PMID- 32516488
OWN - NLM
STAT- MEDLINE
DCOM- 20210119
LR  - 20211002
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Print)
IS  - 0009-9104 (Linking)
VI  - 202
IP  - 1
DP  - 2020 Oct
TI  - Suppressor of cytokine signaling 1 inhibits the maturation of dendritic cells 
      involving the nuclear factor kappa B signaling pathway in the glioma 
      microenvironment.
PG  - 47-59
LID - 10.1111/cei.13476 [doi]
AB  - Recurrence and diffuse infiltration challenge traditional therapeutic strategies 
      for malignant glioma. Immunotherapy appears to be a promising approach to obtain 
      long-term survival. Dendritic cells (DCs), the most specialized and potent 
      antigen-presenting cells (APCs), play an important part in initiating and 
      amplifying both the innate and adaptive immune responses against cancer cells. 
      However, cancer cells can escape from immune surveillance by inhibiting 
      maturation of DCs. Until the present, molecular mechanisms of maturation 
      inhibition of DCs in the tumor microenvironment (TME) have not been fully 
      revealed. Our study showed that pretreatment with tumor-conditioned medium (TCM) 
      collected from supernatant of primary glioma cells significantly suppressed the 
      maturation of DCs. TCM pretreatment significantly changed the morphology of DCs, 
      TCM decreased the expression levels of CD80, CD83, CD86 and interleukin 
      (IL)-12p70, while it increased the expression levels of IL-10, transforming 
      growth factor (TGF)-beta and IL-6. RNA-Seq showed that TCM pretreatment 
      significantly increased the gene expression level of suppressor of cytokine 
      signaling 1 (SOCS1) in DCs. suppressor of cytokine signaling 1 (SOCS1) knock-down 
      significantly antagonized the maturation inhibition of DCs by TCM, which was 
      demonstrated by the restoration of maturation markers. TCM pretreatment also 
      significantly suppressed T cell viability and T helper type 1 (Th1) response, and 
      SOCS1 knock-down significantly antagonized this suppressive effect. Further, TCM 
      pretreatment significantly suppressed p65 nuclear translocation and 
      transcriptional activity in DCs, and SOCS1 knock-down significantly attenuated 
      this suppressive effect. In conclusion, our research demonstrates that TCM 
      up-regulate SOCS1 to suppress the maturation of DCs via the nuclear factor-kappa 
      signaling pathway.
CI  - (c) 2020 British Society for Immunology.
FAU - He, M
AU  - He M
AD  - Department of Neurosurgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen 
      University, Guangzhou, China.
AD  - Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene 
      Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 
      China.
FAU - Chen, X
AU  - Chen X
AD  - Department of Neurosurgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen 
      University, Guangzhou, China.
AD  - Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene 
      Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 
      China.
FAU - Luo, M
AU  - Luo M
AD  - Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene 
      Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 
      China.
AD  - Department of Oncology, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, 
      Guangzhou, China.
FAU - Ouyang, L
AU  - Ouyang L
AD  - Department of Neurosurgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen 
      University, Guangzhou, China.
AD  - Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene 
      Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 
      China.
FAU - Xie, L
AU  - Xie L
AD  - Department of Neurosurgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen 
      University, Guangzhou, China.
AD  - Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene 
      Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 
      China.
FAU - Huang, Z
AU  - Huang Z
AD  - Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene 
      Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 
      China.
AD  - Department of Neurosurgery, The Eighth Affiliated Hospital, Sun Yat-Sen 
      University, Shenzhen, China.
FAU - Liu, A
AU  - Liu A
AUID- ORCID: 0000-0002-7657-7266
AD  - Department of Neurosurgery, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen 
      University, Guangzhou, China.
AD  - Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene 
      Regulation, Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200630
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (NF-kappa B)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (SOCS1 protein, human)
RN  - 0 (Suppressor of Cytokine Signaling 1 Protein)
SB  - IM
MH  - Dendritic Cells/*immunology/pathology
MH  - Glioma/*immunology/pathology
MH  - Humans
MH  - NF-kappa B/*immunology
MH  - Neoplasm Proteins/*immunology
MH  - Signal Transduction/*immunology
MH  - Suppressor of Cytokine Signaling 1 Protein/*immunology
MH  - Th1 Cells/immunology/pathology
MH  - Tumor Cells, Cultured
MH  - Tumor Microenvironment/*immunology
PMC - PMC7488122
OTO - NOTNLM
OT  - NF-kappaB
OT  - SOCS1
OT  - dendritic cell
OT  - glioma
OT  - tumor microenvironment
COIS- None.
EDAT- 2020/06/10 06:00
MHDA- 2021/01/20 06:00
PMCR- 2021/10/01
CRDT- 2020/06/10 06:00
PHST- 2019/11/22 00:00 [received]
PHST- 2020/04/22 00:00 [revised]
PHST- 2020/05/24 00:00 [accepted]
PHST- 2020/06/10 06:00 [pubmed]
PHST- 2021/01/20 06:00 [medline]
PHST- 2020/06/10 06:00 [entrez]
PHST- 2021/10/01 00:00 [pmc-release]
AID - CEI13476 [pii]
AID - 10.1111/cei.13476 [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2020 Oct;202(1):47-59. doi: 10.1111/cei.13476. Epub 2020 Jun 
      30.