PMID- 32525822
OWN - NLM
STAT- MEDLINE
DCOM- 20200911
LR  - 20211204
IS  - 0231-5882 (Print)
IS  - 0231-5882 (Linking)
VI  - 39
IP  - 3
DP  - 2020 May
TI  - Angiopoietin-like protein 7 mediates TNF-alpha-induced adhesion and oxidative stress 
      in human umbilical vein epithelial cell.
PG  - 285-292
LID - 10.4149/gpb_2019062 [doi]
AB  - Tumor necrosis factor-alpha (TNF-alpha) promotes monocyte adhesion to endothelium and 
      accumulation of endothelium will lead to atherosclerosis. The present study 
      explored Angiopoietin-like protein (Angptl7) as a potential target in the process 
      of atherosclerosis, and its role in the adhesion and oxidative stress induced by 
      TNF-alpha in human umbilical vein epithelial cells (HUVEC). The initiation of 
      atherosclerosis is endothelial injury. Angptl7 was dramatically increased in 
      TNF-alpha-induced HUVEC compared to the control cells. After Angptl7 effectively 
      knocked-down in TNF-alpha-induced HUVEC, the levels of reactive oxygen species (ROS), 
      interleukin (IL)-1beta, IL-6 and cyclooxygenase-2 (Cox-2) were prominently 
      decreased, whereas the levels of nitric oxide (NO) and endothelia nitric oxide 
      synthase (eNOS) were increased. Inhibition of Angptl7 significantly reversed 
      TNF-alpha-induced cell adhesion in HUVEC. Finally, downregulation of Angptl7 
      significantly reduced the expression of nuclear factor-kappaB (NF-kappaB) and enhanced 
      the levels of nuclear factor erythroid 2-related factor 2 (Nrf-2) and heme 
      oxygenase-1 (HO-1) in TNF-alpha-treated HUVEC. Angptl7 conducted TNF-alpha-induced 
      oxidative stress and cell adhesion in HUVEC. Therefore, Angptl7 might participate 
      in the development of endothelial injury and further atherosclerosis. This might 
      give us a new insight for investigation of procession of atherosclerosis.
FAU - Li, Jiong
AU  - Li J
AD  - Key Laboratory of Preclinical Study for New Drugs of Gansu Province, School of 
      Basic Medical Sciences, Lanzhou University, Lanzhou 730000, China.
FAU - Liang, Tianxiang
AU  - Liang T
FAU - Wang, Yanzheng
AU  - Wang Y
FAU - Gan, Yirong
AU  - Gan Y
FAU - Kou, Zongke
AU  - Kou Z
FAU - Zhang, Yunlong
AU  - Zhang Y
FAU - Mao, Rui
AU  - Mao R
FAU - Xie, Dingxiong
AU  - Xie D
LA  - eng
PT  - Journal Article
PL  - Slovakia
TA  - Gen Physiol Biophys
JT  - General physiology and biophysics
JID - 8400604
RN  - 0 (ANGPTL7 protein, human)
RN  - 0 (Angiopoietin-Like Protein 7)
RN  - 0 (Angiopoietin-like Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Angiopoietin-Like Protein 7
MH  - Angiopoietin-like Proteins/*genetics
MH  - *Cell Adhesion
MH  - Cells, Cultured
MH  - Human Umbilical Vein Endothelial Cells/*cytology
MH  - Humans
MH  - *Oxidative Stress
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 2020/06/12 06:00
MHDA- 2020/09/12 06:00
CRDT- 2020/06/12 06:00
PHST- 2019/08/05 00:00 [received]
PHST- 2019/12/15 00:00 [accepted]
PHST- 2020/06/12 06:00 [entrez]
PHST- 2020/06/12 06:00 [pubmed]
PHST- 2020/09/12 06:00 [medline]
AID - 10.4149/gpb_2019062 [doi]
PST - ppublish
SO  - Gen Physiol Biophys. 2020 May;39(3):285-292. doi: 10.4149/gpb_2019062.