEF) and a fluorescence lifetime imaging (FLIM) technique for lung 
      metastasis detection. We show their potential in the detection of lung 
      macrometastasis, in which we already observed the ECM remodelling. The CARS image 
      revealed a dense cell fraction typical of ECM remodeling and reduction of the 
      TPEF signal together with an increase of fluorescence lifetime predominantly due 
      to NAD(P)H suggesting metabolic changes in the metastatic foci. FT-IR 
      spectroscopy allowed not only for macrometastasis detection but also their stage 
      definition based mainly on the analysis of proteins, RNA and glycogen fractions. 
      The multimodal approach additionally suggested partial enzymatic degradation of 
      elastin in ECM and collagen remodelling.
FAU - Chrabaszcz, Karolina
AU  - Chrabaszcz K
AD  - Faculty of Chemistry, Jagiellonian University, 2 Gronostajowa St., 30-387 Krakow, 
      Poland.
FAU - Meyer, Tobias
AU  - Meyer T
FAU - Bae, Hyeonsoo
AU  - Bae H
FAU - Schmitt, Michael
AU  - Schmitt M
FAU - Jasztal, Agnieszka
AU  - Jasztal A
FAU - Smeda, Marta
AU  - Smeda M
FAU - Stojak, Marta
AU  - Stojak M
FAU - Popp, Jurgen
AU  - Popp J
FAU - Malek, Kamilla
AU  - Malek K
FAU - Marzec, Katarzyna M
AU  - Marzec KM
LA  - eng
PT  - Journal Article
DEP - 20200609
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
SB  - IM
MH  - Animals
MH  - *Breast Neoplasms/diagnostic imaging
MH  - Humans
MH  - *Lung Neoplasms/diagnostic imaging
MH  - Mice
MH  - Photons
MH  - Spectroscopy, Fourier Transform Infrared
MH  - Spectrum Analysis, Raman
EDAT- 2020/06/10 06:00
MHDA- 2021/05/15 06:00
CRDT- 2020/06/10 06:00
PHST- 2020/06/10 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
PHST- 2020/06/10 06:00 [entrez]
AID - 10.1039/d0an00762e [doi]
PST - ppublish
SO  - Analyst. 2020 Jul 21;145(14):4982-4990. doi: 10.1039/d0an00762e. Epub 2020 Jun 9.