PMID- 32530084
OWN - NLM
STAT- MEDLINE
DCOM- 20210621
LR  - 20210621
IS  - 2059-2310 (Electronic)
IS  - 2059-2302 (Linking)
VI  - 96
IP  - 3
DP  - 2020 Sep
TI  - A fast and reliable method for detecting SNP rs67384697 (Hsa-miR-148a binding 
      site) by a single run of allele-specific real-time PCR.
PG  - 312-322
LID - 10.1111/tan.13971 [doi]
AB  - Surface expression of human leukocyte antigen (HLA)-class I molecules is critical 
      for modulating T/natural killer lymphocytes' effector functions. Among HLA 
      molecules, HLA-C, the most recently evolved form of class I antigens, is 
      subjected to both transcriptional and multiple post-transcriptional regulation 
      mechanisms affecting its cell surface expression. Among the latter a region 
      placed in the 3' untranslated region of HLA-C transcript contains the single 
      nucleotide polymorphism (SNP) rs67384697 "G-ins/del" that has been found to be 
      strictly associated with surface levels of HLA-C allomorphs because of the effect 
      on the binding site of a microRNA (Hsa-miR-148a). Higher expression of HLA-C has 
      been proved to influence HIV-1 infection via a better control of viremia and a 
      slower disease progression. More importantly, the analysis of SNP rs67384697 
      "G-ins/del" combined with the evaluation of the HLA-Bw4/-Bw6 C1/C2 supratype, as 
      well as the killer immunoglobulin-like receptor genetic asset, has proved to be 
      pivotal in defining the status of Elite Controllers in the Caucasian population. 
      Here we describe a new reliable and fast method of allele-specific real-time PCR 
      to monitor the integrity/disruption of the binding site of the microRNA 
      Hsa-miR-148a in a high-throughput format that can be easily applied to studies 
      involving large cohorts of individuals.
CI  - (c) 2020 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Malnati, Mauro S
AU  - Malnati MS
AD  - Unit of Human Virology, Division of Immunology, Transplantation and Infectious 
      Diseases IRCCS Ospedale San Raffaele, Milan, Italy.
FAU - Biswas, Priscilla
AU  - Biswas P
AD  - Unit of Human Virology, Division of Immunology, Transplantation and Infectious 
      Diseases IRCCS Ospedale San Raffaele, Milan, Italy.
FAU - Ugolotti, Elisabetta
AU  - Ugolotti E
AD  - Translational Research Department, Laboratory Medicine, Diagnostics and Services, 
      IRCCS Istituto Giannina Gaslini, Genoa, Italy.
FAU - Di Marco, Eddi
AU  - Di Marco E
AD  - Translational Research Department, Laboratory Medicine, Diagnostics and Services, 
      IRCCS Istituto Giannina Gaslini, Genoa, Italy.
FAU - Sironi, Francesca
AU  - Sironi F
AD  - Unit of Human Virology, Division of Immunology, Transplantation and Infectious 
      Diseases IRCCS Ospedale San Raffaele, Milan, Italy.
FAU - Parolini, Francesca
AU  - Parolini F
AD  - Department of Neurosciences, Biomedicine and Movement Sciences, University of 
      Verona, Verona, Italy.
FAU - Garbarino, Lucia
AU  - Garbarino L
AD  - Histocompatibility Laboratory, Galliera Hospital, Genoa, Italy.
FAU - Mazzocco, Michela
AU  - Mazzocco M
AD  - Histocompatibility Laboratory, Galliera Hospital, Genoa, Italy.
FAU - Zipeto, Donato
AU  - Zipeto D
AD  - Department of Neurosciences, Biomedicine and Movement Sciences, University of 
      Verona, Verona, Italy.
FAU - Biassoni, Roberto
AU  - Biassoni R
AUID- ORCID: 0000-0002-4365-8166
AD  - Translational Research Department, Laboratory Medicine, Diagnostics and Services, 
      IRCCS Istituto Giannina Gaslini, Genoa, Italy.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200715
PL  - England
TA  - HLA
JT  - HLA
JID - 101675570
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Alleles
MH  - Binding Sites
MH  - Humans
MH  - *MicroRNAs/genetics
MH  - *Polymorphism, Single Nucleotide
MH  - Real-Time Polymerase Chain Reaction
OTO - NOTNLM
OT  - AIDS
OT  - HLA-C expression
OT  - Hsa-miR-148a
OT  - SNP genotyping
OT  - allele-specific qPCR
OT  - autoimmune diseases
EDAT- 2020/06/13 06:00
MHDA- 2021/06/22 06:00
CRDT- 2020/06/13 06:00
PHST- 2020/03/11 00:00 [received]
PHST- 2020/05/22 00:00 [revised]
PHST- 2020/06/10 00:00 [accepted]
PHST- 2020/06/13 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2020/06/13 06:00 [entrez]
AID - 10.1111/tan.13971 [doi]
PST - ppublish
SO  - HLA. 2020 Sep;96(3):312-322. doi: 10.1111/tan.13971. Epub 2020 Jul 15.