PMID- 32533627
OWN - NLM
STAT- Publisher
LR  - 20240227
IS  - 1615-9861 (Electronic)
IS  - 1615-9853 (Linking)
DP  - 2020 Jun 12
TI  - What We See, What We Do Not See, and What We Do Not Want to See in HLA Class I 
      Immunopeptidomes.
PG  - e2000112
LID - 10.1002/pmic.202000112 [doi]
AB  - The identification of peptides bound to human leukocyte antigen class I (HLA-I) 
      molecules-that is, the HLA-I immunopeptidome-is a useful tool in the hunt for 
      epitopes suitable for vaccinations and immunotherapies. These peptides are mainly 
      generated by proteasomes through peptide hydrolysis and peptide splicing. In this 
      issue, Nicastri and colleagues compared different methods for the elution of HLA 
      class I-associated peptides. It is demonstrated that the choice of HLA-associated 
      peptide enrichment and purification strategy affects peptide yields and creates a 
      bias in detected sequence repertoire. The author carried out this technical brief 
      through the analysis of canonical non-spliced peptides. However, their study left 
      out any analysis of post-translationally spliced peptides, thereby missing an 
      opportunity to shed light on the persistent debate of the frequency of these 
      unconventional peptides.
CI  - (c) 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Mishto, Michele
AU  - Mishto M
AUID- ORCID: 0000-0003-3042-2792
AD  - Centre for Inflammation Biology and Cancer Immunology (CIBCI) & Peter Gorer 
      Department of Immunobiology, King's College London, London, SE1 1UL, UK.
LA  - eng
GR  - BRC3-IIA/National Institute for Health Research (NIHR) Biomedical Research Centre 
      based at Guy's and St Thomas' NHS Foundation Trust and King's College London 
      and/or the NIHR Clinical Research Facility/
PT  - Journal Article
DEP - 20200612
PL  - Germany
TA  - Proteomics
JT  - Proteomics
JID - 101092707
SB  - IM
OTO - NOTNLM
OT  - HLA class I immunopeptidome
OT  - elution techniques
OT  - mass spectrometry
OT  - peptide splicing
OT  - proteasomes
EDAT- 2020/06/14 06:00
MHDA- 2020/06/14 06:00
CRDT- 2020/06/14 06:00
PHST- 2020/05/28 00:00 [received]
PHST- 2020/06/14 06:00 [pubmed]
PHST- 2020/06/14 06:00 [medline]
PHST- 2020/06/14 06:00 [entrez]
AID - 10.1002/pmic.202000112 [doi]
PST - aheadofprint
SO  - Proteomics. 2020 Jun 12:e2000112. doi: 10.1002/pmic.202000112.