PMID- 32537798
OWN - NLM
STAT- MEDLINE
DCOM- 20210329
LR  - 20210329
IS  - 1939-165X (Electronic)
IS  - 0275-6382 (Linking)
VI  - 49
IP  - 2
DP  - 2020 Jun
TI  - Serum hepcidin measurements in healthy dogs using liquid chromatography/tandem 
      mass spectrometry.
PG  - 292-298
LID - 10.1111/vcp.12872 [doi]
AB  - BACKGROUND: Hepcidin is a key regulator of iron homeostasis. The measurement of 
      this hormone is essential for the diagnosis of iron deficiency anemia and might 
      be useful as a prognostic factor in many diseases. Serum hepcidin levels have 
      been infrequently evaluated in dogs. OBJECTIVES: The aim of this study was to 
      measure serum hepcidin in a population of healthy dogs using liquid 
      chromatography/tandem mass spectrometry (LC-MS/MS), considered the gold standard 
      assay for measuring hepcidin like in human medicine. METHODS: Blood samples from 
      86 healthy dogs of 25 different breeds were measured with the LC-MS/MS method. 
      Synthetic canine hepcidin was used as the standard reagent. Reference values were 
      calculated based on the results. RESULTS: The mean hepcidin concentration of the 
      study population was 16.6 +/- 7.7 ng/mL. There reference interval (RI) was defined 
      as 5.3-36.4 ng/mL. No significant difference was found between male and female 
      dogs, or between different age and body weight groups. Hepcidin concentrations 
      did not correlate with red blood cell counts, hemoglobin concentrations, iron 
      levels, iron-binding capacities, and C-reactive protein concentrations in this 
      healthy population. A weak negative correlation was found between hepcidin and 
      the mean corpuscular volume. CONCLUSION: LC-MS/MS proved to be a reliable and 
      time-effective method for the detection of canine hepcidin. The RI was similar 
      but narrower compared with that of human studies.
CI  - (c) 2020 The Authors. Veterinary Clinical Pathology published by Wiley Periodicals 
      LLC on behalf of American Society for Veterinary Clinical Pathology.
FAU - Vizi, Zsuzsanna
AU  - Vizi Z
AUID- ORCID: 0000-0001-9109-7482
AD  - Department of Internal Medicine, University of Veterinary Medicine, Budapest, 
      Hungary.
FAU - Lanyi, Katalin
AU  - Lanyi K
AD  - Department of Food Hygiene, University of Veterinary Medicine, Budapest, Hungary.
FAU - Bagi, Melinda
AU  - Bagi M
AD  - Department of Internal Medicine, University of Veterinary Medicine, Budapest, 
      Hungary.
FAU - Laczay, Peter
AU  - Laczay P
AD  - Department of Food Hygiene, University of Veterinary Medicine, Budapest, Hungary.
FAU - Balogh, Nandor
AU  - Balogh N
AD  - Praxislab Kft, Budapest, Hungary.
FAU - Sterczer, Agnes
AU  - Sterczer A
AD  - Department of Internal Medicine, University of Veterinary Medicine, Budapest, 
      Hungary.
LA  - eng
GR  - 69P00RH03/New National Excellence Program of the Ministry of Human Capacities, 
      Hungary/
PT  - Journal Article
DEP - 20200614
PL  - United States
TA  - Vet Clin Pathol
JT  - Veterinary clinical pathology
JID - 9880575
RN  - 0 (Hepcidins)
SB  - IM
MH  - Animals
MH  - Chromatography, Liquid/veterinary
MH  - Dogs/*blood
MH  - Female
MH  - Hepcidins/*blood
MH  - Male
MH  - Reference Values
MH  - Tandem Mass Spectrometry/veterinary
OTO - NOTNLM
OT  - LC-MS/MS
OT  - canine
OT  - hepcidin
OT  - iron
OT  - reference interval
EDAT- 2020/06/17 06:00
MHDA- 2021/03/30 06:00
CRDT- 2020/06/16 06:00
PHST- 2019/06/04 00:00 [received]
PHST- 2019/10/15 00:00 [revised]
PHST- 2019/10/22 00:00 [accepted]
PHST- 2020/06/17 06:00 [pubmed]
PHST- 2021/03/30 06:00 [medline]
PHST- 2020/06/16 06:00 [entrez]
AID - 10.1111/vcp.12872 [doi]
PST - ppublish
SO  - Vet Clin Pathol. 2020 Jun;49(2):292-298. doi: 10.1111/vcp.12872. Epub 2020 Jun 
      14.