PMID- 32540489
OWN - NLM
STAT- MEDLINE
DCOM- 20210203
LR  - 20210203
IS  - 1095-6840 (Electronic)
IS  - 0016-6480 (Linking)
VI  - 296
DP  - 2020 Sep 15
TI  - Gemfibrozil improves lipid metabolism in Nile tilapia Oreochromis niloticus fed a 
      high-carbohydrate diet through peroxisome proliferator activated receptor-alpha 
      activation.
PG  - 113537
LID - S0016-6480(20)30290-2 [pii]
LID - 10.1016/j.ygcen.2020.113537 [doi]
AB  - High carbohydrate diet (HCD) can induce lipid metabolism disorder, characterized 
      by excessive lipid in farmed fish. Peroxisome proliferator activated receptor-alpha 
      (PPARalpha) plays an important role in lipid homeostasis. In this study, we 
      hypothesize that PPARalpha can improve lipid metabolism in fish fed HCD. Fish 
      (3.03 +/- 0.11 g) were fed with three diets: control (30% carbohydrate), HCD (45% 
      carbohydrate) and HCG (HCD supplemented with 200 mg/kg gemfibrozil, an agonist of 
      PPARalpha) for eight weeks. The fish fed HCG had higher growth rate and protein 
      effiency than those fed the HCD diet, whereas the opposite trend was observed in 
      feed conversion ratio, hepatosomatic index and mesenteric fat index. 
      Additionally, fish fed HCG significantly decreased lipid accumulation in the 
      whole body, liver and adipose tissues compared to those fed the HCD diet. 
      Furthermore, fish in the HCG group significantly increased the mRNA and protein 
      expression and protein dephosphorylation of PPARalpha. The HCG group also 
      significantly increased the mRNA level of the downstream target genes of PPARalpha, 
      whereas the opposite trend occured in the mRNA level of lipolysis-related genes 
      compared to the HCD group. Besides, fish in the HCG group remarkably decreased 
      the contents of alanine aminotransferase, aspartate aminotransferase and 
      malondialdehyde, whereas the opposite occured in the activities of antioxidative 
      enzymes and anti-inflammatory cytokine genes compared to the HCD group. This 
      study indicates that gemfibrozil can improve lipid metabolism and maintain high 
      antioxidant and anti-inflammatory capacity through activating PPARalpha in Nile 
      tilapia fed a high carbohydrate diet.
CI  - Copyright (c) 2020 Elsevier Inc. All rights reserved.
FAU - Luo, Yuan
AU  - Luo Y
AD  - Laboratory of Aquaculture Nutrition and Environmental Health (LANEH), School of 
      Life Sciences, East China Normal University, 500 Dongchuan Rd, Shanghai 200241, 
      China.
FAU - Hu, Chun-Ting
AU  - Hu CT
AD  - Laboratory of Aquaculture Nutrition and Environmental Health (LANEH), School of 
      Life Sciences, East China Normal University, 500 Dongchuan Rd, Shanghai 200241, 
      China.
FAU - Qiao, Fang
AU  - Qiao F
AD  - Laboratory of Aquaculture Nutrition and Environmental Health (LANEH), School of 
      Life Sciences, East China Normal University, 500 Dongchuan Rd, Shanghai 200241, 
      China.
FAU - Wang, Xiao-Dan
AU  - Wang XD
AD  - Laboratory of Aquaculture Nutrition and Environmental Health (LANEH), School of 
      Life Sciences, East China Normal University, 500 Dongchuan Rd, Shanghai 200241, 
      China.
FAU - Qin, Jian G
AU  - Qin JG
AD  - College of Science and Engineering, Flinders University, Adelaide, SA 5001, 
      Australia.
FAU - Du, Zhen-Yu
AU  - Du ZY
AD  - Laboratory of Aquaculture Nutrition and Environmental Health (LANEH), School of 
      Life Sciences, East China Normal University, 500 Dongchuan Rd, Shanghai 200241, 
      China. Electronic address: zydu@bio.ecnu.edu.cn.
FAU - Chen, Li-Qiao
AU  - Chen LQ
AD  - Laboratory of Aquaculture Nutrition and Environmental Health (LANEH), School of 
      Life Sciences, East China Normal University, 500 Dongchuan Rd, Shanghai 200241, 
      China. Electronic address: lqchen@bio.ecnu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200612
PL  - United States
TA  - Gen Comp Endocrinol
JT  - General and comparative endocrinology
JID - 0370735
RN  - 0 (Antioxidants)
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (PPAR alpha)
RN  - 0 (RNA, Messenger)
RN  - Q8X02027X3 (Gemfibrozil)
SB  - IM
MH  - Adipose Tissue/drug effects/metabolism
MH  - Animals
MH  - Antioxidants/metabolism
MH  - Body Composition/drug effects
MH  - Cichlids/blood/genetics/growth & development/*metabolism
MH  - Diet
MH  - Dietary Carbohydrates/*pharmacology
MH  - *Feeding Behavior
MH  - Gemfibrozil/*pharmacology
MH  - Inflammation/genetics/pathology
MH  - Lipid Metabolism/*drug effects
MH  - Liver/drug effects/metabolism
MH  - PPAR alpha/*metabolism
MH  - Phosphorylation/drug effects
MH  - RNA, Messenger/genetics/metabolism
MH  - Stress, Physiological/genetics
OTO - NOTNLM
OT  - Gemfibrozil
OT  - High carbohydrate diet
OT  - Lipid accumulation
OT  - Oreochromis niloticus
OT  - PPARalpha activation
EDAT- 2020/06/17 06:00
MHDA- 2021/02/04 06:00
CRDT- 2020/06/17 06:00
PHST- 2019/11/09 00:00 [received]
PHST- 2020/05/27 00:00 [revised]
PHST- 2020/06/08 00:00 [accepted]
PHST- 2020/06/17 06:00 [pubmed]
PHST- 2021/02/04 06:00 [medline]
PHST- 2020/06/17 06:00 [entrez]
AID - S0016-6480(20)30290-2 [pii]
AID - 10.1016/j.ygcen.2020.113537 [doi]
PST - ppublish
SO  - Gen Comp Endocrinol. 2020 Sep 15;296:113537. doi: 10.1016/j.ygcen.2020.113537. 
      Epub 2020 Jun 12.