PMID- 32543628
OWN - NLM
STAT- MEDLINE
DCOM- 20210514
LR  - 20231112
IS  - 2047-4849 (Electronic)
IS  - 2047-4830 (Print)
IS  - 2047-4830 (Linking)
VI  - 8
IP  - 14
DP  - 2020 Jul 21
TI  - Customized hydrogel substrates for serum-free expansion of functional hMSCs.
PG  - 3819-3829
LID - 10.1039/d0bm00540a [doi]
AB  - We describe a screening approach to identify customized substrates for serum-free 
      human mesenchymal stromal cell (hMSC) culture. In particular, we combine a 
      biomaterials screening approach with design of experiments (DOE) and multivariate 
      analysis (MVA) to understand the effects of substrate stiffness, substrate 
      adhesivity, and media composition on hMSC behavior in vitro. This approach 
      enabled identification of poly(ethylene glycol)-based and integrin binding 
      hydrogel substrate compositions that supported functional hMSC expansion in 
      multiple serum-containing and serum-free media, as well as the expansion of MSCs 
      from multiple, distinct sources. The identified substrates were compatible with 
      standard thaw, seed, and harvest protocols. Finally, we used MVA on the screening 
      data to reveal the importance of serum and substrate stiffness on hMSC expansion, 
      highlighting the need for customized cell culture substrates in optimal hMSC 
      biomanufacturing processes.
FAU - Le, Ngoc Nhi T
AU  - Le NNT
AD  - Materials Science Program, University of Wisconsin-Madison, Madison, WI, USA. 
      wlmurphy@wisc.edu.
FAU - Liu, Tianran Leona
AU  - Liu TL
FAU - Johnston, James
AU  - Johnston J
FAU - Krutty, John D
AU  - Krutty JD
FAU - Templeton, Kayla Marie
AU  - Templeton KM
FAU - Harms, Victoria
AU  - Harms V
FAU - Dias, Andrew
AU  - Dias A
FAU - Le, Hau
AU  - Le H
FAU - Gopalan, Padma
AU  - Gopalan P
FAU - Murphy, William L
AU  - Murphy WL
LA  - eng
GR  - R01 HL093282/HL/NHLBI NIH HHS/United States
PT  - Journal Article
DEP - 20200616
PL  - England
TA  - Biomater Sci
JT  - Biomaterials science
JID - 101593571
RN  - 0 (Culture Media)
RN  - 0 (Hydrogels)
SB  - IM
MH  - Cell Culture Techniques
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Culture Media
MH  - Humans
MH  - Hydrogels
MH  - *Mesenchymal Stem Cells
PMC - PMC7436193
MID - NIHMS1605150
COIS- Conflicts of interest
EDAT- 2020/06/17 06:00
MHDA- 2021/05/15 06:00
PMCR- 2021/07/21
CRDT- 2020/06/17 06:00
PHST- 2020/06/17 06:00 [pubmed]
PHST- 2021/05/15 06:00 [medline]
PHST- 2020/06/17 06:00 [entrez]
PHST- 2021/07/21 00:00 [pmc-release]
AID - 10.1039/d0bm00540a [doi]
PST - ppublish
SO  - Biomater Sci. 2020 Jul 21;8(14):3819-3829. doi: 10.1039/d0bm00540a. Epub 2020 Jun 
      16.