PMID- 32553889
OWN - NLM
STAT- MEDLINE
DCOM- 20211005
LR  - 20211005
IS  - 1878-1519 (Electronic)
IS  - 1569-9048 (Linking)
VI  - 280
DP  - 2020 Sep
TI  - Activation of mu-opioid receptors differentially affects the preBotzinger Complex 
      and neighbouring regions of the respiratory network in the adult rabbit.
PG  - 103482
LID - S1569-9048(20)30140-3 [pii]
LID - 10.1016/j.resp.2020.103482 [doi]
AB  - The role of the different components of the respiratory network in the mediation 
      of opioid-induced respiratory depression is still unclear. We investigated the 
      contribution of the preBotzinger Complex (preBotC) and the neighbouring Botzinger 
      Complex (BotC) and inspiratory portion of the ventral respiratory group (iVRG) in 
      anesthetized, vagotomized, paralyzed and artificially ventilated adult rabbits 
      making use of bilateral microinjections (30-50 nl) of the mu-opioid receptor 
      agonist [D-Ala(2), N-Me-Phe(4), Gly(5)-ol]-enkephalin (DAMGO). Dose-dependent 
      effects were observed. In the preBotC and the BotC 0.1 mM DAMGO microinjections 
      caused mainly reductions in peak phrenic amplitude associated with tonic phrenic 
      activity and irregular (ataxic) patterns of breathing that were more pronounced 
      in the preBotC. Apneic effects developed at 0.5 mM. In the iVRG DAMGO provoked 
      decreases in amplitude and frequency of phrenic bursts at 0.1 mM and apnea at 0.5 
      mM. Local 5 mM naloxone reversed the apneic effects. The results imply that 
      different components of the respiratory network may contribute to opioid-induced 
      respiratory disorders.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Cinelli, Elenia
AU  - Cinelli E
AD  - Dipartimento di Medicina Sperimentale e Clinica, Sezione Scienze Fisiologiche, 
      Universita degli Studi di Firenze, Viale G.B. Morgagni 63, 50134, Firenze, Italy. 
      Electronic address: elenia.cinelli@unifi.it.
FAU - Bongianni, Fulvia
AU  - Bongianni F
AD  - Dipartimento di Medicina Sperimentale e Clinica, Sezione Scienze Fisiologiche, 
      Universita degli Studi di Firenze, Viale G.B. Morgagni 63, 50134, Firenze, Italy.
FAU - Pantaleo, Tito
AU  - Pantaleo T
AD  - Dipartimento di Medicina Sperimentale e Clinica, Sezione Scienze Fisiologiche, 
      Universita degli Studi di Firenze, Viale G.B. Morgagni 63, 50134, Firenze, Italy.
FAU - Mutolo, Donatella
AU  - Mutolo D
AD  - Dipartimento di Medicina Sperimentale e Clinica, Sezione Scienze Fisiologiche, 
      Universita degli Studi di Firenze, Viale G.B. Morgagni 63, 50134, Firenze, Italy.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200615
PL  - Netherlands
TA  - Respir Physiol Neurobiol
JT  - Respiratory physiology & neurobiology
JID - 101140022
RN  - 0 (Analgesics, Opioid)
RN  - 0 (Narcotic Antagonists)
RN  - 0 (Receptors, Opioid, mu)
RN  - 100929-53-1 (Enkephalin, Ala(2)-MePhe(4)-Gly(5)-)
RN  - 36B82AMQ7N (Naloxone)
SB  - IM
MH  - Analgesics, Opioid/*pharmacology
MH  - Animals
MH  - Apnea/chemically induced/physiopathology
MH  - Enkephalin, Ala(2)-MePhe(4)-Gly(5)-/*pharmacology
MH  - Medulla Oblongata/*drug effects/metabolism
MH  - Microinjections
MH  - Naloxone/pharmacology
MH  - Narcotic Antagonists/pharmacology
MH  - Neurons
MH  - Phrenic Nerve/*drug effects/physiopathology
MH  - Rabbits
MH  - Receptors, Opioid, mu/agonists/*metabolism
MH  - Respiratory Center/*drug effects/metabolism
MH  - Respiratory Insufficiency/*chemically induced/physiopathology
OTO - NOTNLM
OT  - Control of breathing
OT  - Medullary respiratory network
OT  - Opioid-induced respiratory depression
OT  - preBotzinger Complex
OT  - mu-Opioid receptors
EDAT- 2020/06/20 06:00
MHDA- 2021/10/06 06:00
CRDT- 2020/06/20 06:00
PHST- 2020/03/16 00:00 [received]
PHST- 2020/06/05 00:00 [revised]
PHST- 2020/06/12 00:00 [accepted]
PHST- 2020/06/20 06:00 [pubmed]
PHST- 2021/10/06 06:00 [medline]
PHST- 2020/06/20 06:00 [entrez]
AID - S1569-9048(20)30140-3 [pii]
AID - 10.1016/j.resp.2020.103482 [doi]
PST - ppublish
SO  - Respir Physiol Neurobiol. 2020 Sep;280:103482. doi: 10.1016/j.resp.2020.103482. 
      Epub 2020 Jun 15.