PMID- 32548117
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 2296-634X (Print)
IS  - 2296-634X (Electronic)
IS  - 2296-634X (Linking)
VI  - 8
DP  - 2020
TI  - Label-Free Infrared Spectral Histology of Skin Tissue Part II: Impact of a 
      Lumican-Derived Peptide on Melanoma Growth.
PG  - 377
LID - 10.3389/fcell.2020.00377 [doi]
LID - 377
AB  - Melanoma is the most aggressive type of cutaneous malignancies. In addition to 
      its role as a regulator of extracellular matrix (ECM) integrity, lumican, a small 
      leucine-rich proteoglycan, also exhibits anti-tumor properties in melanoma. This 
      work focuses on the use of infrared spectral imaging (IRSI) and histopathology 
      (IRSH) to study the effect of lumican-derived peptide (L9Mc) on B16F1 melanoma 
      primary tumor growth. Female C57BL/6 mice were injected with B16F1 cells treated 
      with L9Mc (n = 10) or its scrambled peptide (n = 8), and without peptide 
      (control, n = 9). The melanoma primary tumors were subjected to histological and 
      IR imaging analysis. In addition, immunohistochemical staining was performed 
      using anti-Ki-67 and anti-cleaved caspase-3 antibodies. The IR images were 
      analyzed by common K-means clustering to obtain high-contrast IRSH that allowed 
      identifying different ECM tissue regions from the epidermis to the tumor area, 
      which correlated well with H&E staining. Furthermore, IRSH showed good 
      correlation with immunostaining data obtained with anti-Ki-67 and anti-cleaved 
      caspase-3 antibodies, whereby the L9Mc peptide inhibited cell proliferation and 
      increased strongly apoptosis of B16F1 cells in this mouse model of melanoma 
      primary tumors.
CI  - Copyright (c) 2020 Brezillon, Untereiner, Mohamed, Ahallal, Proult, Nizet, 
      Boulagnon-Rombi and Sockalingum.
FAU - Brezillon, Stephane
AU  - Brezillon S
AD  - Universite de Reims Champagne-Ardenne, Laboratoire de Biochimie Medicale et 
      Biologie Moleculaire, Reims, France.
AD  - CNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire - MEDyC, Reims, 
      France.
FAU - Untereiner, Valerie
AU  - Untereiner V
AD  - Universite de Reims Champagne-Ardenne, PICT, Reims, France.
FAU - Mohamed, Hossam Taha
AU  - Mohamed HT
AD  - Universite de Reims Champagne-Ardenne, Laboratoire de Biochimie Medicale et 
      Biologie Moleculaire, Reims, France.
AD  - CNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire - MEDyC, Reims, 
      France.
AD  - Zoology Department, Faculty of Science, Cairo University, Giza, Egypt.
AD  - Faculty of Biotechnology, October University for Modern Sciences and Arts, Giza, 
      Egypt.
FAU - Ahallal, Estelle
AU  - Ahallal E
AD  - Universite de Reims Champagne-Ardenne, Laboratoire de Biochimie Medicale et 
      Biologie Moleculaire, Reims, France.
AD  - CNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire - MEDyC, Reims, 
      France.
FAU - Proult, Isabelle
AU  - Proult I
AD  - Universite de Reims Champagne-Ardenne, Laboratoire de Biochimie Medicale et 
      Biologie Moleculaire, Reims, France.
AD  - CNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire - MEDyC, Reims, 
      France.
FAU - Nizet, Pierre
AU  - Nizet P
AD  - Universite de Reims Champagne-Ardenne, Laboratoire de Biochimie Medicale et 
      Biologie Moleculaire, Reims, France.
AD  - CNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire - MEDyC, Reims, 
      France.
FAU - Boulagnon-Rombi, Camille
AU  - Boulagnon-Rombi C
AD  - CNRS UMR 7369, Matrice Extracellulaire et Dynamique Cellulaire - MEDyC, Reims, 
      France.
AD  - CHU de Reims, Laboratoire Central d'Anatomie et de Cytologie Pathologique, Reims, 
      France.
FAU - Sockalingum, Ganesh D
AU  - Sockalingum GD
AD  - Universite de Reims Champagne-Ardenne, BioSpecT-EA7506, Reims, France.
LA  - eng
PT  - Journal Article
DEP - 20200529
PL  - Switzerland
TA  - Front Cell Dev Biol
JT  - Frontiers in cell and developmental biology
JID - 101630250
PMC - PMC7273845
OTO - NOTNLM
OT  - B16F1
OT  - immunohistochemistry
OT  - infrared histology
OT  - lumican-derived peptides
OT  - melanoma
EDAT- 2020/06/18 06:00
MHDA- 2020/06/18 06:01
PMCR- 2020/01/01
CRDT- 2020/06/18 06:00
PHST- 2020/02/11 00:00 [received]
PHST- 2020/04/27 00:00 [accepted]
PHST- 2020/06/18 06:00 [entrez]
PHST- 2020/06/18 06:00 [pubmed]
PHST- 2020/06/18 06:01 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - 10.3389/fcell.2020.00377 [doi]
PST - epublish
SO  - Front Cell Dev Biol. 2020 May 29;8:377. doi: 10.3389/fcell.2020.00377. 
      eCollection 2020.