PMID- 32521878
OWN - NLM
STAT- MEDLINE
DCOM- 20210208
LR  - 20210208
IS  - 2241-6293 (Electronic)
IS  - 1107-0625 (Linking)
VI  - 25
IP  - 2
DP  - 2020 Mar-Apr
TI  - Antiproliferative effects of Norartocarpetin isoflavone in human lung carcinoma 
      cells are mediated via targeting Ras/Raf/MAPK signalling pathway, mitochondrial 
      mediated apoptosis, S-phase cell cycle arrest and suppression of cell migration 
      and invasion.
PG  - 855-861
AB  - PURPOSE: The main purpose of the current research work was to investigate the 
      anticancer activity of norartocarpetin - a plant derived isoflavone -in human 
      lung carcinoma cells NCI-H460 and normal lung cell line MRC-9 along with studying 
      its effects on cellular apoptosis, DNA damage, cell migration and invasion and 
      Ras/Raf/MAPK signalling pathways. METHODS: Cell proliferation was examined by 
      CCK-8 assay while effects on apoptosis were evaluated by acridine orange 
      (AO)/ethidium bormide (EB) staining and Comet assay using fluorescence 
      microscopy. In vitro wound healing assay was used for checking the effects on 
      cell migration and transwell assay for invasion while western blot was used to 
      evaluate the effects on the expression of Ras/Raf/MAPK proteins. RESULTS: The 
      results showed that Norartocarpetin led to dose-dependent cytotoxic effects in 
      NCI-H460 cells showing an IC50 value of 22 muM while in normal lung cells, the 
      cytotoxic effects were much lower as shown by higher IC50 value of 85 muM. It also 
      led to dose-dependent apoptosis and induced DNA damage as shown by fluorescence 
      microscopy. This molecule also inhibited cell migration and invasion 
      dose-dependently, along with inhibiting MMP-9 expression. Norartocarpetin 
      treatment also led to inhibition of the expression of Ras/Raf/MAPK proteins and 
      also caused S-phase cell cycle arrest in these cells. CONCLUSION: Norartocarpetin 
      has a significant anticancer activity in lung carcinoma cells and these effects 
      are mediated via targeting apoptosis, DNA damage, cell migration and invasion, 
      cell cycle and inhibiting Ras/Raf/MAPK signalling pathways.
FAU - Guo, Ning
AU  - Guo N
AD  - Department of Cardio Thoracic Surgery, Puren Hospital of Wuhan University of 
      Science and Technology, Wuhan 430081, China.
FAU - Xiao, Yuanyang
AU  - Xiao Y
FAU - Chen, Daxing
AU  - Chen D
FAU - Wang, Jie
AU  - Wang J
LA  - eng
PT  - Journal Article
PL  - Cyprus
TA  - J BUON
JT  - Journal of B.U.ON. : official journal of the Balkan Union of Oncology
JID - 100883428
RN  - 0 (Isoflavones)
SB  - IM
MH  - Apoptosis
MH  - Cell Cycle Checkpoints/*drug effects
MH  - Cell Movement/*drug effects
MH  - Cell Proliferation
MH  - Flow Cytometry
MH  - Humans
MH  - Isoflavones/pharmacology/*therapeutic use
MH  - Lung Neoplasms/*drug therapy/pathology
MH  - MAP Kinase Signaling System/*drug effects
MH  - Neoplasm Invasiveness
MH  - Signal Transduction
EDAT- 2020/06/12 06:00
MHDA- 2021/02/09 06:00
CRDT- 2020/06/12 06:00
PHST- 2020/06/12 06:00 [entrez]
PHST- 2020/06/12 06:00 [pubmed]
PHST- 2021/02/09 06:00 [medline]
PST - ppublish
SO  - J BUON. 2020 Mar-Apr;25(2):855-861.