PMID- 32538201
OWN - NLM
STAT- MEDLINE
DCOM- 20210629
LR  - 20210629
IS  - 1502-7708 (Electronic)
IS  - 0036-5521 (Linking)
VI  - 55
IP  - 6
DP  - 2020 Jun
TI  - Heat shock transcription factor 2 predicts mucosal healing and promotes mucosal 
      repair of ulcerative colitis.
PG  - 677-686
LID - 10.1080/00365521.2020.1774924 [doi]
AB  - Background: Mucosal healing(MH) is a treatment goal in ulcerative colitis (UC). 
      Our previous studies showed heat shock transcription factor 2 (HSF2) was 
      positively correlated with the activity of UC and had anti-inflammatory potential 
      in DSS-induced colitis, but the role of HSF2 in MH remains unknown. This study 
      aimed to reveal the predictive value and mechanisms of HSF2 in the MH of 
      UC.Methods: Fecal samples were collected from 51 UC patients and 10 healthy 
      controls. Correlation analyses among HSF2, fecal calprotectin(FC) and Mayo 
      endoscopic subscore(MES) were conducted by Pearson correlation coefficient. 
      Diagnostic accuracy and cutoffs to predict MH were analyzed by ROC curves. 231 UC 
      patients were enrolled to verify the diagnostic validity of the cutoffs. HSF2 
      siRNA and HSF2-FLAG recombinant plasmids were transfected into HT-29 cells. 
      IL-1beta, TNF-alpha and TGF-beta levels in supernatants were determined by ELISA. The 
      expression and phosphorylation levels of MAPKs and Smad2/3 were detected by 
      Western blotting.Results: Positive correlations existed between HSF2 and MES 
      (r = 0.81), FC and MES (r = 0.85), and HSF2 and FC (r = 0.91). Optimal cutoffs of 
      HSF2 was 1.97 ng/ml (AUC 0.919) and that of FC was 678 microg/g (AUC 0.958). HSF2 and 
      FC achieved high sensitivity (73.7% vs 84.2%) and negative predictive value 
      (89.1% vs 93.9%). HSF2 decreased IL-1beta and TNF-alpha secretion via suppression of 
      MAPK signaling pathway activation. HSF2 promoted the expression of TGF-beta via 
      increasing phosphorylation of Smad2/3.Conclusions: HSF2 may be a predictor of MH 
      in UC patients. HSF2 inhibited inflammation and promoted mucosal repair.
FAU - Wen, Yunling
AU  - Wen Y
AUID- ORCID: 0000-0002-8197-0599
AD  - Department of Gastroenterology, The First Affiliated Hospital of Kunming Medical 
      University, Yunnan Institute of Digestive Diseases, Kunming, China.
FAU - Niu, Junkun
AU  - Niu J
AD  - Department of Gastroenterology, The First Affiliated Hospital of Kunming Medical 
      University, Yunnan Institute of Digestive Diseases, Kunming, China.
FAU - Zhang, Fengrui
AU  - Zhang F
AD  - Department of Gastroenterology, The First Affiliated Hospital of Kunming Medical 
      University, Yunnan Institute of Digestive Diseases, Kunming, China.
FAU - Wu, Jing
AU  - Wu J
AD  - Department of Gastroenterology, The First Affiliated Hospital of Kunming Medical 
      University, Yunnan Institute of Digestive Diseases, Kunming, China.
FAU - Li, Maojuan
AU  - Li M
AD  - Department of Gastroenterology, The First Affiliated Hospital of Kunming Medical 
      University, Yunnan Institute of Digestive Diseases, Kunming, China.
FAU - Sun, Yang
AU  - Sun Y
AD  - Department of Gastroenterology, The First Affiliated Hospital of Kunming Medical 
      University, Yunnan Institute of Digestive Diseases, Kunming, China.
FAU - Wang, Wen
AU  - Wang W
AD  - Department of Gastroenterology, The First Affiliated Hospital of Kunming Medical 
      University, Yunnan Institute of Digestive Diseases, Kunming, China.
FAU - Xia, Shuxian
AU  - Xia S
AD  - Department of Gastroenterology, The First Affiliated Hospital of Kunming Medical 
      University, Yunnan Institute of Digestive Diseases, Kunming, China.
FAU - Tan, Ying
AU  - Tan Y
AD  - Department of Gastroenterology, The First Affiliated Hospital of Kunming Medical 
      University, Yunnan Institute of Digestive Diseases, Kunming, China.
FAU - Wang, Kunhua
AU  - Wang K
AD  - Department of General Surgery, The First Affiliated Hospital of Kunming Medical 
      University, Yunnan Institute of Digestive Diseases, Kunming, China.
FAU - Miao, Yinglei
AU  - Miao Y
AD  - Department of Gastroenterology, The First Affiliated Hospital of Kunming Medical 
      University, Yunnan Institute of Digestive Diseases, Kunming, China.
LA  - eng
PT  - Journal Article
DEP - 20200613
PL  - England
TA  - Scand J Gastroenterol
JT  - Scandinavian journal of gastroenterology
JID - 0060105
RN  - 0 (Biomarkers)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Leukocyte L1 Antigen Complex)
RN  - 0 (Transcription Factors)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 142297-91-4 (HSF2 protein, human)
SB  - IM
MH  - Adult
MH  - Biomarkers/analysis/metabolism
MH  - Case-Control Studies
MH  - Colitis, Ulcerative/*metabolism/pathology
MH  - Colonoscopy
MH  - Feces/*chemistry
MH  - Female
MH  - HT29 Cells
MH  - Heat-Shock Proteins/genetics/*metabolism
MH  - Humans
MH  - Interleukin-1beta/metabolism
MH  - Intestinal Mucosa/*metabolism/pathology
MH  - Leukocyte L1 Antigen Complex/*analysis
MH  - Male
MH  - Middle Aged
MH  - ROC Curve
MH  - Severity of Illness Index
MH  - Transcription Factors/genetics/*metabolism
MH  - Tumor Necrosis Factor-alpha/metabolism
OTO - NOTNLM
OT  - Ulcerative colitis
OT  - heat shock transcription factor 2
OT  - mechanisms
OT  - mucosal healing
OT  - predictive value
EDAT- 2020/06/17 06:00
MHDA- 2021/06/30 06:00
CRDT- 2020/06/16 06:00
PHST- 2020/06/17 06:00 [pubmed]
PHST- 2021/06/30 06:00 [medline]
PHST- 2020/06/16 06:00 [entrez]
AID - 10.1080/00365521.2020.1774924 [doi]
PST - ppublish
SO  - Scand J Gastroenterol. 2020 Jun;55(6):677-686. doi: 
      10.1080/00365521.2020.1774924. Epub 2020 Jun 13.