PMID- 32534761
OWN - NLM
STAT- MEDLINE
DCOM- 20210224
LR  - 20210224
IS  - 0737-0806 (Print)
IS  - 0737-0806 (Linking)
VI  - 90
DP  - 2020 Jul
TI  - Decellularization of Extracellular Matrix from Equine Skeletal Muscle.
PG  - 102962
LID - S0737-0806(20)30053-8 [pii]
LID - 10.1016/j.jevs.2020.102962 [doi]
AB  - Equine represents an attractive animal model for musculoskeletal tissue diseases, 
      exhibiting much similarity to the injuries that occur in humans. Cell therapy and 
      tissue bioengineering have been widely used as a therapeutic alternative by 
      regenerative medicine in musculoskeletal diseases. Thus, the aim of this study 
      was to produce an acellular biomaterial of equine skeletal muscle and to evaluate 
      its effectiveness in supporting the in vitro culture of equine induced 
      pluripotency stem cells (iPSCs). Biceps femoris samples were frozen at -20 degrees C for 
      4 days and incubated in 1% sodium dodecyl sulfate (SDS), 5 mM EDTA + 50 mM Tris 
      and 1% Triton X-100; the effectiveness of the decellularization was evaluated by 
      the absence of remnant nuclei (histological and 4',6-diamidino-2-phenylindole 
      [DAPI] analysis), preservation of extracellular matrix (ECM) proteins 
      (immunofluorescence and immunohistochemistry) and organization of ECM 
      ultrastructure (scanning electron microscopy). Decellularized samples were 
      recellularized with iPSCs at the concentration of 50,000 cells/cm(2) and cultured 
      in vitro for 9 days, and the presence of the cells in the biomaterial was 
      evaluated by histological analysis and presence of nuclei. Decellularized 
      biomaterial showed absence of remnant nuclei and muscle fibers, as well as the 
      preservation of ECM architecture, vascular network and proteins, laminin, 
      fibronectin, elastin, collagen III and IV. After cellularization, iPSC nuclei 
      were present at 9 days after incubation, indicating the decellularized 
      biomaterial-supported iPSC survival. It is concluded that the ECM biomaterial 
      produced from the decellularized equine skeletal muscle has potential for iPSC 
      adhesion, representing a promising biomaterial for regenerative medicine in the 
      therapy of musculoskeletal diseases.
CI  - Copyright (c) 2020 Elsevier Inc. All rights reserved.
FAU - Miranda, Carla Maria Figueiredo de Carvalho
AU  - Miranda CMFC
AD  - Centre de recherche en reproduction et fertilite, Faculte de medecine 
      veterinaire, Universite de Montreal, Saint-Hyacinthe, Quebec, Canada; Department 
      of Surgery, School of Veterinary Medicine and Animal Science, University of Sao 
      Paulo, Sao Paulo, SP, Brazil. Electronic address: carlacarvalhovet@gmail.com.
FAU - Therrien, Jacinthe
AU  - Therrien J
AD  - Centre de recherche en reproduction et fertilite, Faculte de medecine 
      veterinaire, Universite de Montreal, Saint-Hyacinthe, Quebec, Canada.
FAU - Leonel, Luciano Cesar Pereira Campos
AU  - Leonel LCPC
AD  - Department of Surgery, School of Veterinary Medicine and Animal Science, 
      University of Sao Paulo, Sao Paulo, SP, Brazil.
FAU - Smith, Olivia Eilers
AU  - Smith OE
AD  - Centre de recherche en reproduction et fertilite, Faculte de medecine 
      veterinaire, Universite de Montreal, Saint-Hyacinthe, Quebec, Canada.
FAU - Miglino, Maria Angelica
AU  - Miglino MA
AD  - Department of Surgery, School of Veterinary Medicine and Animal Science, 
      University of Sao Paulo, Sao Paulo, SP, Brazil.
FAU - Smith, Lawrence Charles
AU  - Smith LC
AD  - Centre de recherche en reproduction et fertilite, Faculte de medecine 
      veterinaire, Universite de Montreal, Saint-Hyacinthe, Quebec, Canada.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200319
PL  - United States
TA  - J Equine Vet Sci
JT  - Journal of equine veterinary science
JID - 8216840
RN  - 0 (Biocompatible Materials)
RN  - 9002-93-1 (Octoxynol)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - *Biocompatible Materials
MH  - Collagen
MH  - *Extracellular Matrix
MH  - Horses
MH  - Muscle, Skeletal
MH  - Octoxynol
OTO - NOTNLM
OT  - Biomaterial
OT  - Decellularized extracellular matrix
OT  - Regenerative medicine
OT  - Skeletal muscle
OT  - iPSC
EDAT- 2020/06/15 06:00
MHDA- 2021/02/25 06:00
CRDT- 2020/06/15 06:00
PHST- 2019/03/14 00:00 [received]
PHST- 2019/07/17 00:00 [revised]
PHST- 2020/02/06 00:00 [accepted]
PHST- 2020/06/15 06:00 [entrez]
PHST- 2020/06/15 06:00 [pubmed]
PHST- 2021/02/25 06:00 [medline]
AID - S0737-0806(20)30053-8 [pii]
AID - 10.1016/j.jevs.2020.102962 [doi]
PST - ppublish
SO  - J Equine Vet Sci. 2020 Jul;90:102962. doi: 10.1016/j.jevs.2020.102962. Epub 2020 
      Mar 19.