PMID- 32521582
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 2233-8233 (Print)
IS  - 2233-8241 (Electronic)
IS  - 2093-8896 (Linking)
VI  - 47
IP  - 2
DP  - 2020 Jun
TI  - Effects of acrylamide in the presence of vitamin E on sperm parameters, chromatin 
      quality, and testosterone levels in mice.
PG  - 101-107
LID - 10.5653/cerm.2019.03230 [doi]
AB  - OBJECTIVE: The present study investigated sperm chromatin quality and 
      testosterone levels in acrylamide-treated mice and the possible protective 
      effects of vitamin E on the fertility potential of spermatozoa. METHODS: 
      Thirty-two adult male mice were divided equally into four groups. Group 1 was the 
      control, group 2 received acrylamide (10 mg/kg, water solution), group 3 received 
      vitamin E (100 mg/kg, intraperitoneal), and group 4 received both acrylamide and 
      vitamin E. After 35 days, spermatozoa from the right cauda epididymis were 
      analyzed in terms of count, motility, morphology, and viability. Sperm DNA 
      integrity and chromatin condensation were assessed by acridine orange (AO), 
      aniline blue (AB), toluidine blue (TB), and chromomycin A3 (CMA3) staining. 
      RESULTS: In acrylamide-treated mice, significantly lower sperm concentration, 
      viability, motility, and testosterone levels were found in comparison with the 
      control and acrylamide+vitamin E groups (p<0.05). In the vitamin E group, 
      significantly more favorable sperm parameters and testosterone levels were found 
      than in the other groups (p<0.05). There were also significantly more spermatozoa 
      with less condensed chromatin in the acrylamide-treated mice than in the other 
      groups. Moreover, significantly more spermatozoa with mature nuclei (assessed by 
      AB, CMA3, AO, and TB staining) were present in the vitamin E group than in the 
      control and acrylamide+vitamin E groups. CONCLUSION: This study revealed the 
      deleterious effects of acrylamide on sperm parameters and sperm chromatin 
      quality. Vitamin E can not only compensate for the toxic effects of acrylamide, 
      but also improve sperm chromatin quality in mice.
FAU - Anvari, Morteza
AU  - Anvari M
AD  - Department of Biology and Anatomical Sciences, Shahid Sadoughi University of 
      Medical Sciences, Yazd, Iran.
AD  - Research and Clinical Center for Infertility, Shahid Sadoughi University of 
      Medical Sciences, Yazd, Iran.
FAU - Talebi, Ali Reza
AU  - Talebi AR
AD  - Department of Biology and Anatomical Sciences, Shahid Sadoughi University of 
      Medical Sciences, Yazd, Iran.
AD  - Research and Clinical Center for Infertility, Shahid Sadoughi University of 
      Medical Sciences, Yazd, Iran.
FAU - Mangoli, Esmat
AU  - Mangoli E
AD  - Research and Clinical Center for Infertility, Shahid Sadoughi University of 
      Medical Sciences, Yazd, Iran.
FAU - Shahedi, Abbas
AU  - Shahedi A
AD  - Department of Biology and Anatomical Sciences, Shahid Sadoughi University of 
      Medical Sciences, Yazd, Iran.
FAU - Ghasemi, Mohammad Rasool
AU  - Ghasemi MR
AD  - Department of Biology and Anatomical Sciences, Shahid Sadoughi University of 
      Medical Sciences, Yazd, Iran.
FAU - Pourentezari, Majid
AU  - Pourentezari M
AD  - Department of Biology and Anatomical Sciences, Shahid Sadoughi University of 
      Medical Sciences, Yazd, Iran.
LA  - eng
PT  - Journal Article
DEP - 20200601
PL  - Korea (South)
TA  - Clin Exp Reprod Med
JT  - Clinical and experimental reproductive medicine
JID - 101563916
PMC - PMC7315862
OTO - NOTNLM
OT  - Acrylamide
OT  - Chromatin
OT  - Mice
OT  - Spermatozoa
OT  - Testosterone
OT  - Vitamin E
COIS- Conflict of interest No potential conflict of interest relevant to this article 
      was reported.
EDAT- 2020/06/11 06:00
MHDA- 2020/06/11 06:01
PMCR- 2020/06/01
CRDT- 2020/06/11 06:00
PHST- 2019/08/11 00:00 [received]
PHST- 2019/11/03 00:00 [accepted]
PHST- 2020/06/11 06:00 [entrez]
PHST- 2020/06/11 06:00 [pubmed]
PHST- 2020/06/11 06:01 [medline]
PHST- 2020/06/01 00:00 [pmc-release]
AID - cerm.2019.03230 [pii]
AID - cerm-2019-03230 [pii]
AID - 10.5653/cerm.2019.03230 [doi]
PST - ppublish
SO  - Clin Exp Reprod Med. 2020 Jun;47(2):101-107. doi: 10.5653/cerm.2019.03230. Epub 
      2020 Jun 1.