PMID- 32542810
OWN - NLM
STAT- MEDLINE
DCOM- 20210622
LR  - 20210622
IS  - 1440-1827 (Electronic)
IS  - 1320-5463 (Linking)
VI  - 70
IP  - 9
DP  - 2020 Sep
TI  - Genomic-based ancillary assays offer improved diagnostic yield of effusion 
      cytology with potential challenges in malignant pleural mesothelioma.
PG  - 671-679
LID - 10.1111/pin.12973 [doi]
AB  - BRCA1-associated protein 1 (BAP1) or methylthioadenosine phosphorylase (MTAP) 
      immunohistochemistry (IHC) or 9p21 fluorescence in situ hybridization (FISH) are 
      useful for the diagnosis of malignant pleural mesothelioma (MPM). However, the 
      effect of these assays on the diagnostic yield of effusion cytology in MPM cases 
      with suspicious cytomorphology or the diagnostic challenges in BAP1 or MTAP IHC 
      have not been fully elucidated. Two cohorts of cytologic preparations obtained 
      from pleural effusions were examined: MPM cases in cohort 1 were used to evaluate 
      whether BAP1 or MTAP IHC or 9p21 FISH increase the diagnostic yield of effusion 
      cytology; cohort 2 included cases suspicious for MPM, to which BAP1 or MTAP IHC 
      was applied to clarify the challenges in the clinical assessment of these assays. 
      In cohort 1 (n = 28), either assay elevated 62.5% of class II or III cases to 
      class V. In cohort 2 (n = 139), 21.7% of BAP1 immunocytochemistry in smears and 
      10.6% of BAP1 IHC and 9.4% of MTAP IHC in cell blocks, were identified to be 
      challenging. The application of genomic-based assays increased the diagnostic 
      yield of effusion cytology in the diagnosis of MPM. However, diagnostic 
      challenges limit the application of these assays in some cases.
CI  - (c) 2020 Japanese Society of Pathology and John Wiley & Sons Australia, Ltd.
FAU - Kinoshita, Yoshiaki
AU  - Kinoshita Y
AD  - Department of Pathology, Fukuoka University Hospital and School of Medicine, 
      Fukuoka, Japan.
AD  - Department of Respiratory Medicine, Fukuoka University Hospital and School of 
      Medicine, Fukuoka, Japan.
FAU - Hamasaki, Makoto
AU  - Hamasaki M
AUID- ORCID: 0000-0002-2481-3888
AD  - Department of Pathology, Fukuoka University Hospital and School of Medicine, 
      Fukuoka, Japan.
FAU - Matsumoto, Shinji
AU  - Matsumoto S
AD  - Department of Pathology, Fukuoka University Hospital and School of Medicine, 
      Fukuoka, Japan.
FAU - Yoshimura, Masayo
AU  - Yoshimura M
AD  - Department of Pathology, Fukuoka University Hospital and School of Medicine, 
      Fukuoka, Japan.
FAU - Sato, Ayuko
AU  - Sato A
AD  - Department of Pathology, Hyogo College of Medicine, Hyogo, Japan.
FAU - Tsujimura, Tohru
AU  - Tsujimura T
AD  - Department of Pathology, Hyogo College of Medicine, Hyogo, Japan.
FAU - Kamei, Toshiaki
AU  - Kamei T
AD  - Pathology and Cytology Center, BML group PCL Japan, Inc., Fukuoka, Japan.
FAU - Kawahara, Kunimitsu
AU  - Kawahara K
AUID- ORCID: 0000-0001-9103-6719
AD  - Department of Pathology, Osaka Habikino Medical Center, Osaka, Japan.
FAU - Nabeshima, Kazuki
AU  - Nabeshima K
AUID- ORCID: 0000-0002-0691-1222
AD  - Department of Pathology, Fukuoka University Hospital and School of Medicine, 
      Fukuoka, Japan.
LA  - eng
GR  - #171032/Central Research Institute of Fukuoka University/
GR  - Grants-in-Aid for Scientific Research (#24590495)/Japanese Society for the 
      Promotion of Science/
GR  - Research Center for Advanced Molecular Medicine, Fukuoka University/
PT  - Journal Article
DEP - 20200615
PL  - Australia
TA  - Pathol Int
JT  - Pathology international
JID - 9431380
RN  - 0 (BAP1 protein, human)
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Tumor Suppressor Proteins)
RN  - EC 2.4.2.1 (Purine-Nucleoside Phosphorylase)
RN  - EC 2.4.2.28 (5'-methylthioadenosine phosphorylase)
RN  - EC 3.4.19.12 (Ubiquitin Thiolesterase)
SB  - IM
MH  - Biomarkers, Tumor/chemistry/metabolism
MH  - Cytodiagnosis
MH  - Diagnosis, Differential
MH  - Genome, Human
MH  - Genomics
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization, Fluorescence
MH  - Lung Neoplasms/diagnosis/genetics/pathology
MH  - Mesothelioma/diagnosis/genetics/pathology
MH  - *Mesothelioma, Malignant/diagnosis/genetics/pathology
MH  - Neoplasm Grading
MH  - Pleural Effusion/pathology
MH  - *Pleural Neoplasms/diagnosis/genetics/pathology
MH  - *Purine-Nucleoside Phosphorylase/chemistry/genetics/metabolism
MH  - *Tumor Suppressor Proteins/chemistry/genetics/metabolism
MH  - *Ubiquitin Thiolesterase/chemistry/genetics/metabolism
OTO - NOTNLM
OT  - BRCA1-associated protein 1
OT  - cell block
OT  - methylthioadenosine phosphorylase
OT  - pleural effusion
OT  - smear
EDAT- 2020/06/17 06:00
MHDA- 2021/06/23 06:00
CRDT- 2020/06/17 06:00
PHST- 2020/03/03 00:00 [received]
PHST- 2020/05/22 00:00 [revised]
PHST- 2020/05/27 00:00 [accepted]
PHST- 2020/06/17 06:00 [pubmed]
PHST- 2021/06/23 06:00 [medline]
PHST- 2020/06/17 06:00 [entrez]
AID - 10.1111/pin.12973 [doi]
PST - ppublish
SO  - Pathol Int. 2020 Sep;70(9):671-679. doi: 10.1111/pin.12973. Epub 2020 Jun 15.