PMID- 32517217
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 2075-4418 (Print)
IS  - 2075-4418 (Electronic)
IS  - 2075-4418 (Linking)
VI  - 10
IP  - 6
DP  - 2020 Jun 6
TI  - A Fluorescence in Situ Hybridization (FISH) Test for Diagnosing Babesiosis.
LID - 10.3390/diagnostics10060377 [doi]
LID - 377
AB  - Apicomplexan parasites of the genus Babesia cause babesiosis in humans and 
      animals. The microscopic examination of stained blood smears, detection of serum 
      antibodies by immunoassays, and PCR-based identification of parasite nucleic acid 
      in blood are common laboratory methods for diagnosing babesiosis. The present 
      study evaluated a commercially available Babesia genus-specific fluorescence in 
      situ hybridization (FISH) test for detecting Babesia parasites in blood smears. 
      The FISH test detected Babesia duncani and Babesia microti, two common species 
      that cause human infections in the USA, and other Babesia species of human and 
      veterinary importance in less than two hours. The Babesia genus-specific FISH 
      test supplements other existing laboratory methods for diagnosing babesiosis and 
      may be particularly useful in resource-limited laboratories.
FAU - Shah, Jyotsna S
AU  - Shah JS
AD  - ID-FISH Technology Inc., 556 Gibraltar Drive, Milpitas, CA 95035, USA.
AD  - IGenex Inc., 556 Gibraltar Drive, Milpitas, CA 95035, USA.
FAU - Mark, Olivia
AU  - Mark O
AD  - IGenex Inc., 556 Gibraltar Drive, Milpitas, CA 95035, USA.
FAU - Caoili, Eddie
AU  - Caoili E
AD  - IGenex Inc., 556 Gibraltar Drive, Milpitas, CA 95035, USA.
FAU - Poruri, Akhila
AU  - Poruri A
AD  - IGenex Inc., 556 Gibraltar Drive, Milpitas, CA 95035, USA.
FAU - Horowitz, Richard I
AU  - Horowitz RI
AD  - Hudson Valley Healing Arts Center, New York, NY 12538, USA.
AD  - Member, HHS Subcommittee on Babesia and Tick-Borne Pathogens, US Department of 
      Health and Human Services, Washington, DC 20201, USA.
FAU - Ashbaugh, Alan D
AU  - Ashbaugh AD
AD  - College of Medicine, University of Cincinnati, Cincinnati, OH 45221, USA.
FAU - Ramasamy, Ranjan
AU  - Ramasamy R
AD  - ID-FISH Technology Inc., 556 Gibraltar Drive, Milpitas, CA 95035, USA.
LA  - eng
PT  - Journal Article
DEP - 20200606
PL  - Switzerland
TA  - Diagnostics (Basel)
JT  - Diagnostics (Basel, Switzerland)
JID - 101658402
PMC - PMC7344499
OTO - NOTNLM
OT  - Babesia
OT  - FISH
OT  - babesiosis
OT  - fluorescence in situ hybridization
OT  - laboratory diagnosis of babesiosis
COIS- J.S.S. owns stock and is chief executive of IGeneX and scientific director of 
      IDFISH; R.R.is an affiliate of IDFISH; O.M., E.C., and A.P. are employees at 
      IGeneX and IDFISH.
EDAT- 2020/06/11 06:00
MHDA- 2020/06/11 06:01
PMCR- 2020/06/06
CRDT- 2020/06/11 06:00
PHST- 2020/04/20 00:00 [received]
PHST- 2020/05/23 00:00 [revised]
PHST- 2020/06/02 00:00 [accepted]
PHST- 2020/06/11 06:00 [entrez]
PHST- 2020/06/11 06:00 [pubmed]
PHST- 2020/06/11 06:01 [medline]
PHST- 2020/06/06 00:00 [pmc-release]
AID - diagnostics10060377 [pii]
AID - diagnostics-10-00377 [pii]
AID - 10.3390/diagnostics10060377 [doi]
PST - epublish
SO  - Diagnostics (Basel). 2020 Jun 6;10(6):377. doi: 10.3390/diagnostics10060377.