PMID- 32549371
OWN - NLM
STAT- MEDLINE
DCOM- 20210216
LR  - 20231111
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 25
IP  - 12
DP  - 2020 Jun 15
TI  - Enhanced Stability of Lipid Structures by Dip-Pen Nanolithography on Block-Type 
      MPC Copolymer.
LID - 10.3390/molecules25122768 [doi]
LID - 2768
AB  - Biomimetic lipid membranes on solid supports have been used in a plethora of 
      applications, including as biosensors, in research on membrane proteins or as 
      interfaces in cell experiments. For many of these applications, structured lipid 
      membranes, e.g., in the form of arrays with features of different functionality, 
      are highly desired. The stability of these features on a given substrate during 
      storage and in incubation steps is key, while at the same time the substrate 
      ideally should also exhibit antifouling properties. Here, we describe the highly 
      beneficial properties of a 2-methacryloyloxyethyl phosphorylcholine (MPC) 
      copolymer for the stability of supported lipid membrane structures generated by 
      dip-pen nanolithography with phospholipids (L-DPN). The MPC copolymer substrates 
      allow for more stable and higher membrane stack structures in comparison to other 
      hydrophilic substrates, like glass or silicon oxide surfaces. The structures 
      remain highly stable under immersion in liquid and subsequent incubation and 
      washing steps. This allows multiplexed functionalization of lipid arrays with 
      antibodies via microchannel cantilever spotting (microCS), without the need of 
      orthogonal binding tags for each antibody type. The combined properties of the 
      MPC copolymer substrate demonstrate a great potential for lipid-based biomedical 
      sensing and diagnostic platforms.
FAU - Liu, Hui-Yu
AU  - Liu HY
AD  - Institute of Nanotechnology (INT) & Karlsruhe Nano Micro Facility (KNMF), 
      Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344 
      Eggenstein-Leopoldshafen, Germany.
FAU - Kumar, Ravi
AU  - Kumar R
AUID- ORCID: 0000-0002-7099-6865
AD  - Institute of Nanotechnology (INT) & Karlsruhe Nano Micro Facility (KNMF), 
      Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344 
      Eggenstein-Leopoldshafen, Germany.
FAU - Takai, Madoka
AU  - Takai M
AD  - Department of Bioengineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-Ku, 
      Tokyo 113-8656, Japan.
FAU - Hirtz, Michael
AU  - Hirtz M
AUID- ORCID: 0000-0002-2647-5317
AD  - Institute of Nanotechnology (INT) & Karlsruhe Nano Micro Facility (KNMF), 
      Karlsruhe Institute of Technology (KIT), Hermann-von-Helmholtz-Platz 1, 76344 
      Eggenstein-Leopoldshafen, Germany.
LA  - eng
PT  - Journal Article
DEP - 20200615
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Membrane Lipids)
RN  - 0 (Membranes, Artificial)
RN  - 0 (Methacrylates)
RN  - 0 (Phospholipids)
RN  - 0 (Polymers)
RN  - 107-73-3 (Phosphorylcholine)
RN  - 59RU860S8D (2-methacryloyloxyethyl phosphorylcholine)
RN  - 7631-86-9 (Silicon Dioxide)
SB  - IM
MH  - Biomimetic Materials/chemistry
MH  - Membrane Lipids/chemical synthesis/*chemistry
MH  - *Membranes, Artificial
MH  - Methacrylates/*chemistry
MH  - Microscopy, Atomic Force/methods
MH  - Nanotechnology/methods
MH  - Phospholipids/chemistry
MH  - Phosphorylcholine/*analogs & derivatives/chemistry
MH  - Polymers/chemistry
MH  - Silicon Dioxide/chemistry
PMC - PMC7356513
OTO - NOTNLM
OT  - MPC copolymer
OT  - lipid dip-pen nanolithography
OT  - microchannel cantilever spotting
OT  - phospholipids
COIS- The authors declare no conflict of interest.
EDAT- 2020/06/19 06:00
MHDA- 2021/02/17 06:00
PMCR- 2020/06/15
CRDT- 2020/06/19 06:00
PHST- 2020/05/14 00:00 [received]
PHST- 2020/06/09 00:00 [revised]
PHST- 2020/06/12 00:00 [accepted]
PHST- 2020/06/19 06:00 [entrez]
PHST- 2020/06/19 06:00 [pubmed]
PHST- 2021/02/17 06:00 [medline]
PHST- 2020/06/15 00:00 [pmc-release]
AID - molecules25122768 [pii]
AID - molecules-25-02768 [pii]
AID - 10.3390/molecules25122768 [doi]
PST - epublish
SO  - Molecules. 2020 Jun 15;25(12):2768. doi: 10.3390/molecules25122768.