PMID- 32533780
OWN - NLM
STAT- MEDLINE
DCOM- 20210827
LR  - 20210827
IS  - 1470-8744 (Electronic)
IS  - 0885-4513 (Linking)
VI  - 68
IP  - 3
DP  - 2021 Jun
TI  - Determination of parvovirus antigen in the vaccine using time-resolved 
      fluorescence immunoassay.
PG  - 597-602
LID - 10.1002/bab.1967 [doi]
AB  - As a highly contagious and potentially fatal disease of dogs, canine parvovirus 
      type 2 (CPV-2) usually causes severe myocarditis and gastroenteritis, while 
      vaccine injection has greatly reduced the incidence of CPV-2 diseases. However, 
      there is currently a lack of simple and effective method for quantitative 
      detection of CPV-2 in vaccine. Therefore, this study aims to prepare an accurate 
      method to determine the CPV-2 antigen (CPV-2-Ag) in vaccine. Here, a sandwich 
      time-resolved fluorescence immunoassay (TRFIA) was established and optimized. 
      Anti-CPV-2 antibodies were immobilized on 96-well plates to capture CPV-2-Ag, and 
      then bound together with the detection antibodies labeled with Europium(III) 
      (Eu(3+) ) chelates; finally, time-resolved fluorometry was employed to measure 
      the fluorescence intensity. Vaccination was performed to evaluate the 
      relationship between CPV-2-Ag concentration and antibody titer. The sensitivity 
      is 1.15 mEU/mL (LogY = 1.524 + 0.8667 x LogX, R(2)  = 0.9933), and the average 
      recovery is among 91.00% to 106.39% without cross-reactions with the other canine 
      viral antigen. The correlation between ELISA assay and this method is up to 
      0.9861. And, there is high correlation between the CPV-2-Ag concentration and 
      antibody titers (R(2)  = 0.9234). This immunoassay established has high 
      sensitivity, accuracy, and specificity, which indicate that this method could be 
      suitable for quantitative detection of CPV-2-Ag in vaccine evaluation.
CI  - (c) 2020 International Union of Biochemistry and Molecular Biology, Inc.
FAU - Chen, Cuicui
AU  - Chen C
AD  - Guangzhou Youdi Bio-Technology Co., Ltd., Guangzhou, 510663, China.
FAU - Liang, Huankun
AU  - Liang H
AD  - Guangzhou Youdi Bio-Technology Co., Ltd., Guangzhou, 510663, China.
FAU - Hu, Baoyu
AU  - Hu B
AD  - Guangzhou Youdi Bio-Technology Co., Ltd., Guangzhou, 510663, China.
FAU - Ning, Bo
AU  - Ning B
AD  - Guangzhou Youdi Bio-Technology Co., Ltd., Guangzhou, 510663, China.
FAU - Lai, Hongrui
AU  - Lai H
AD  - Guangzhou Youdi Bio-Technology Co., Ltd., Guangzhou, 510663, China.
FAU - He, Ying
AU  - He Y
AD  - Guangzhou Youdi Bio-Technology Co., Ltd., Guangzhou, 510663, China.
FAU - Guo, Guiling
AU  - Guo G
AD  - Guangzhou Youdi Bio-Technology Co., Ltd., Guangzhou, 510663, China.
FAU - Zhong, Shuhai
AU  - Zhong S
AD  - Guangzhou Youdi Bio-Technology Co., Ltd., Guangzhou, 510663, China.
FAU - Li, Laiqing
AU  - Li L
AUID- ORCID: 0000-0002-1663-4142
AD  - Guangzhou Youdi Bio-Technology Co., Ltd., Guangzhou, 510663, China.
LA  - eng
GR  - 201906010055/Pearl River S&T Nova Program of Guangzhou/
PT  - Journal Article
DEP - 20200714
PL  - United States
TA  - Biotechnol Appl Biochem
JT  - Biotechnology and applied biochemistry
JID - 8609465
RN  - 0 (Antibodies, Viral)
RN  - 0 (Antigens, Viral)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/immunology
MH  - Antigens, Viral/*analysis/immunology
MH  - Dogs
MH  - Enzyme-Linked Immunosorbent Assay
MH  - *Fluoroimmunoassay
MH  - Parvovirus, Canine/immunology/*isolation & purification
MH  - Vaccination
MH  - Viral Vaccines/*immunology
OTO - NOTNLM
OT  - canine
OT  - parvovirus antigen
OT  - time-resolved fluorescence immunoassay
OT  - vaccine
EDAT- 2020/06/14 06:00
MHDA- 2021/08/28 06:00
CRDT- 2020/06/14 06:00
PHST- 2020/04/27 00:00 [received]
PHST- 2020/06/06 00:00 [accepted]
PHST- 2020/06/14 06:00 [pubmed]
PHST- 2021/08/28 06:00 [medline]
PHST- 2020/06/14 06:00 [entrez]
AID - 10.1002/bab.1967 [doi]
PST - ppublish
SO  - Biotechnol Appl Biochem. 2021 Jun;68(3):597-602. doi: 10.1002/bab.1967. Epub 2020 
      Jul 14.