PMID- 32518048
OWN - NLM
STAT- MEDLINE
DCOM- 20210830
LR  - 20210830
IS  - 1286-0115 (Print)
IS  - 1286-0115 (Linking)
VI  - 104
IP  - 346
DP  - 2020 Sep
TI  - Biomaterials preparation by electrospinning of gelatin and sodium 
      hyaluronate/gelatin nanofibers with non-toxic solvents.
PG  - 158-168
LID - S1286-0115(20)30044-8 [pii]
LID - 10.1016/j.morpho.2020.05.002 [doi]
AB  - Gelatin (Ge) based fibers have been produced by electrospinning with a non-toxic 
      solvent for preparing membranes usable in maxillofacial surgery. Ge and Ge/sodium 
      hyaluronate (SH) nanofibers were successfully electrospun to produce membranes 
      whose thickness was around 150 to 200mum. The mean fiber diameter reached a 
      maximum of 660nm for Ge fibers and 210nm for Ge/SH fibers. The presence of Ge and 
      SH was confirmed in the membranes by Raman spectroscopy. Ge membranes had low 
      mechanical properties and only small samples of 0.5cm in size could be retrieved 
      from the collector as larger sample tended to tear and break. Ge/SH membranes 
      could be retrieved from the collector slightly easily. Membranes could be handled 
      carefully but in vivo implantation could not be planned due to poor mechanical 
      resistance. Crosslinking by glutaraldehyde vapors reduced the mean porosity of Ge 
      membranes; it totally prevents membranes to be retrieved from the collector. Beta 
      tricalcium phosphate (beta-TCP) particles were added with Ge during electrospinning 
      to increase osseointegration of the membranes and promote bone formation. beta-TCP 
      particles formed agglomerates outside the fibers, and we could not obtain beta-TCP 
      particles inside the Ge fibers due to their low diameter. In general, electrospun 
      membranes lacked reproducibility. Despite the great interest of Ge-based 
      membranes and Ge/beta-TCP membranes, the low mechanical properties of the fibers, 
      the lack of reproducibility and the difficulty to retrieve the membranes from the 
      collector did not allow our biomaterials to be implanted or to be envisaged for 
      industrial production.
CI  - Copyright (c) 2020. Published by Elsevier Masson SAS.
FAU - Arbez, B
AU  - Arbez B
AD  - Groupe Etudes Remodelage Osseux et bioMateriaux (GEROM), LabCom NextBone, 
      IRIS-IBS institut de biologie en sante, universite d'Angers, CHU d'Angers, 49933 
      Angers cedex, France.
FAU - Libouban, H
AU  - Libouban H
AD  - Groupe Etudes Remodelage Osseux et bioMateriaux (GEROM), LabCom NextBone, 
      IRIS-IBS institut de biologie en sante, universite d'Angers, CHU d'Angers, 49933 
      Angers cedex, France. Electronic address: helene.libouban@univ-angers.fr.
LA  - eng
PT  - Journal Article
DEP - 20200606
PL  - France
TA  - Morphologie
JT  - Morphologie : bulletin de l'Association des anatomistes
JID - 9814314
RN  - 0 (Biocompatible Materials)
RN  - 0 (Solvents)
RN  - 9000-70-8 (Gelatin)
RN  - 9004-61-9 (Hyaluronic Acid)
SB  - IM
MH  - *Biocompatible Materials
MH  - Gelatin
MH  - Hyaluronic Acid
MH  - *Nanofibers
MH  - Reproducibility of Results
MH  - Solvents
OTO - NOTNLM
OT  - Electrospinning
OT  - Gelatin
OT  - Hyaluronic acid
OT  - Nanofibers
OT  - Raman spectroscopy
OT  - SEM
OT  - beta-TCP
EDAT- 2020/06/11 06:00
MHDA- 2021/08/31 06:00
CRDT- 2020/06/11 06:00
PHST- 2020/04/02 00:00 [received]
PHST- 2020/05/14 00:00 [accepted]
PHST- 2020/06/11 06:00 [pubmed]
PHST- 2021/08/31 06:00 [medline]
PHST- 2020/06/11 06:00 [entrez]
AID - S1286-0115(20)30044-8 [pii]
AID - 10.1016/j.morpho.2020.05.002 [doi]
PST - ppublish
SO  - Morphologie. 2020 Sep;104(346):158-168. doi: 10.1016/j.morpho.2020.05.002. Epub 
      2020 Jun 6.