PMID- 32534383
OWN - NLM
STAT- MEDLINE
DCOM- 20210323
LR  - 20221207
IS  - 1618-0623 (Electronic)
IS  - 0944-5013 (Linking)
VI  - 238
DP  - 2020 Sep
TI  - Whole genome shotgun sequencing of POPs degrading bacterial community dwelling 
      tannery effluents and petrol contaminated soil.
PG  - 126504
LID - S0944-5013(20)30372-4 [pii]
LID - 10.1016/j.micres.2020.126504 [doi]
AB  - The present study involved identification of genes which are present in the 
      genome of native bacteria to make them effective tools for bioremediation of 
      persistent organic pollutants (POPs). During this study, forty-one POPs 
      (naphthalene, toluene and petrol) metabolizing bacteria were isolated from 
      tannery effluents and petrol contaminated soil samples by successive enrichment 
      culturing. The taxonomic diversity and gene repertoire conferring POPs 
      degradation ability to the isolated bacterial community were studied through 
      whole genome shotgun sequencing of DNA consortium. The DNA consortium contained 
      equimolar concentration of DNA extracted from each bacterial isolate using 
      organic method. To add a double layer of confirmation the established DNA 
      consortium was subjected to 16S rRNA metagenome sequencing and whole genome 
      shotgun sequencing analysis. Biodiversity analysis revealed that the consortium 
      was composed of phyla Firmicutes (80 %), Proteobacteria (12 %) and Actinobacteria 
      (5%). Genera found included Bacillus (45 %), Burkholderia (25 %), Brevibacillus 
      (9%) and Geobacillus (4%). Functional profiling of consortium helped us to 
      identify genes associated with degradation pathways of a variety of organic 
      compounds including toluene, naphthalene, caprolactam, benzoate, aminobenzoate, 
      xylene, 4-hydroxyphenyl acetic acid, biphenyl, anthracene, aminobenzoate, 
      chlorocyclohexane, chlorobenzene, n-phenylalkanoic acid, phenylpropanoid, 
      salicylate, gentisate, central meta cleavage of aromatic compounds, cinnamic 
      acid, catechol and procatechuate branch of beta-ketoadipate pathway, phenyl-acetyl 
      CoA and homogentisate catabolic pathway. The information thus generated has 
      ensured not only biodegradation potential but also revealed many possible future 
      applications of the isolated bacteria.
CI  - Copyright (c) 2020 Elsevier GmbH. All rights reserved.
FAU - Muccee, Fatima
AU  - Muccee F
AD  - Department of Biochemistry and Biotechnology, The Islamia University of 
      Bahawalpur, Bahawalpur, Pakistan.
FAU - Ejaz, Samina
AU  - Ejaz S
AD  - Department of Biochemistry and Biotechnology, The Islamia University of 
      Bahawalpur, Bahawalpur, Pakistan. Electronic address: samina.ejazsyed@iub.edu.pk.
LA  - eng
PT  - Journal Article
DEP - 20200530
PL  - Germany
TA  - Microbiol Res
JT  - Microbiological research
JID - 9437794
RN  - 0 (DNA, Bacterial)
RN  - 0 (Gasoline)
RN  - 0 (Soil Pollutants)
RN  - 0 (Waste Water)
SB  - IM
MH  - Bacteria/*classification/*metabolism
MH  - *Biodegradation, Environmental
MH  - DNA, Bacterial
MH  - Gasoline/microbiology
MH  - Metabolic Networks and Pathways
MH  - Metagenome
MH  - Persistent Organic Pollutants/*metabolism
MH  - *Petroleum Pollution
MH  - Soil Microbiology
MH  - Soil Pollutants/metabolism
MH  - Wastewater/microbiology
MH  - Whole Genome Sequencing
OTO - NOTNLM
OT  - 16S rRNA gene sequencing
OT  - Bioremediation
OT  - Degradation
OT  - Illumina next generation sequencing
OT  - Metagenomics
OT  - Persistent organic pollutants
OT  - Whole-genome shotgun sequencing
COIS- Declaration of Competing Interest The authors declare that they have no conflicts 
      of interest.
EDAT- 2020/06/14 06:00
MHDA- 2021/03/24 06:00
CRDT- 2020/06/14 06:00
PHST- 2019/12/07 00:00 [received]
PHST- 2020/04/28 00:00 [revised]
PHST- 2020/05/01 00:00 [accepted]
PHST- 2020/06/14 06:00 [pubmed]
PHST- 2021/03/24 06:00 [medline]
PHST- 2020/06/14 06:00 [entrez]
AID - S0944-5013(20)30372-4 [pii]
AID - 10.1016/j.micres.2020.126504 [doi]
PST - ppublish
SO  - Microbiol Res. 2020 Sep;238:126504. doi: 10.1016/j.micres.2020.126504. Epub 2020 
      May 30.