PMID- 32541828
OWN - NLM
STAT- MEDLINE
DCOM- 20201207
LR  - 20231111
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 10
IP  - 1
DP  - 2020 Jun 15
TI  - Physiological response of mammary glands to Escherichia coli infection:A conflict 
      between glucose need for milk production and immune response.
PG  - 9602
LID - 10.1038/s41598-020-66612-7 [doi]
LID - 9602
AB  - The mammary immune and physiological responses to distinct mammary-pathogenic E. 
      coli (MPEC) strains were studied. One gland in each of ten cows were challenged 
      intra-mammary and milk composition (lactose, fat, total protein, casein), 
      biochemical (glucose, glucose-6-phosphate (Glu6P), oxalate, malate, lactate, 
      pyruvate and citrate, malate and lactate dehydrogenases, lactate dehydrogenase 
      (LDH), nitrite, lactic peroxidase, catalase, albumin, lactoferrin, 
      immunoglobulin) and clotting parameters were followed for 35 days post-challenge. 
      Challenge lead to clinical acute mastitis, with peak bacterial counts in milk at 
      16-24 h post-challenge. Biochemical and clotting parameters in milk reported were 
      partially in accord with lipopolysaccharide-induced mastitis, but increased Glu6P 
      and LDH activity and prolonged lactate dehydrogenase and Glu6P/Glu alterations 
      were found. Some alterations measured in milk resolved within days after 
      challenge, while others endured for above one month, regardless of bacterial 
      clearance, and some reflected physiological responses to mastitis such as the 
      balance between aerobic and anaerobic metabolism (citrate to lactate ratios). The 
      results suggest that E. coli mastitis can be divided into two stages: an acute, 
      clinical phase, as an immediate response to bacterial infection in the mammary 
      gland, and a chronic phase, independent of bacteria clearance, in response to 
      tissue damage caused during the acute phase.
FAU - Blum, Shlomo E
AU  - Blum SE
AD  - National Mastitis Reference Center, Kimron Veterinary Institute, Ministry of 
      Agriculture and Rural Development, P.O. Box 6, Bet Dagan, 50250, Israel.
AD  - Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and 
      Environment, The Hebrew University of Jerusalem, P.O.Box 12, Rehovot, 76100, 
      Israel.
FAU - Heller, Dan E
AU  - Heller DE
AD  - Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and 
      Environment, The Hebrew University of Jerusalem, P.O.Box 12, Rehovot, 76100, 
      Israel.
FAU - Jacoby, Shamay
AU  - Jacoby S
AD  - Institute of Animal Science, A.R.O. The Volcani Center, P.O. Box 6, Bet Dagan, 
      50250, Israel.
FAU - Krifuks, Oleg
AU  - Krifuks O
AD  - National Mastitis Reference Center, Kimron Veterinary Institute, Ministry of 
      Agriculture and Rural Development, P.O. Box 6, Bet Dagan, 50250, Israel.
FAU - Merin, Uzi
AU  - Merin U
AD  - Food Quality and Safety, Postharvest and Food Sciences, A.R.O. The Volcani 
      Center, P.O. Box 6, Bet Dagan, 50250, Israel.
FAU - Silanikove, Nissim
AU  - Silanikove N
AD  - Institute of Animal Science, A.R.O. The Volcani Center, P.O. Box 6, Bet Dagan, 
      50250, Israel.
FAU - Lavon, Yaniv
AU  - Lavon Y
AD  - Israel Cattle Breeders Association, Caesarea, 38900, Israel.
FAU - Edery, Nir
AU  - Edery N
AD  - National Mastitis Reference Center, Kimron Veterinary Institute, Ministry of 
      Agriculture and Rural Development, P.O. Box 6, Bet Dagan, 50250, Israel.
FAU - Leitner, Gabriel
AU  - Leitner G
AD  - National Mastitis Reference Center, Kimron Veterinary Institute, Ministry of 
      Agriculture and Rural Development, P.O. Box 6, Bet Dagan, 50250, Israel. 
      leitnerg1@gmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200615
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Milk Proteins)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
EIN - Sci Rep. 2021 Mar 2;11(1):5398. PMID: 33654127
MH  - Animals
MH  - Cattle
MH  - Escherichia coli Infections/immunology/physiopathology/*veterinary
MH  - Female
MH  - Glucose/*metabolism
MH  - *Lactation/physiology
MH  - Mastitis, Bovine/immunology/metabolism/microbiology/*physiopathology
MH  - Milk/chemistry/cytology
MH  - Milk Proteins/analysis
PMC - PMC7296043
COIS- The authors declare no competing interests.
EDAT- 2020/06/17 06:00
MHDA- 2020/12/15 06:00
PMCR- 2020/06/15
CRDT- 2020/06/17 06:00
PHST- 2019/11/10 00:00 [received]
PHST- 2020/05/13 00:00 [accepted]
PHST- 2020/06/17 06:00 [entrez]
PHST- 2020/06/17 06:00 [pubmed]
PHST- 2020/12/15 06:00 [medline]
PHST- 2020/06/15 00:00 [pmc-release]
AID - 10.1038/s41598-020-66612-7 [pii]
AID - 66612 [pii]
AID - 10.1038/s41598-020-66612-7 [doi]
PST - epublish
SO  - Sci Rep. 2020 Jun 15;10(1):9602. doi: 10.1038/s41598-020-66612-7.