PMID- 32547144
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220415
IS  - 1178-7007 (Print)
IS  - 1178-7007 (Electronic)
IS  - 1178-7007 (Linking)
VI  - 13
DP  - 2020
TI  - Genetic Association of SH2B1 Gene Polymorphisms in Jordanian Arab Patients with 
      Type 2 Diabetes Mellitus.
PG  - 1825-1834
LID - 10.2147/DMSO.S245843 [doi]
AB  - OBJECTIVE: To investigate the genotypic and allelic association of Src homology 2 
      B adapter protein 1 (SH2B1) gene polymorphisms with type 2 diabetes mellitus 
      (T2DM) in Jordanian patients. PATIENTS AND METHODS: Three hundred patients were 
      screened, but only 200 adult Jordanian patients diagnosed with T2DM (53.5% male 
      and 46.5% female) have participated in this study. Blood samples were collected 
      from both patients and healthy individuals for DNA extraction according to 
      well-established procedures. Exon 1 and exon 9 of the SH2B1 gene were sequenced 
      using an efficient and sensitive DNA sequencing method in order to identify 
      specific single nucleotide polymorphisms (SNPs) in the SH2B1 gene associated with 
      T2DM. Genetic and haplotype correlation analysis was performed for the chosen 
      SNPs to detect any association if existent. In addition, SNPStats Web Tool and 
      Hardy-Weinberg equilibrium (HWE) analyses for the genotype distribution were 
      used. The significance was determined according to the P-value, and the level of 
      significance taken as P < 0.05. The normality of the data distribution was 
      statically analysed by the Shapiro-Wilk test with a P-value >0.05. Also, the 
      patient's characteristics and clinical data about all participants were 
      mentioned. RESULTS: Two novel variations were present in the SH2B1 gene in 
      Jordanian patients with T2DM: c.827C>G and c.2026G>A, and previously reported 
      five SNPs: rs146946750, rs565131715, rs370302573, rs143212778, rs200470848. Our 
      results showed a strong genetic association of rs565131715 SNP polymorphism 
      within the SH2B1 gene in T2DM patients (chi (2) test, P < 0.001). Additionally, 
      rs143212778 SNP presented a genetic correlation with T2DM patients (chi (2) test, P 
      = 0.035) as compared to control individuals. GTACG haplotype of SH2B1 has a 
      highly significant association with responders (P< 0.0001). CONCLUSION: Our 
      findings indicated a strong association between the rs565131715 polymorphism and 
      the risk of T2DM among the Jordanian population. Moreover, our data showed that 
      the rs143212778 polymorphism significantly elevated the danger of T2DM among this 
      population. This study reveals the first data regarding the SH2B1 gene 
      polymorphisms in Jordanian patients of Arab descent with diabetes.
CI  - (c) 2020 AL-Eitan et al.
FAU - Al-Eitan, Laith N
AU  - Al-Eitan LN
AUID- ORCID: 0000-0003-0064-0190
AD  - Department of Applied Biological Sciences, Jordan University of Science and 
      Technology, Irbid 22110, Jordan.
AD  - Department of Biotechnology and Genetic Engineering, Jordan University of Science 
      and Technology, Irbid 22110, Jordan.
FAU - Aman, Hatem
AU  - Aman H
AD  - Department of Biotechnology and Genetic Engineering, Jordan University of Science 
      and Technology, Irbid 22110, Jordan.
FAU - Alkhatib, Rami
AU  - Alkhatib R
AUID- ORCID: 0000-0003-1635-8624
AD  - Department of Applied Biological Sciences, Jordan University of Science and 
      Technology, Irbid 22110, Jordan.
AD  - Department of Biotechnology and Genetic Engineering, Jordan University of Science 
      and Technology, Irbid 22110, Jordan.
FAU - Alghamdi, Mansour A
AU  - Alghamdi MA
AUID- ORCID: 0000-0002-6613-1498
AD  - College of Medicine, King Khalid University, Abha 61421, Saudi Arabia.
LA  - eng
PT  - Journal Article
DEP - 20200522
PL  - New Zealand
TA  - Diabetes Metab Syndr Obes
JT  - Diabetes, metabolic syndrome and obesity : targets and therapy
JID - 101515585
PMC - PMC7250702
OTO - NOTNLM
OT  - Arab
OT  - Jordan
OT  - SH2B1
OT  - type 2 diabetes mellitus
COIS- The authors report no conflicts of interest in this work.
EDAT- 2020/06/18 06:00
MHDA- 2020/06/18 06:01
PMCR- 2020/05/22
CRDT- 2020/06/18 06:00
PHST- 2020/01/13 00:00 [received]
PHST- 2020/05/05 00:00 [accepted]
PHST- 2020/06/18 06:00 [entrez]
PHST- 2020/06/18 06:00 [pubmed]
PHST- 2020/06/18 06:01 [medline]
PHST- 2020/05/22 00:00 [pmc-release]
AID - 245843 [pii]
AID - 10.2147/DMSO.S245843 [doi]
PST - epublish
SO  - Diabetes Metab Syndr Obes. 2020 May 22;13:1825-1834. doi: 10.2147/DMSO.S245843. 
      eCollection 2020.