PMID- 32538668
OWN - NLM
STAT- MEDLINE
DCOM- 20201215
LR  - 20211204
IS  - 0028-2685 (Print)
IS  - 0028-2685 (Linking)
VI  - 67
IP  - 5
DP  - 2020 Sep
TI  - The knockdown of SNHG3 inhibits the progression of laryngeal squamous cell 
      carcinoma by miR-340-5p/YAP1 axis and Wnt/beta-catenin pathway.
PG  - 1094-1105
LID - 191022N1073 [pii]
LID - 10.4149/neo_2020_191022N1073 [doi]
AB  - Laryngeal squamous cell carcinoma (LSCC) is a common malignancy of the head and 
      neck. Long noncoding RNAs (lncRNAs) play essential roles in the development and 
      treatment of LSCC. However, the role and regulatory mechanism of lncRNA small 
      nucleolar RNA host gene 3 (SNHG3) in LSCC progression remain largely unknown. 
      Twenty-five paired LSCC tissues and normal samples were collected. The expression 
      levels of SNHG3, Yes-associated protein 1 (YAP1), and microRNA-340-5p 
      (miR-340-5p) were measured via quantitative real-time polymerase chain reaction 
      or western blot. Cell viability, apoptosis, and glycolysis were investigated by 
      3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide, flow cytometry, 
      and specific assay kit, respectively. The association between SNHG3 and 
      miR-340-5p or miR-340-5p and YAP1 was assessed by dual-luciferase reporter assay. 
      The expression of a protein involved in the Wnt/beta-catenin pathway was detected by 
      western blot. The xenograft model was established to assess the anti-cancer role 
      of SNHG3 inhibition in vivo. We found that the levels of SNHG3 and YAP1 were 
      increased but the miR-340-5p expression was decreased in LSCC tissues and cells. 
      The knockdown of SNHG3 or YAP1 inhibited cell viability and glycolysis but 
      induced apoptosis in LSCC cells. Overexpression of YAP1 reversed the effect of 
      SNHG3 knockdown on LSCC progression. SNHG3 could regulate YAP1 expression by 
      competitively binding with miR-340-5p. Overexpression of miR-340-5p suppressed 
      cell viability and glycolysis but promoted apoptosis in LSCC cells. Knockdown of 
      SNHG3 repressed Wnt/beta-catenin pathway by regulating miR-340-5p and YAP1. The 
      silencing of SNHG3 reduced LSCC xenograft tumor growth. In conclusion, knockdown 
      of SNHG3 inhibited LSCC progression via inactivating Wnt/beta-catenin pathway by 
      regulating the miR-340-5p/YAP1 axis.
FAU - Kang, R
AU  - Kang R
AD  - Department of Otolaryngology, The Zhoukou Central Hospital, Zhoukou, China.
FAU - Yao, D F
AU  - Yao DF
AD  - Department of Otolaryngology and Head and Neck Surgery, The First Affiliated 
      Hospital of Guangxi Medical University, Nanning, China.
FAU - Xu, G Z
AU  - Xu GZ
AD  - Department of Otolaryngology, Shang Cai Xie He Hospital, Zhumadian, China.
FAU - Zhou, Y H
AU  - Zhou YH
AD  - Department of Otolaryngology, The First Affiliated Hospital of Henan University 
      of Science and Technology, Luoyang, China.
LA  - eng
PT  - Journal Article
DEP - 20200616
PL  - Slovakia
TA  - Neoplasma
JT  - Neoplasma
JID - 0377266
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (MIRN340 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (SNHG32 lncRNA, human)
RN  - 0 (Transcription Factors)
RN  - 0 (YAP-Signaling Proteins)
RN  - 0 (YAP1 protein, human)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/*genetics
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Gene Expression Regulation, Neoplastic
MH  - Gene Knockdown Techniques
MH  - Humans
MH  - Laryngeal Neoplasms/*genetics/pathology
MH  - MicroRNAs/*genetics
MH  - Neoplasm Proteins/*genetics
MH  - RNA, Long Noncoding
MH  - Squamous Cell Carcinoma of Head and Neck/*genetics/pathology
MH  - Transcription Factors/*genetics
MH  - *Wnt Signaling Pathway
MH  - YAP-Signaling Proteins
EDAT- 2020/06/17 06:00
MHDA- 2020/12/16 06:00
CRDT- 2020/06/16 06:00
PHST- 2019/10/22 00:00 [received]
PHST- 2020/01/22 00:00 [accepted]
PHST- 2020/06/17 06:00 [pubmed]
PHST- 2020/12/16 06:00 [medline]
PHST- 2020/06/16 06:00 [entrez]
AID - 191022N1073 [pii]
AID - 10.4149/neo_2020_191022N1073 [doi]
PST - ppublish
SO  - Neoplasma. 2020 Sep;67(5):1094-1105. doi: 10.4149/neo_2020_191022N1073. Epub 2020 
      Jun 16.