PMID- 32549781
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220415
IS  - 1452-8258 (Print)
IS  - 1452-8266 (Electronic)
IS  - 1452-8266 (Linking)
VI  - 39
IP  - 1
DP  - 2020 Jan 10
TI  - Urinary nephrin is earlier, more sensitive and specific marker of diabetic 
      nephropathy than microalbuminuria.
PG  - 83-90
LID - 10.2478/jomb-2019-0026 [doi]
AB  - BACKGROUND: Diabetic nephropathy (DN) is a leading cause of end-stage renal 
      disease. Progressive damage and decline in the number of podocytes often occur in 
      the early stages of DN. Thus, nephrin as a podocyte-specific protein may be 
      regarded as a potential biomarker of early detection of DN. The aim of this study 
      is to determine whether urinary nephrin is an earlier marker in DN than 
      microalbuminuria and to test the significance of urinary nephrin as a marker for 
      early detection of DN. METHODS: Our cross-sectional study included 90 patients 
      with type 2 diabetes mellitus (T2DM), 30 patients with diagnosed DN and 60 
      patients without diagnosed DN. As a control group, we used 30 healthy subjects. 
      All patients with T2DM were classified into three subgroups according to urinary 
      microalbumin/creatinine ratio (UMCR): normoalbuminuric, microalbuminuric and 
      macroalbuminuric patients. Nephrin in urine was measured by immunoenzyme assay, 
      microalbumin with turbidimetric and creatinine with the photometric method. In 
      blood sera, we measured a few standard biochemical parameters. RESULTS: 
      Nephrinuria was found to be present in 100% of patients with T2DM and 
      macroalbuminuria, in 88% with microalbuminuria, as well as 82% of patients with 
      T2DM and normoalbuminuria. A concentration of urinary nephrin was significantly 
      increased in all groups of subjects with T2DM compared to the control group 
      (p<0.05). Nephrinuria correlated statistically negative with eGFR (r=-0.54). ROC 
      analysis showed that nephrin has a total predicted probability of 96% in patients 
      with DN. CONCLUSIONS: Urinary nephrin is earlier, more specific and sensitive 
      marker than microalbumin in early detection of DN.
CI  - 2020 Irena Kostovska, Katerina Tosheska-Trajkovska, Sonja Topuzovska, Svetlana 
      Cekovska, Goce Spasovski, Ognen Kostovski, Danica Labudovic, published by 
      CEON/CEES.
FAU - Kostovska, Irena
AU  - Kostovska I
AD  - Medical Faculty, Department of Medical and Experimental Biochemistry, Skopje, R. 
      Macedonia.
FAU - Tosheska-Trajkovska, Katerina
AU  - Tosheska-Trajkovska K
AD  - Medical Faculty, Department of Medical and Experimental Biochemistry, Skopje, R. 
      Macedonia.
FAU - Topuzovska, Sonja
AU  - Topuzovska S
AD  - Medical Faculty, Department of Medical and Experimental Biochemistry, Skopje, R. 
      Macedonia.
FAU - Cekovska, Svetlana
AU  - Cekovska S
AD  - Medical Faculty, Department of Medical and Experimental Biochemistry, Skopje, R. 
      Macedonia.
FAU - Spasovski, Goce
AU  - Spasovski G
AD  - Medical Faculty, University Department of Nephrology, Skopje, R. Macedonia.
FAU - Kostovski, Ognen
AU  - Kostovski O
AD  - Medical Faculty, University Clinic of Abdominal Surgery, Skopje, R. Macedonia.
FAU - Labudovic, Danica
AU  - Labudovic D
AD  - Medical Faculty, Department of Medical and Experimental Biochemistry, Skopje, R. 
      Macedonia.
LA  - eng
PT  - Journal Article
PL  - Serbia
TA  - J Med Biochem
JT  - Journal of medical biochemistry
JID - 101315490
PMC - PMC7282240
OTO - NOTNLM
OT  - diabetic nephropathy
OT  - microalbumin
OT  - nephrin
COIS- Conflict of Interest: The authors stated that they have no conflicts of interest 
      regarding the publication of this article.
EDAT- 2020/06/19 06:00
MHDA- 2020/06/19 06:01
PMCR- 2020/01/10
CRDT- 2020/06/19 06:00
PHST- 2019/03/18 00:00 [received]
PHST- 2019/04/29 00:00 [accepted]
PHST- 2020/06/19 06:00 [entrez]
PHST- 2020/06/19 06:00 [pubmed]
PHST- 2020/06/19 06:01 [medline]
PHST- 2020/01/10 00:00 [pmc-release]
AID - jomb-39-1-2001083K [pii]
AID - 10.2478/jomb-2019-0026 [doi]
PST - ppublish
SO  - J Med Biochem. 2020 Jan 10;39(1):83-90. doi: 10.2478/jomb-2019-0026.