PMID- 32517233
OWN - NLM
STAT- MEDLINE
DCOM- 20210301
LR  - 20210301
IS  - 2072-6643 (Electronic)
IS  - 2072-6643 (Linking)
VI  - 12
IP  - 6
DP  - 2020 Jun 6
TI  - Black Raspberries and Protocatechuic Acid Mitigate DNFB-Induced Contact 
      Hypersensitivity by Down-Regulating Dendritic Cell Activation and Inhibiting 
      Mediators of Effector Responses.
LID - 10.3390/nu12061701 [doi]
LID - 1701
AB  - Contact hypersensitivity (CHS) is the most common occupational dermatological 
      disease. Dendritic cells (DCs) mediate the sensitization stage of CHS, while 
      T-cells facilitate the effector mechanisms that drive CHS. Black raspberry (Rubus 
      occidentalis, BRB) and BRB phytochemicals possess immunomodulatory properties, 
      but their dietary effects on CHS are unknown. We examined the effects of diets 
      containing BRB and protocatechuic acid (PCA, a constituent of BRB and an 
      anthocyanin metabolite produced largely by gut microbes), on CHS, using a model 
      induced by 2,4-dinitrofluorobenze (DNFB). Mice were fed control diet or diets 
      supplemented with BRB or PCA. In vitro bone-marrow derived DCs and RAW264.7 
      macrophages were treated with BRB extract and PCA. Mice fed BRB or PCA 
      supplemented diets displayed decreased DNFB-induced ear swelling, marked by 
      decreased splenic DC accumulation. BRB extract diminished DC maturation 
      associated with reduced Cd80 expression and Interleukin (IL)-12 secretion, and 
      PCA reduced IL-12. Dietary supplementation with BRB and PCA induced differential 
      decreases in IL-12-driven CHS mediators, including Interferon (IFN)-gamma and IL-17 
      production by T-cells. BRB extracts and PCA directly attenuated CHS-promoting 
      macrophage activity mediated by nitric oxide and IL-12. Our results demonstrate 
      that BRB and PCA mitigate CHS pathology, providing a rationale for CHS 
      alleviation via dietary supplementation with BRB or BRB derived anthocyanins.
FAU - Anderson, Kelvin
AU  - Anderson K
AD  - Department of Pathology, The Ohio State University Wexner Medical Center, 
      Columbus, OH 43210, USA.
FAU - Ryan, Nathan
AU  - Ryan N
AD  - Department of Pathology, The Ohio State University Wexner Medical Center, 
      Columbus, OH 43210, USA.
AD  - Division of Anatomy, The Ohio State University Wexner Medical Center, Columbus, 
      OH 43210, USA.
FAU - Siddiqui, Arham
AU  - Siddiqui A
AD  - Department of Pathology, The Ohio State University Wexner Medical Center, 
      Columbus, OH 43210, USA.
FAU - Pero, Travis
AU  - Pero T
AD  - Department of Pathology, The Ohio State University Wexner Medical Center, 
      Columbus, OH 43210, USA.
AD  - College of Dentistry, The Ohio State University, Columbus, OH 43210, USA.
FAU - Volpedo, Greta
AU  - Volpedo G
AUID- ORCID: 0000-0002-1112-7715
AD  - Department of Pathology, The Ohio State University Wexner Medical Center, 
      Columbus, OH 43210, USA.
AD  - Department of Microbiology, The Ohio State University, Columbus, OH 43210, USA.
FAU - Cooperstone, Jessica L
AU  - Cooperstone JL
AD  - Department of Horticulture and Crop Science, The Ohio State University, Columbus, 
      OH 43210, USA.
AD  - Department of Food Science and Technology, The Ohio State University, Columbus, 
      OH 43210, USA.
FAU - Oghumu, Steve
AU  - Oghumu S
AUID- ORCID: 0000-0001-5177-0652
AD  - Department of Pathology, The Ohio State University Wexner Medical Center, 
      Columbus, OH 43210, USA.
LA  - eng
GR  - K01 CA207599/CA/NCI NIH HHS/United States
GR  - K01CA207599/CA/NCI NIH HHS/United States
GR  - Seed Grant/Foods for Health (FFH) Discovery Theme & Food Innovation Center (FIC)/
PT  - Journal Article
DEP - 20200606
PL  - Switzerland
TA  - Nutrients
JT  - Nutrients
JID - 101521595
RN  - 0 (B7-1 Antigen)
RN  - 0 (Hydroxybenzoates)
RN  - 0 (Interleukin-17)
RN  - 0 (Plant Extracts)
RN  - 187348-17-0 (Interleukin-12)
RN  - 36R5QJ8L4B (protocatechuic acid)
RN  - 82115-62-6 (Interferon-gamma)
RN  - D241E059U6 (Dinitrofluorobenzene)
SB  - IM
MH  - Animals
MH  - B7-1 Antigen/metabolism
MH  - Dendritic Cells/*immunology
MH  - Dermatitis, Contact/etiology/*immunology/metabolism/*therapy
MH  - *Dietary Supplements
MH  - Dinitrofluorobenzene/*adverse effects
MH  - Disease Models, Animal
MH  - Hydroxybenzoates/*pharmacology/*therapeutic use
MH  - Interferon-gamma/metabolism
MH  - Interleukin-12/metabolism
MH  - Interleukin-17/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - *Phytotherapy
MH  - Plant Extracts/*pharmacology/*therapeutic use
MH  - RAW 264.7 Cells
MH  - *Rubus
MH  - T-Lymphocytes/immunology
PMC - PMC7352349
OTO - NOTNLM
OT  - IFN-gamma
OT  - Rubus occidentalis
OT  - contact hypersensitivity
OT  - dendritic cells
OT  - inflammatory
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; in 
      the writing of the manuscript, or in the decision to publish the results.
EDAT- 2020/06/11 06:00
MHDA- 2021/03/02 06:00
PMCR- 2020/06/01
CRDT- 2020/06/11 06:00
PHST- 2020/05/24 00:00 [received]
PHST- 2020/06/04 00:00 [accepted]
PHST- 2020/06/11 06:00 [entrez]
PHST- 2020/06/11 06:00 [pubmed]
PHST- 2021/03/02 06:00 [medline]
PHST- 2020/06/01 00:00 [pmc-release]
AID - nu12061701 [pii]
AID - nutrients-12-01701 [pii]
AID - 10.3390/nu12061701 [doi]
PST - epublish
SO  - Nutrients. 2020 Jun 6;12(6):1701. doi: 10.3390/nu12061701.