PMID- 32528503
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1664-462X (Print)
IS  - 1664-462X (Electronic)
IS  - 1664-462X (Linking)
VI  - 11
DP  - 2020
TI  - Cell Wall Acetylation in Hybrid Aspen Affects Field Performance, Foliar Phenolic 
      Composition and Resistance to Biological Stress Factors in a Construct-Dependent 
      Fashion.
PG  - 651
LID - 10.3389/fpls.2020.00651 [doi]
LID - 651
AB  - The production of biofuels and "green" chemicals from the lignocellulose of 
      fast-growing hardwood species is hampered by extensive acetylation of xylan. 
      Different strategies have been implemented to reduce xylan acetylation, resulting 
      in transgenic plants that show good growth in the greenhouse, improved 
      saccharification and fermentation, but the field performance of such plants has 
      not yet been reported. The aim of this study was to evaluate the impact of 
      reduced acetylation on field productivity and identify the best strategies for 
      decreasing acetylation. Growth and biological stress data were evaluated for 18 
      hybrid aspen lines with 10-20% reductions in the cell wall acetyl content from a 
      five year field experiment in Southern Sweden. The reduction in acetyl content 
      was achieved either by suppressing the process of acetylation in the Golgi by 
      reducing expression of REDUCED WALL ACETYLATION (RWA) genes, or by post-synthetic 
      acetyl removal by fungal acetyl xylan esterases (AXEs) from two different 
      families, CE1 and CE5, targeting them to cell walls. Transgene expression was 
      regulated by either a constitutive promoter (35S) or a wood-specific promoter 
      (WP). For the majority of transgenic lines, growth was either similar to that in 
      WT and transgenic control (WP:GUS) plants, or slightly reduced. The slight 
      reduction was observed in the AXE-expressing lines regulated by the 35S promoter, 
      not those with the WP promoter which limits expression to cells developing 
      secondary walls. Expressing AXEs regulated by the 35S promoter resulted in 
      increased foliar arthropod chewing, and altered condensed tannins and salicinoid 
      phenolic glucosides (SPGs) profiles. Greater growth inhibition was observed in 
      the case of CE5 than with CE1 AXE, and it was associated with increased foliar 
      necrosis and distinct SPG profiles, suggesting that CE5 AXE could be recognized 
      by the pathogen-associated molecular pattern system. For each of three different 
      constructs, there was a line with dwarfism and growth abnormalities, suggesting 
      random genetic/epigenetic changes. This high frequency of dwarfism (17%) is 
      suggestive of a link between acetyl metabolism and chromatin function. These data 
      represent the first evaluation of acetyl-reduced plants from the field, 
      indicating some possible pitfalls, and identifying the best strategies, when 
      developing highly productive acetyl-reduced feedstocks.
CI  - Copyright (c) 2020 Derba-Maceluch, Amini, Donev, Pawar, Michaud, Johansson, 
      Albrectsen and Mellerowicz.
FAU - Derba-Maceluch, Marta
AU  - Derba-Maceluch M
AD  - Department of Forest Genetics and Plant Physiology, Umea Plant Science Centre, 
      Swedish University of Agricultural Sciences, Umea, Sweden.
FAU - Amini, Fariba
AU  - Amini F
AD  - Department of Plant Physiology, Umea Plant Science Centre, Umea University, Umea, 
      Sweden.
AD  - Biology Department, Faculty of Science, Arak University, Arak, Iran.
FAU - Donev, Evgeniy N
AU  - Donev EN
AD  - Department of Forest Genetics and Plant Physiology, Umea Plant Science Centre, 
      Swedish University of Agricultural Sciences, Umea, Sweden.
FAU - Pawar, Prashant Mohan-Anupama
AU  - Pawar PM
AD  - Department of Forest Genetics and Plant Physiology, Umea Plant Science Centre, 
      Swedish University of Agricultural Sciences, Umea, Sweden.
FAU - Michaud, Lisa
AU  - Michaud L
AD  - Department of Plant Physiology, Umea Plant Science Centre, Umea University, Umea, 
      Sweden.
FAU - Johansson, Ulf
AU  - Johansson U
AD  - Tonnersjoheden Experimental Forest, Swedish University of Agricultural Sciences, 
      Simlangsdalen, Sweden.
FAU - Albrectsen, Benedicte R
AU  - Albrectsen BR
AD  - Department of Plant Physiology, Umea Plant Science Centre, Umea University, Umea, 
      Sweden.
FAU - Mellerowicz, Ewa J
AU  - Mellerowicz EJ
AD  - Department of Forest Genetics and Plant Physiology, Umea Plant Science Centre, 
      Swedish University of Agricultural Sciences, Umea, Sweden.
LA  - eng
PT  - Journal Article
DEP - 20200525
PL  - Switzerland
TA  - Front Plant Sci
JT  - Frontiers in plant science
JID - 101568200
PMC - PMC7265884
OTO - NOTNLM
OT  - AnAXE1
OT  - HjAXE
OT  - Populus tremula x tremuloides
OT  - biotic resistance
OT  - condensed tannins
OT  - field trial
OT  - salicinoid phenolic glucosides
OT  - transgenic trees
EDAT- 2020/06/13 06:00
MHDA- 2020/06/13 06:01
PMCR- 2020/01/01
CRDT- 2020/06/13 06:00
PHST- 2020/02/21 00:00 [received]
PHST- 2020/04/27 00:00 [accepted]
PHST- 2020/06/13 06:00 [entrez]
PHST- 2020/06/13 06:00 [pubmed]
PHST- 2020/06/13 06:01 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - 10.3389/fpls.2020.00651 [doi]
PST - epublish
SO  - Front Plant Sci. 2020 May 25;11:651. doi: 10.3389/fpls.2020.00651. eCollection 
      2020.