PMID- 32521882
OWN - NLM
STAT- MEDLINE
DCOM- 20210208
LR  - 20210208
IS  - 2241-6293 (Electronic)
IS  - 1107-0625 (Linking)
VI  - 25
IP  - 2
DP  - 2020 Mar-Apr
TI  - Naturally occurring triterpene Lupane exerts anticancer effects on colorectal 
      cancer cells via induction of apoptosis and autophagy and suppresses cell 
      migration and invasion by targeting MMP-9.
PG  - 884-889
AB  - PURPOSE: This study was undertaken with a purpose to examine the anticancer 
      effects of Lupane against human colorectal cancer. METHODS: The SW48 colorectal 
      cell line and CDD18Co normal colon cell line were used in this study. The CCK8 
      assay was used to determine cell proliferation while acridine orange 
      (AO)/ethidium bromide (EB) and DAPI staining assays were used to detect 
      apoptosis. Wound healing and transwell assays were used to detect the cell 
      migration and invasion. Western blotting was used to determine protein 
      expression. RESULTS: Lupane inhibited the proliferation of colorectal cancer 
      cells and the level of inhibition followed dose-dependent pattern. The 
      antiproliferative role of Lupane was exerted via induction of apoptotic cell 
      death. Western blot showed that the expression of Bcl-2 was decreased and that of 
      Bax was increaced. Lupane also prompted the autophagy of the SW48 colorectal 
      cancer cells and enhanced the expression of LC3-II. However, the expression of 
      p62 was depleted. The treatment of Lupane also resulted to inhibition of the 
      migratory potential of cancer cells as revealed by the wound healing assay. The 
      invasion of SW48 cancer cells was also suppressed and was associated with 
      suppression of metalloproteinase-9 (MMP-9) expression. CONCLUSION: The results 
      indicate the anticancer potential of Lupane against the colorectal cancer growth 
      and propagation. The study envisages the importance of natural compounds for 
      their usage against human cancers.
FAU - Zhou, Qiming
AU  - Zhou Q
AD  - Cancer Center, Huazhong University of Science and Technology Union, Shenzhen 
      Hospital, Nanshan District, Shenzhen 518052, China.
FAU - Zhao, Yan
AU  - Zhao Y
FAU - Fu, Xiaohong
AU  - Fu X
FAU - Chen, Qianqi
AU  - Chen Q
FAU - Tang, Yueqiang
AU  - Tang Y
FAU - Gao, Xiaoping
AU  - Gao X
LA  - eng
PT  - Journal Article
PL  - Cyprus
TA  - J BUON
JT  - Journal of B.U.ON. : official journal of the Balkan Union of Oncology
JID - 100883428
RN  - 0 (Triterpenes)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Autophagy/*drug effects
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Colorectal Neoplasms/*drug therapy/genetics
MH  - Flow Cytometry
MH  - Humans
MH  - Matrix Metalloproteinase 9/*metabolism
MH  - Triterpenes/pharmacology/*therapeutic use
EDAT- 2020/06/12 06:00
MHDA- 2021/02/09 06:00
CRDT- 2020/06/12 06:00
PHST- 2020/06/12 06:00 [entrez]
PHST- 2020/06/12 06:00 [pubmed]
PHST- 2021/02/09 06:00 [medline]
PST - ppublish
SO  - J BUON. 2020 Mar-Apr;25(2):884-889.