PMID- 32554268
OWN - NLM
STAT- MEDLINE
DCOM- 20210602
LR  - 20210602
IS  - 1873-2534 (Electronic)
IS  - 0165-2427 (Linking)
VI  - 227
DP  - 2020 Sep
TI  - Mycoplasma bovis induces matrix metalloproteinase-3 expression in bovine synovial 
      cells via up-regulation of interleukin-1beta expression in mononuclear cells.
PG  - 110057
LID - S0165-2427(20)30083-0 [pii]
LID - 10.1016/j.vetimm.2020.110057 [doi]
AB  - Mycoplasma bovis causes chronic arthritis in calves, presenting as osteolysis in 
      affected joints. Matrix metalloproteinase-3 (MMP-3), an enzyme involved in 
      cartilage degradation, is produced by synovial cells. Production of this 
      proteinase is regulated by interleukin (IL)-1beta, which is produced by mononuclear 
      cells. Both factors are known to play important roles in osteolysis in human 
      autoimmune and bacterial arthritis. However, the pathophysiology of Mycoplasma 
      arthritis (MA) has not been elucidated. In this study, we evaluated the levels of 
      MMP-3 and IL-1beta in synovial fluid (SF) from MA calves and examined the effect of 
      IL-1beta on MMP-3 expression in bovine synovial cells in vitro. Levels of MMP-3 and 
      IL-1beta in SF from MA calves were significantly higher than those of clinically 
      healthy calves. Mycoplasma bovis induced significant increases in the expression 
      of IL-1beta mRNA and protein in mononuclear cells, compared with cells not exposed 
      to M. bovis. Interestingly, the supernatant of mononuclear cells stimulated with 
      M. bovis contained high levels of IL-1beta, which induced higher expression of MMP-3 
      mRNA and protein in synovial cells than direct stimulation by M. bovis. 
      Recombinant bovine IL-1beta also induced increased MMP-3 mRNA and protein expression 
      in synovial cells. Our results indicate that M. bovis induces IL-1beta expression by 
      bovine mononuclear cells, and this cytokine then promotes MMP-3 production by 
      synovial cells. These findings suggest that MMP-3 and IL-1beta are key factors in 
      the development of osteolysis in MA calves.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Nishi, Koji
AU  - Nishi K
AD  - Animal Health Laboratory, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, 
      Japan.
FAU - Gondaira, Satoshi
AU  - Gondaira S
AD  - Animal Health Laboratory, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, 
      Japan.
FAU - Okamoto, Mariko
AU  - Okamoto M
AD  - Animal Health Laboratory, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, 
      Japan.
FAU - Watanabe, Reina
AU  - Watanabe R
AD  - Animal Health Laboratory, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, 
      Japan.
FAU - Hirano, Yuki
AU  - Hirano Y
AD  - Animal Health Laboratory, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, 
      Japan.
FAU - Fujiki, Jumpei
AU  - Fujiki J
AD  - Laboratory of Veterinary Biochemistry, Rakuno Gakuen University, Ebetsu, Hokkaido 
      069-8501, Japan.
FAU - Iwano, Hidetomo
AU  - Iwano H
AD  - Laboratory of Veterinary Biochemistry, Rakuno Gakuen University, Ebetsu, Hokkaido 
      069-8501, Japan.
FAU - Higuchi, Hidetoshi
AU  - Higuchi H
AD  - Animal Health Laboratory, Rakuno Gakuen University, Ebetsu, Hokkaido 069-8501, 
      Japan. Electronic address: higuchi@rakuno.ac.jp.
LA  - eng
PT  - Journal Article
DEP - 20200531
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Interleukin-1beta)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
EIN - Vet Immunol Immunopathol. 2021 Jan;231:110164. PMID: 33308875
MH  - Animals
MH  - Arthritis, Infectious/immunology/*veterinary
MH  - Cattle
MH  - Cattle Diseases/*immunology/microbiology
MH  - Cells, Cultured
MH  - Interleukin-1beta/*immunology
MH  - Leukocytes, Mononuclear/immunology/microbiology
MH  - Matrix Metalloproteinase 3/*genetics
MH  - Mycoplasma Infections/immunology/*veterinary
MH  - Mycoplasma bovis
MH  - Synovial Fluid/chemistry/immunology
MH  - Synovial Membrane/cytology/*immunology/microbiology
MH  - Up-Regulation
OTO - NOTNLM
OT  - Interleukin-1beta
OT  - Matrix metalloproteinase-3
OT  - Mycoplasma bovis
OT  - Osteolysis
OT  - Synovial cells
EDAT- 2020/06/20 06:00
MHDA- 2021/06/03 06:00
CRDT- 2020/06/20 06:00
PHST- 2019/11/05 00:00 [received]
PHST- 2020/04/08 00:00 [revised]
PHST- 2020/04/16 00:00 [accepted]
PHST- 2020/06/20 06:00 [pubmed]
PHST- 2021/06/03 06:00 [medline]
PHST- 2020/06/20 06:00 [entrez]
AID - S0165-2427(20)30083-0 [pii]
AID - 10.1016/j.vetimm.2020.110057 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2020 Sep;227:110057. doi: 10.1016/j.vetimm.2020.110057. 
      Epub 2020 May 31.