PMID- 32520222
OWN - NLM
STAT- MEDLINE
DCOM- 20200730
LR  - 20220629
IS  - 1980-5322 (Electronic)
IS  - 1807-5932 (Print)
IS  - 1807-5932 (Linking)
VI  - 75
DP  - 2020
TI  - Effects and mechanism of stem cells from human exfoliated deciduous teeth 
      combined with hyperbaric oxygen therapy in type 2 diabetic rats.
PG  - e1656
LID - 10.6061/clinics/2020/e1656 [doi]
LID - e1656
AB  - OBJECTIVES: Mesenchymal stem cells (MSCs) are potentially ideal for type 2 
      diabetes treatment, owing to their multidirectional differentiation ability and 
      immunomodulatory properties. Here we investigated whether the stem cells from 
      human exfoliated deciduous teeth (SHED) in combination with hyperbaric oxygen 
      (HBO) could treat type 2 diabetic rats, and explored the underlying mechanism. 
      METHODS: SD rats were used to generate a type 2 diabetes model, which received 
      stem cell therapy, HBO therapy, or both together. Before and after treatment, 
      body weight, blood glucose, and serum insulin, blood lipid, pro-inflammatory 
      cytokines (tumor necrosis factor-alpha and interleukin-6), and urinary proteins 
      were measured and compared. After 6 weeks, rats were sacrificed and their organs 
      were subjected to hematoxylin and eosin staining and immunofluorescence staining 
      for insulin and glucagon; apoptosis and proliferation were analyzed in islet 
      cells. Structural changes in islets were observed under an electron microscope. 
      Expression levels of Pdx1, Ngn3, and Pax4 mRNAs in the pancreas were assessed by 
      real-time quantitative polymerase chain reaction (RT-qPCR). RESULTS: In 
      comparison with diabetic mice, those treated with the combination or SHE therapy 
      showed decreased blood glucose, insulin resistance, serum lipids, and 
      pro-inflammatory cytokines and increased body weight and serum insulin. The 
      morphology and structure of pancreatic islets improved, as evident from an 
      increase in insulin-positive cells and a decrease in glucagon-positive cells. 
      Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) 
      staining of islet cells revealed the decreased apoptosis index, while Ki67 and 
      proliferating cell nuclear antigen staining showed increased proliferation index. 
      Pancreatic expression of Pdx1, Ngn3, and Pax4 was upregulated. CONCLUSION: SHED 
      combined with HBO therapy was effective for treating type 2 diabetic rats. The 
      underlying mechanism may involve SHED-mediated increase in the proliferation and 
      trans-differentiation of islet beta-cells and decrease in pro-inflammatory cytokines 
      and apoptosis of islets.
FAU - Xu, Yifeng
AU  - Xu Y
AUID- ORCID: 0000-0001-9258-2690
AD  - Department of Endocrinology, Changhai Hospital, the First Affiliated Hospital of 
      the Naval Medical University, Shanghai 200433, China.
AD  - Department of Endocrinology, Air Force Hospital of Northern Theater Command of 
      PLA, Shenyang 110042, China.
FAU - Chen, Jin
AU  - Chen J
AD  - Department of Endocrinology, Changhai Hospital, the First Affiliated Hospital of 
      the Naval Medical University, Shanghai 200433, China.
FAU - Zhou, Hui
AU  - Zhou H
AD  - Department of Out-patient, Changning retired cadre retreat of Shanghai garrison 
      command, Shanghai 200050, China.
FAU - Wang, Jing
AU  - Wang J
AD  - Department of Endocrinology, Changhai Hospital, the First Affiliated Hospital of 
      the Naval Medical University, Shanghai 200433, China.
AD  - Department of Internal Medcine, Hotan Country People's Hospital of Xinjiang, 
      Hotan Country 848000, China.
FAU - Song, Jingyun
AU  - Song J
AD  - Department of Endocrinology, Changhai Hospital, the First Affiliated Hospital of 
      the Naval Medical University, Shanghai 200433, China.
FAU - Xie, Junhao
AU  - Xie J
AD  - Department of Endocrinology, Changhai Hospital, the First Affiliated Hospital of 
      the Naval Medical University, Shanghai 200433, China.
FAU - Guo, Qingjun
AU  - Guo Q
AD  - Department of Endocrinology, Changhai Hospital, the First Affiliated Hospital of 
      the Naval Medical University, Shanghai 200433, China.
FAU - Wang, Chaoqun
AU  - Wang C
AD  - Department of Endocrinology, Changhai Hospital, the First Affiliated Hospital of 
      the Naval Medical University, Shanghai 200433, China.
FAU - Huang, Qin
AU  - Huang Q
AUID- ORCID: 0000-0002-8678-4277
AD  - Department of Endocrinology, Changhai Hospital, the First Affiliated Hospital of 
      the Naval Medical University, Shanghai 200433, China.
LA  - eng
PT  - Journal Article
DEP - 20200603
PL  - United States
TA  - Clinics (Sao Paulo)
JT  - Clinics (Sao Paulo, Brazil)
JID - 101244734
RN  - 0 (Insulin)
SB  - IM
MH  - Animals
MH  - China
MH  - Diabetes Mellitus, Experimental/*therapy
MH  - Diabetes Mellitus, Type 2/chemically induced/*therapy
MH  - Humans
MH  - Hyperbaric Oxygenation/*methods
MH  - Insulin
MH  - *Insulin-Secreting Cells
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells
MH  - Mice
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Stem Cells
MH  - Tooth, Deciduous
PMC - PMC7247751
COIS- No potential conflict of interest was reported.
EDAT- 2020/06/11 06:00
MHDA- 2020/07/31 06:00
PMCR- 2020/01/01
CRDT- 2020/06/11 06:00
PHST- 2019/11/28 00:00 [received]
PHST- 2020/02/10 00:00 [accepted]
PHST- 2020/06/11 06:00 [entrez]
PHST- 2020/06/11 06:00 [pubmed]
PHST- 2020/07/31 06:00 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - S1807-5932(22)00328-3 [pii]
AID - cln_75p1 [pii]
AID - 10.6061/clinics/2020/e1656 [doi]
PST - epublish
SO  - Clinics (Sao Paulo). 2020 Jun 3;75:e1656. doi: 10.6061/clinics/2020/e1656. 
      eCollection 2020.