PMID- 32550885
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 20
IP  - 1
DP  - 2020 Jul
TI  - Raloxifene improves TNF-alpha-induced osteogenic differentiation inhibition of bone 
      marrow mesenchymal stem cells and alleviates osteoporosis.
PG  - 309-314
LID - 10.3892/etm.2020.8689 [doi]
AB  - Effect of raloxifene (RLF) on the improvement of inhibited osteogenic 
      differentiation of bone marrow mesenchymal stem cells (BMSCs) resulted from tumor 
      necrosis factor-alpha (TNF-alpha) induction, thus alleviating the progression of 
      osteoporosis (OP), was investigated. An in vivo OP rat model was constructed by 
      performing the procedures of ovariectomy (OVX). Rats were randomly divided into 
      sham group, OVX group and RLF+OVX group. BMSCs were extracted from healthy rats, 
      and randomly divided into control group, TNF-alpha group, RLF group and TNF-alpha+RLF 
      group. Viability and cellular calcification ability in each group were detected. 
      The relative levels of osteocalcin (OCN), Runx2 and NF-kappaB in cells with different 
      treatments were determined. The body weight of rats in the OVX group and RLF+OVX 
      group gradually increased compared with that in the sham group on the 8th week. 
      No significant difference in body weight was observed between the rats of the OVX 
      group and RLF+OVX group. Bone metabolism index (BMD) in the rats of the RLF+OVX 
      group was higher than that of the OVX group, and lower compared with that of the 
      sham group. Compared with the sham group, the elastic/max radial degree and 
      elastic/max load of femora were reduced in the OVX group and RLF+OVX group, 
      especially in the OVX group. The relative levels of OCN and Runx2, as well as the 
      ALP activity and calcification ability, were decreased in the OVX group compared 
      with the sham group, and the effect was partially reversed by the RLF treatment. 
      After osteogenic differentiation of BMSCs, the viability and calcification 
      ability were markedly reduced in TNF-alpha group, which was reversed by RLF 
      treatment. Moreover, TNF-alpha induction downregulated the relative levels of OCN and 
      Runx2, and RLF treatment could enhance their levels. The upregulated NF-kappaB 
      protein level, induced by TNF-alpha, was reduced after RLF treatment. TNF-alpha induction 
      inhibits osteogenic differentiation of BMSCs, which could be remarkably 
      alleviated by RLF. It is suggested that RLF contributes to the alleviation of OP 
      progression.
CI  - Copyright: (c) Yang et al.
FAU - Yang, Fenghe
AU  - Yang F
AD  - Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, 
      School of Biological Science and Medical Engineering, Beihang University, Beijing 
      100083, P.R. China.
AD  - Beijing Advanced Innovation Centre for Biomedical Engineering, Beihang 
      University, Beijing 100083, P.R. China.
FAU - Jia, Yusong
AU  - Jia Y
AD  - Department of Orthopaedics, Dongzhimen Hospital, Beijing University of Chinese 
      Medicine, Beijing 100700, P.R. China.
FAU - Sun, Qi
AU  - Sun Q
AD  - Department of Orthopaedics, Dongzhimen Hospital, Beijing University of Chinese 
      Medicine, Beijing 100700, P.R. China.
FAU - Zheng, Chenying
AU  - Zheng C
AD  - Department of Orthopaedics, Dongzhimen Hospital, Beijing University of Chinese 
      Medicine, Beijing 100700, P.R. China.
FAU - Liu, Chuyin
AU  - Liu C
AD  - Department of Orthopaedics, Dongzhimen Hospital, Beijing University of Chinese 
      Medicine, Beijing 100700, P.R. China.
FAU - Wang, Wei
AU  - Wang W
AD  - Department of Orthopaedics, Dongzhimen Hospital, Beijing University of Chinese 
      Medicine, Beijing 100700, P.R. China.
FAU - Du, Li
AU  - Du L
AD  - Department of Orthopaedics, Dongzhimen Hospital, Beijing University of Chinese 
      Medicine, Beijing 100700, P.R. China.
FAU - Kang, Shengqian
AU  - Kang S
AD  - Department of Orthopaedics, Dongzhimen Hospital, Beijing University of Chinese 
      Medicine, Beijing 100700, P.R. China.
FAU - Niu, Xufeng
AU  - Niu X
AD  - Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education, 
      School of Biological Science and Medical Engineering, Beihang University, Beijing 
      100083, P.R. China.
AD  - Beijing Advanced Innovation Centre for Biomedical Engineering, Beihang 
      University, Beijing 100083, P.R. China.
FAU - Li, Jinyu
AU  - Li J
AD  - Department of Orthopaedics, Dongzhimen Hospital, Beijing University of Chinese 
      Medicine, Beijing 100700, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20200427
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC7296296
OTO - NOTNLM
OT  - TNF-alpha
OT  - osteoporosis
OT  - raloxifene
EDAT- 2020/06/20 06:00
MHDA- 2020/06/20 06:01
PMCR- 2020/04/27
CRDT- 2020/06/20 06:00
PHST- 2019/10/11 00:00 [received]
PHST- 2019/12/09 00:00 [accepted]
PHST- 2020/06/20 06:00 [entrez]
PHST- 2020/06/20 06:00 [pubmed]
PHST- 2020/06/20 06:01 [medline]
PHST- 2020/04/27 00:00 [pmc-release]
AID - ETM-0-0-8689 [pii]
AID - 10.3892/etm.2020.8689 [doi]
PST - ppublish
SO  - Exp Ther Med. 2020 Jul;20(1):309-314. doi: 10.3892/etm.2020.8689. Epub 2020 Apr 
      27.