PMID- 32525071
OWN - NLM
STAT- MEDLINE
DCOM- 20210329
LR  - 20210329
IS  - 1873-6351 (Electronic)
IS  - 0278-6915 (Linking)
VI  - 142
DP  - 2020 Aug
TI  - Taxifolin attenuates the developmental testicular toxicity induced by di-n-butyl 
      phthalate in fetal male rats.
PG  - 111482
LID - S0278-6915(20)30372-0 [pii]
LID - 10.1016/j.fct.2020.111482 [doi]
AB  - Di-n-butyl phthalate (DBP) is widely used in consumer products as a plasticizer. 
      Here, we report a natural product taxifolin that can attenuate developmental and 
      reproductive toxicity of DBP. Pregnant rats were daily gavaged with 500 mg/kg DBP 
      alone or together with taxifolin (10 and 20 mg/kg) from gestational day (GD) 
      12-21. At GD21, sera and testes of male fetus were collected. DBP significantly 
      lowered serum testosterone level at 500 mg/kg and taxifolin can completely 
      reverse its action. DBP caused abnormal aggregation of fetal Leydig cells and 
      taxifolin can reverse it. DBP down-regulated the expression of the genes of 
      cholesterol side-chain cleavage enzyme (Cyp11a1), 17beta-hydroxysteroid 
      dehydrogenase 3 (Hsd17b3), and insulin-like 3 (Insl3) and taxifolin can reverse 
      its action. DBP increased malondialdehyde levels and decreased superoxide 
      dismutase and glutathione peroxidase expression and taxifolin can reverse it. DBP 
      increased incidence of multinucleated gonocytes and taxifolin can prevent it. 
      Moreover, DBP lowered sirtuin 1 (SIRT1)/peroxisome proliferator-activated 
      receptor gamma coactivator 1-alpha (PGC-1alpha) and phosphorylated AMP-activated protein 
      kinase (pAMPK) signalling and taxifolin antagonized DBP. In conclusion, in utero 
      exposure to DBP caused developmental/reproductive toxicity of male offspring via 
      increasing reactive oxygen species and taxifolin is an effective food component 
      that completely reverses DBP-mediated action.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Li, Zengqiang
AU  - Li Z
AD  - Department of Anesthesiology, The Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, 
      People's Republic of China.
FAU - Yu, Yige
AU  - Yu Y
AD  - Department of Anesthesiology, The Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, 
      People's Republic of China.
FAU - Li, Yang
AU  - Li Y
AD  - Department of Anesthesiology, The Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, 
      People's Republic of China.
FAU - Ma, Feifei
AU  - Ma F
AD  - Department of Anesthesiology, The Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, 
      People's Republic of China.
FAU - Fang, Yinghui
AU  - Fang Y
AD  - Department of Anesthesiology, The Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, 
      People's Republic of China.
FAU - Ni, Chaobo
AU  - Ni C
AD  - Department of Anesthesiology, The Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, 
      People's Republic of China.
FAU - Wu, Keyang
AU  - Wu K
AD  - Department of Anesthesiology, The Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, 
      People's Republic of China.
FAU - Pan, Peipei
AU  - Pan P
AD  - Department of Anesthesiology, The Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, 
      People's Republic of China.
FAU - Ge, Ren-Shan
AU  - Ge RS
AD  - Department of Anesthesiology, The Second Affiliated Hospital and Yuying 
      Children's Hospital of Wenzhou Medical University, Wenzhou, Zhejiang, 325027, 
      People's Republic of China. Electronic address: r_ge@yahoo.com.
LA  - eng
PT  - Journal Article
DEP - 20200607
PL  - England
TA  - Food Chem Toxicol
JT  - Food and chemical toxicology : an international journal published for the British 
      Industrial Biological Research Association
JID - 8207483
RN  - 0 (Antioxidants)
RN  - 0 (Plasticizers)
RN  - 2286E5R2KE (Dibutyl Phthalate)
RN  - 9IKM0I5T1E (Quercetin)
RN  - 9SOB9E3987 (taxifolin)
SB  - IM
MH  - Animals
MH  - Antioxidants/*pharmacology
MH  - Dibutyl Phthalate/*toxicity
MH  - Female
MH  - Male
MH  - Maternal Exposure
MH  - Plasticizers/*toxicity
MH  - Quercetin/*analogs & derivatives/pharmacology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Testis/*drug effects/embryology
OTO - NOTNLM
OT  - Fetal Leydig cell
OT  - Fetal Leydig cell aggregation
OT  - Oxidative stress
OT  - Taxifolin
OT  - Testosterone
OT  - di-n-butyl phthalate
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2020/06/12 06:00
MHDA- 2021/03/30 06:00
CRDT- 2020/06/12 06:00
PHST- 2019/12/21 00:00 [received]
PHST- 2020/05/12 00:00 [revised]
PHST- 2020/05/28 00:00 [accepted]
PHST- 2020/06/12 06:00 [pubmed]
PHST- 2021/03/30 06:00 [medline]
PHST- 2020/06/12 06:00 [entrez]
AID - S0278-6915(20)30372-0 [pii]
AID - 10.1016/j.fct.2020.111482 [doi]
PST - ppublish
SO  - Food Chem Toxicol. 2020 Aug;142:111482. doi: 10.1016/j.fct.2020.111482. Epub 2020 
      Jun 7.