PMID- 32531233
OWN - NLM
STAT- MEDLINE
DCOM- 20210714
LR  - 20210714
IS  - 1872-8111 (Electronic)
IS  - 0168-0102 (Linking)
VI  - 169
DP  - 2021 Aug
TI  - Genetically encoded calcium indicators to probe complex brain circuit dynamics in 
      vivo.
PG  - 2-8
LID - S0168-0102(20)30230-3 [pii]
LID - 10.1016/j.neures.2020.05.013 [doi]
AB  - Over the past two decades, genetically encoded calcium indicators (GECIs) have 
      been used extensively to report intracellular calcium (Ca(2+)) dynamics in order 
      to readout neuronal and network activity in living tissue. Single wavelength 
      GECIs, such as GCaMP, have been widely adapted due to advances in dynamic range, 
      sensitivity, and kinetics. Additionally, recent efforts in protein engineering 
      have expanded the GECI color palette to enable direct optical interrogation of 
      more complex circuit dynamics. Here, I discuss the engineering, application, and 
      future directions of the most recently developed GECIs for in vivo neuroscience 
      research.
CI  - Copyright (c) 2020 Elsevier B.V. and Japan Neuroscience Society. All rights 
      reserved.
FAU - Inoue, Masatoshi
AU  - Inoue M
AD  - Department of Bioengineering, Stanford University, Stanford, CA 94305, USA. 
      Electronic address: minoue2@stanford.edu.
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20200609
PL  - Ireland
TA  - Neurosci Res
JT  - Neuroscience research
JID - 8500749
RN  - 0 (Indicators and Reagents)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Brain/metabolism
MH  - *Calcium/metabolism
MH  - Calcium Signaling
MH  - Indicators and Reagents
MH  - *Neurons/metabolism
OTO - NOTNLM
OT  - All-optical experiments
OT  - Calcium indicator
OT  - Genetically encoded fluorescent indicators
OT  - Multiplex imaging
OT  - Neuronal activity recording
OT  - Protein engineering
OT  - R-CaMP2
OT  - XCaMP
EDAT- 2020/06/13 06:00
MHDA- 2021/07/15 06:00
CRDT- 2020/06/13 06:00
PHST- 2020/03/29 00:00 [received]
PHST- 2020/05/13 00:00 [revised]
PHST- 2020/05/27 00:00 [accepted]
PHST- 2020/06/13 06:00 [pubmed]
PHST- 2021/07/15 06:00 [medline]
PHST- 2020/06/13 06:00 [entrez]
AID - S0168-0102(20)30230-3 [pii]
AID - 10.1016/j.neures.2020.05.013 [doi]
PST - ppublish
SO  - Neurosci Res. 2021 Aug;169:2-8. doi: 10.1016/j.neures.2020.05.013. Epub 2020 Jun 
      9.