PMID- 32521880
OWN - NLM
STAT- MEDLINE
DCOM- 20210208
LR  - 20210208
IS  - 2241-6293 (Electronic)
IS  - 1107-0625 (Linking)
VI  - 25
IP  - 2
DP  - 2020 Mar-Apr
TI  - Herbal extract of Artemisia maritima induces selective anti-tumor effects in 
      three human lung carcinoma cells (H1299, NCI-H1437, PC-14) through activating 
      apoptosis-related proteins, G2/M cell cycle arrest and suppression of cell 
      migration.
PG  - 869-874
AB  - PURPOSE: Non-small cell lung cancer (NSCLC) is the leading cause of 
      cancer-related deaths in the United States, accounting for 85% of all diagnosed 
      lung cancers and resulting in over 100,000 deaths per year. The main purpose of 
      the current study was to investigate the antiproliferative effects of ethanolic 
      extract of Artemisia maritima in three human lung cancer cell lines along with 
      studying the effects of the herbal extract on cellular apoptosis, G2/M cell cycle 
      arrest and cell migration. METHODS: The CCK-8 assay was used to determine cell 
      viability. Apoptosis was detected by using acridine orange (AO)/ethidium bromide 
      (EB) staining, annexin V/propidium iodide (PI) assay and western blot. The cell 
      migration was determined by wound healing assay while the effects on cell cycle 
      were evaluated by flow cytometry. RESULTS: The results showed that herbal extract 
      of Artemisia maritima decreased the viability of all three cell lines H1299, 
      NCI-H1437, PC-14 dose-dependently with maximum effect on NCI-H1437 cell line. The 
      antiproliferative effects were due to the activation of mitochondrial-dependent 
      apoptotic pathway as seen by fluorescence microscopy which showed chromatin 
      condensation and nuclear fragmentation. This was also associated with increase in 
      Bax and decrease in Bcl-2 levels. Artemisia maritima extract treatment also led 
      to G2/M phase cell cycle arrest along with strong inhibition of cell migration. 
      CONCLUSIONS: In conclusion, the results of the current study clearly indicate 
      that Artemisia maritima extract exhibits antiproliferative effects in Nonsmall 
      cell lung cancer cells by triggering apoptosis, cell cycle arrest and inhibition 
      of cell migration.
FAU - Tang, Junqi
AU  - Tang J
AD  - Department of Respiratory Medicine, Hospital of Traditional Chinese Medicine, 
      Affiliated to Southwest Medical University, Luzhou 646000, China.
FAU - Guo, Zhihong
AU  - Guo Z
FAU - Wen, Yan
AU  - Wen Y
FAU - Ao, Suhua
AU  - Ao S
FAU - Wang, Junfeng
AU  - Wang J
LA  - eng
PT  - Journal Article
PL  - Cyprus
TA  - J BUON
JT  - Journal of B.U.ON. : official journal of the Balkan Union of Oncology
JID - 100883428
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Artemisia/*chemistry
MH  - Cell Cycle Checkpoints/*drug effects
MH  - Cell Line, Tumor
MH  - Cell Movement/*drug effects
MH  - Herbal Medicine/*methods
MH  - Humans
MH  - Lung Neoplasms/*drug therapy/pathology
EDAT- 2020/06/12 06:00
MHDA- 2021/02/09 06:00
CRDT- 2020/06/12 06:00
PHST- 2020/06/12 06:00 [entrez]
PHST- 2020/06/12 06:00 [pubmed]
PHST- 2021/02/09 06:00 [medline]
PST - ppublish
SO  - J BUON. 2020 Mar-Apr;25(2):869-874.