PMID- 32528054
OWN - NLM
STAT- MEDLINE
DCOM- 20201130
LR  - 20231103
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 10
IP  - 1
DP  - 2020 Jun 11
TI  - Comparison of lung microbiota between antineutrophil cytoplasmic 
      antibody-associated vasculitis and sarcoidosis.
PG  - 9466
LID - 10.1038/s41598-020-66178-4 [doi]
LID - 9466
AB  - Microbial involvement in the pathogenesis have been suggested in both 
      antineutrophil cytoplasmic antibody-associated vasculitis (AAV) and sarcoidosis, 
      both of which have lung involvement. However, exhaustive research to assess the 
      bacteria in the lung in AAV and in sarcoidosis have not been performed. We sought 
      to elucidate the distinct dysbiotic lung microbiota between AAV and sarcoidosis. 
      We used 16S rRNA gene high-throughput sequencing to obtain the bacterial 
      community composition of bronchoalveolar lavage fluid (BALF) in patients with AAV 
      (n = 16) compared to patients with sarcoidosis (n = 21). The patients had not 
      undergone therapy with immunosuppressive medication when their BALF was acquired. 
      No difference was observed in alpha-diversity between patients with AAV and patients 
      with sarcoidosis when using all the detected taxa. We defined the taxa of the 
      oral cavity by using the data of oral microbiota of healthy individuals from the 
      Human Microbiome Project (HMP). The analysis using only oral taxa made the 
      difference in alpha-diversity between AAV and sarcoidosis clearer compared with those 
      using all the detected taxa. Besides, the analysis using detected taxa except for 
      oral taxa also made the difference in alpha-diversity between AAV and sarcoidosis 
      clearer compared with those using all the detected taxa. A linear negative 
      relationship between the alpha-diversity and Birmingham vasculitis activity score 
      (BVAS) was detected in the AAV group. The observed p-value for the effect of the 
      disease groups on the ss-diversity was small while the effect of other factors 
      including sex and smoking status did not have small p-values. By excluding oral 
      taxa from all the detected taxa, we found a cluster mainly consisted of 
      sarcoidosis patients which was characterized with microbial community monopolized 
      by Erythrobacteraceae family. Our results suggested the importance of considering 
      the influence of oral microbiota in evaluating lung microbiota.
FAU - Fukui, Shoichi
AU  - Fukui S
AUID- ORCID: 0000-0001-5487-4692
AD  - Department of Immunology and Rheumatology, Nagasaki University Graduate School of 
      Biomedical Sciences, Nagasaki, Japan.
AD  - Department of Community Medicine, Nagasaki University Graduate School of 
      Biomedical Sciences, Nagasaki, Japan.
FAU - Morimoto, Shimpei
AU  - Morimoto S
AUID- ORCID: 0000-0002-5340-6851
AD  - Innovation Platform & Office for Precision Medicine, Nagasaki University Graduate 
      School of Biomedical Sciences, Nagasaki, Japan.
FAU - Ichinose, Kunihiro
AU  - Ichinose K
AUID- ORCID: 0000-0001-8794-7106
AD  - Department of Immunology and Rheumatology, Nagasaki University Graduate School of 
      Biomedical Sciences, Nagasaki, Japan. kichinos@nagasaki-u.ac.jp.
FAU - Nakashima, Shota
AU  - Nakashima S
AD  - Department of Respiratory Medicine, Nagasaki University Graduate School of 
      Biomedical Sciences, Nagasaki, Japan.
FAU - Ishimoto, Hiroshi
AU  - Ishimoto H
AD  - Department of Respiratory Medicine, Nagasaki University Graduate School of 
      Biomedical Sciences, Nagasaki, Japan.
FAU - Hara, Atsuko
AU  - Hara A
AD  - Department of Respiratory Medicine, Nagasaki University Graduate School of 
      Biomedical Sciences, Nagasaki, Japan.
FAU - Kakugawa, Tomoyuki
AU  - Kakugawa T
AD  - Department of Respiratory Medicine, Nagasaki University Graduate School of 
      Biomedical Sciences, Nagasaki, Japan.
FAU - Sakamoto, Noriho
AU  - Sakamoto N
AUID- ORCID: 0000-0002-7577-476X
AD  - Department of Respiratory Medicine, Nagasaki University Graduate School of 
      Biomedical Sciences, Nagasaki, Japan.
FAU - Tsuji, Yoshika
AU  - Tsuji Y
AD  - Rheumatic and Collagen Disease Center, Sasebo Chuo Hospital, Sasebo, Japan.
FAU - Aramaki, Toshiyuki
AU  - Aramaki T
AD  - Rheumatic and Collagen Disease Center, Sasebo Chuo Hospital, Sasebo, Japan.
FAU - Koga, Tomohiro
AU  - Koga T
AD  - Department of Immunology and Rheumatology, Nagasaki University Graduate School of 
      Biomedical Sciences, Nagasaki, Japan.
AD  - Center for Bioinformatics and Molecular Medicine, Nagasaki University Graduate 
      School of Biomedical Sciences, Nagasaki, Japan.
FAU - Kawashiri, Shin-Ya
AU  - Kawashiri SY
AD  - Department of Immunology and Rheumatology, Nagasaki University Graduate School of 
      Biomedical Sciences, Nagasaki, Japan.
AD  - Department of Community Medicine, Nagasaki University Graduate School of 
      Biomedical Sciences, Nagasaki, Japan.
FAU - Iwamoto, Naoki
AU  - Iwamoto N
AD  - Department of Immunology and Rheumatology, Nagasaki University Graduate School of 
      Biomedical Sciences, Nagasaki, Japan.
FAU - Tamai, Mami
AU  - Tamai M
AUID- ORCID: 0000-0002-2429-7553
AD  - Department of Immunology and Rheumatology, Nagasaki University Graduate School of 
      Biomedical Sciences, Nagasaki, Japan.
FAU - Nakamura, Hideki
AU  - Nakamura H
AD  - Department of Immunology and Rheumatology, Nagasaki University Graduate School of 
      Biomedical Sciences, Nagasaki, Japan.
FAU - Origuchi, Tomoki
AU  - Origuchi T
AD  - Department of Immunology and Rheumatology, Nagasaki University Graduate School of 
      Biomedical Sciences, Nagasaki, Japan.
AD  - Department of Rehabilitation Sciences, Nagasaki University Graduate School of 
      Biomedical Sciences, Nagasaki, Japan.
FAU - Ueki, Yukitaka
AU  - Ueki Y
AD  - Rheumatic and Collagen Disease Center, Sasebo Chuo Hospital, Sasebo, Japan.
FAU - Suzuki, Shino
AU  - Suzuki S
AD  - Kochi Institute for Core Sample Research, X-star, Japan Agency for Marine-Earth 
      Science and Technology (JAMSTEC), Nankoku, Japan.
FAU - Mukae, Hiroshi
AU  - Mukae H
AD  - Department of Respiratory Medicine, Nagasaki University Graduate School of 
      Biomedical Sciences, Nagasaki, Japan.
FAU - Kawakami, Atsushi
AU  - Kawakami A
AD  - Department of Immunology and Rheumatology, Nagasaki University Graduate School of 
      Biomedical Sciences, Nagasaki, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200611
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Antibodies, Antineutrophil Cytoplasmic)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Anti-Neutrophil Cytoplasmic Antibody-Associated 
      Vasculitis/immunology/*microbiology
MH  - Antibodies, Antineutrophil Cytoplasmic/*immunology
MH  - Bacteria/genetics/*immunology
MH  - Bronchoalveolar Lavage Fluid/immunology/microbiology
MH  - Female
MH  - Humans
MH  - Lung/*immunology/*microbiology
MH  - Male
MH  - Microbiota/genetics/*immunology
MH  - Middle Aged
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sarcoidosis/immunology/*microbiology
PMC - PMC7289840
COIS- The authors declare no competing interests.
EDAT- 2020/06/13 06:00
MHDA- 2020/12/01 06:00
PMCR- 2020/06/11
CRDT- 2020/06/13 06:00
PHST- 2018/12/16 00:00 [received]
PHST- 2020/05/14 00:00 [accepted]
PHST- 2020/06/13 06:00 [entrez]
PHST- 2020/06/13 06:00 [pubmed]
PHST- 2020/12/01 06:00 [medline]
PHST- 2020/06/11 00:00 [pmc-release]
AID - 10.1038/s41598-020-66178-4 [pii]
AID - 66178 [pii]
AID - 10.1038/s41598-020-66178-4 [doi]
PST - epublish
SO  - Sci Rep. 2020 Jun 11;10(1):9466. doi: 10.1038/s41598-020-66178-4.