PMID- 32546357
OWN - NLM
STAT- MEDLINE
DCOM- 20201215
LR  - 20201215
IS  - 1532-2971 (Electronic)
IS  - 1090-0233 (Linking)
VI  - 257
DP  - 2020 Mar
TI  - Carnitine palmitoyltransferase 1 A expression profile in canine mammary tumors.
PG  - 105453
LID - S1090-0233(20)30030-7 [pii]
LID - 10.1016/j.tvjl.2020.105453 [doi]
AB  - Genetic alterations and/or epigenetic modifications occur frequently in the 
      majority of cancer cells. In addition to playing a crucial role as promoters of 
      tumorigenesis, these processes can also generate metabolic pathways that are 
      different from those in normal cells. Besides the Warburg effect, an alteration 
      in lipid metabolism is also found in cancer cells. Thus, elucidation of the 
      regulators involved in this metabolic reprogramming might provide tools for 
      diagnosis, prognosis, and ultimately treatment of canine mammary tumours (CMTs) 
      in particular. One such regulator is carnitine palmitoyltransferase 1A (CPT1A), 
      which is involved in transportation of long-chain fatty acids into the 
      mitochondrial matrix for beta-oxidation, thereby providing an alternative pathway 
      for the generation of energy for tumour growth and development. In this study, 
      the canine cell lines MDCK, CMT-U309, CMT-U27, and P114 were used as in vitro 
      models for western blot and quantitative real-time polymerase chain reaction 
      (qRT-PCR) analyses. Furthermore, western blot and immunohistochemistry were 
      carried out to evaluate CPT1A protein expression in the CMT specimens. The CPT1A 
      protein and mRNA expression levels were increased in the CMT cell lines relative 
      to their levels in normal epithelial cells. Moreover, increased CPT1A expression 
      levels were found in the CMT tissues, being inversely correlated with the tumour 
      differentiation grade. However, additional studies are required to further 
      specify the role of CPT1A in CMTs.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Cacciola, Nunzio Antonio
AU  - Cacciola NA
AD  - Department of Veterinary Medicine and Animal Productions, University of Naples 
      "Federico II", Via F. Delpino 1, Naples, Italy; Institute of Sustainable Plant 
      Protection (IPSP), National Research Council (CNR), Via Universita, 111, Naples, 
      Italy; Research Institute on Terrestrial Ecosystems (IRET), National Research 
      Council (CNR), Via P. Castellino, 111, Naples, Italy. Electronic address: 
      nunzioantonio.cacciola@unina.it.
FAU - Sgadari, Mariafrancesca
AU  - Sgadari M
AD  - Department of Veterinary Medicine and Animal Productions, University of Naples 
      "Federico II", Via F. Delpino 1, Naples, Italy.
FAU - Petillo, Orsolina
AU  - Petillo O
AD  - Research Institute on Terrestrial Ecosystems (IRET), National Research Council 
      (CNR), Via P. Castellino, 111, Naples, Italy.
FAU - Margarucci, Sabrina
AU  - Margarucci S
AD  - Research Institute on Terrestrial Ecosystems (IRET), National Research Council 
      (CNR), Via P. Castellino, 111, Naples, Italy.
FAU - Martano, Manuela
AU  - Martano M
AD  - Department of Veterinary Medicine and Animal Productions, University of Naples 
      "Federico II", Via F. Delpino 1, Naples, Italy.
FAU - Cocchia, Natascia
AU  - Cocchia N
AD  - Department of Veterinary Medicine and Animal Productions, University of Naples 
      "Federico II", Via F. Delpino 1, Naples, Italy.
FAU - Maiolino, Paola
AU  - Maiolino P
AD  - Department of Veterinary Medicine and Animal Productions, University of Naples 
      "Federico II", Via F. Delpino 1, Naples, Italy.
FAU - Restucci, Brunella
AU  - Restucci B
AD  - Department of Veterinary Medicine and Animal Productions, University of Naples 
      "Federico II", Via F. Delpino 1, Naples, Italy.
LA  - eng
PT  - Journal Article
DEP - 20200403
PL  - England
TA  - Vet J
JT  - Veterinary journal (London, England : 1997)
JID - 9706281
RN  - EC 2.3.1.21 (Carnitine O-Palmitoyltransferase)
SB  - IM
MH  - Animals
MH  - Blotting, Western/veterinary
MH  - Carnitine O-Palmitoyltransferase/*genetics/metabolism
MH  - Cell Line, Tumor
MH  - Dog Diseases/*genetics/metabolism
MH  - Dogs
MH  - Female
MH  - Immunohistochemistry/veterinary
MH  - Madin Darby Canine Kidney Cells
MH  - Mammary Neoplasms, Animal/*genetics/metabolism
MH  - *Transcriptome
OTO - NOTNLM
OT  - Canine mammary tumors
OT  - Fatty acid beta-oxidation
OT  - Immunohistochemistry
OT  - Metabolism
OT  - Mitochondria
EDAT- 2020/06/18 06:00
MHDA- 2020/12/16 06:00
CRDT- 2020/06/18 06:00
PHST- 2019/07/22 00:00 [received]
PHST- 2020/03/26 00:00 [revised]
PHST- 2020/03/27 00:00 [accepted]
PHST- 2020/06/18 06:00 [entrez]
PHST- 2020/06/18 06:00 [pubmed]
PHST- 2020/12/16 06:00 [medline]
AID - S1090-0233(20)30030-7 [pii]
AID - 10.1016/j.tvjl.2020.105453 [doi]
PST - ppublish
SO  - Vet J. 2020 Mar;257:105453. doi: 10.1016/j.tvjl.2020.105453. Epub 2020 Apr 3.