PMID- 32536930
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1664-462X (Print)
IS  - 1664-462X (Electronic)
IS  - 1664-462X (Linking)
VI  - 11
DP  - 2020
TI  - Integrated Karyotypes of Diploid and Tetraploid Carrizo Citrange (Citrus sinensis 
      L. Osbeck x Poncirus trifoliata L. Raf.) as Determined by Sequential Multicolor 
      Fluorescence in situ Hybridization With Tandemly Repeated DNA Sequences.
PG  - 569
LID - 10.3389/fpls.2020.00569 [doi]
LID - 569
AB  - Carrizo citrange [Citrus sinensis (L.) Osbeck x Poncirus trifoliata (L.) Raf., 
      CC] is one of the most widely used rootstocks in citriculture worldwide, but its 
      cytogenetic study has been hampered by its inherent small size, morphological 
      similarity to mitotic chromosomes, and lack of accessible cytological landmarks. 
      In our previous study, a spontaneously occurring tetraploid CC seedling was 
      discovered. The main goals of this study were to elucidate the chromosome 
      constitution and construct the karyotypes of diploid CC rootstock and its 
      corresponding spontaneously occurring tetraploid. To accomplish these, the 
      chromosomal characteristics were investigated by sequential multicolor 
      fluorescence in situ hybridization (FISH) with eight properly labeled repetitive 
      DNA sequences, including a centromere-like repeat, four satellite repeats, two 
      rDNAs, and an oligonucleotide of telomeric (TTTAGGG) (n) repeat. The results 
      nicely demonstrated that these repetitive DNAs are reliable cytogenetic markers 
      that collectively facilitate simultaneous and unequivocal identification of 
      homologous chromosome pairs. Based on chromosome size and morphology together 
      with FISH patterns of repetitive DNAs, an integrated karyotype of CC rootstock 
      was constructed, consisting of 2n = 2x = 12m (1sat) + 6sm with karyotype 
      asymmetry degree being divided into 2B category. Cytogenetically speaking, the 
      variable and asymmetric distribution patterns of these repetitive DNAs were fully 
      confirmed the hybrid nature of CC rootstock. In addition, comparative 
      distribution patterns and chromosomal localizations of these repetitive DNAs 
      convincingly showed that this tetraploid CC material arose from somatic 
      chromosome doubling of diploid CC rootstock. This study revealed, for the first 
      time, the integrated karyotype and chromosomal characteristics of this important 
      citrus rootstock as well as its spontaneously occurring tetraploid plant. 
      Furthermore, this study is a good prospective model for study species with 
      morphologically indistinguishable small chromosomes.
CI  - Copyright (c) 2020 Deng, Tang, Xu, Gao, Lin, Liang, Xia, Deng, Wang, Cai, Liang and 
      Lv.
FAU - Deng, Honghong
AU  - Deng H
AD  - Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu, 
      China.
AD  - College of Horticulture and Landscape Architecture, Southwest University, 
      Chongqing, China.
FAU - Tang, Guohao
AU  - Tang G
AD  - Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu, 
      China.
FAU - Xu, Nuo
AU  - Xu N
AD  - Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu, 
      China.
FAU - Gao, Zhijian
AU  - Gao Z
AD  - Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu, 
      China.
FAU - Lin, Lijin
AU  - Lin L
AD  - Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu, 
      China.
FAU - Liang, Dong
AU  - Liang D
AD  - Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu, 
      China.
FAU - Xia, Hui
AU  - Xia H
AD  - Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu, 
      China.
FAU - Deng, Qunxian
AU  - Deng Q
AD  - Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu, 
      China.
FAU - Wang, Jin
AU  - Wang J
AD  - Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu, 
      China.
FAU - Cai, Zexi
AU  - Cai Z
AD  - National Maize Improvement Center, College of Agronomy and Biotechnology, China 
      Agricultural University, Beijing, China.
FAU - Liang, Guolu
AU  - Liang G
AD  - College of Horticulture and Landscape Architecture, Southwest University, 
      Chongqing, China.
FAU - Lv, Xiulan
AU  - Lv X
AD  - Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20200527
PL  - Switzerland
TA  - Front Plant Sci
JT  - Frontiers in plant science
JID - 101568200
PMC - PMC7267054
OTO - NOTNLM
OT  - chromosome discrimination
OT  - citrus rootstock
OT  - cytogenetic markers
OT  - oligonucleotide probe
OT  - ribosomal DNA
OT  - satellite repeat
EDAT- 2020/06/17 06:00
MHDA- 2020/06/17 06:01
PMCR- 2020/01/01
CRDT- 2020/06/16 06:00
PHST- 2019/11/29 00:00 [received]
PHST- 2020/04/16 00:00 [accepted]
PHST- 2020/06/16 06:00 [entrez]
PHST- 2020/06/17 06:00 [pubmed]
PHST- 2020/06/17 06:01 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - 10.3389/fpls.2020.00569 [doi]
PST - epublish
SO  - Front Plant Sci. 2020 May 27;11:569. doi: 10.3389/fpls.2020.00569. eCollection 
      2020.