PMID- 32557417
OWN - NLM
STAT- MEDLINE
DCOM- 20201030
LR  - 20210701
IS  - 1833-3516 (Print)
IS  - 2209-1491 (Electronic)
IS  - 1833-3516 (Linking)
VI  - 50
IP  - 2
DP  - 2020 Jun 30
TI  - Experimental use of flow cytometry to detect bacteria viability after hyperbaric 
      oxygen exposure: Work in progress report.
PG  - 152-156
LID - 10.28920/dhm50.2.152-156 [doi]
AB  - INTRODUCTION: Hyperbaric oxygen treatment (HBOT), based on inhaling pure oxygen 
      under elevated ambient pressure, is used as adjuvant intervention to promote 
      healing in infected wounds. Despite extensive clinical evidence of beneficial 
      effects of HBOT in soft tissue infections the mechanism of action remains to be 
      elucidated. The aim of this study was to evaluate the use of flow cytometry as a 
      novel method to assess the viability of pathogenic bacteria after hyperbaric 
      oxygen (HBO) exposure. METHODS: Bacterial strains associated with soft tissues 
      infections: Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, 
      Staphylococcus aureus were exposed to oxygen at 2.8 atmospheres absolute (atm 
      abs) (283.6 kPa) pressure for 45, 90, or 120 min, then stained with propidium 
      iodide and thiazole orange and analysed by flow cytometry. RESULTS: Escherichia 
      coli and Staphylococcus aureus showed no change in viability, nor morphology, the 
      viability of Pseudomonas aeruginosa reduced in a dose-dependent manner and 
      Klebsiella pneumoniae also showed dye uptake after HBO. CONCLUSIONS: These 
      initial results, indicate diverse sensitivity of bacteria to HBO, and suggest 
      that flow cytometry can be used to monitor viability and morphological changes 
      triggered by HBO exposure in bacteria.
CI  - Copyright: This article is the copyright of the authors who grant Diving and 
      Hyperbaric Medicine a non-exclusive licence to publish the article in electronic 
      and other forms.
FAU - Rozloznik, Miroslav
AU  - Rozloznik M
AD  - Department of Biomedical Sciences, Faculty of Medicine, University of Ostrava, 
      Czech Republic.
AD  - DAN Europe, Slovensko, Slovakia.
AD  - DNB consult, s.r.o, Kezmarok, Slovakia.
AD  - Corresponding author: Dr Miroslav Rozloznik, Department of Biomedical Sciences, 
      Medical Faculty, University of Ostrava, Syllabova 19, Ostrava, Czech Republic, 
      miroslav.rozloznik@osu.cz.
FAU - Lochmanova, Alexandra
AU  - Lochmanova A
AD  - Department of Biomedical Sciences, Faculty of Medicine, University of Ostrava, 
      Czech Republic.
AD  - Public Health Institute, Ostrava, Czech Republic.
FAU - Chmelar, Dittmar
AU  - Chmelar D
AD  - Department of Biomedical Sciences, Faculty of Medicine, University of Ostrava, 
      Czech Republic.
AD  - Czech National Anaerobic Bacteria Reference Laboratory, Faculty of Medicine, 
      University of Ostrava, Czech Republic.
FAU - Hajek, Michal
AU  - Hajek M
AD  - Department of Biomedical Sciences, Faculty of Medicine, University of Ostrava, 
      Czech Republic.
AD  - Centre for Hyperbaric Medicine, City Hospital, Ostrava, Czech Republic.
FAU - Korytkova, Karin
AU  - Korytkova K
AD  - Department of Biomedical Sciences, Faculty of Medicine, University of Ostrava, 
      Czech Republic.
FAU - Cisarikova, Monika
AU  - Cisarikova M
AD  - Department of Biomedical Sciences, Faculty of Medicine, University of Ostrava, 
      Czech Republic.
LA  - eng
PT  - Journal Article
PL  - Australia
TA  - Diving Hyperb Med
JT  - Diving and hyperbaric medicine
JID - 101282742
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Bacteria
MH  - *Flow Cytometry
MH  - *Hyperbaric Oxygenation
MH  - *Microbial Viability
MH  - Oxygen
MH  - Research Report
PMC - PMC7481111
OTO - NOTNLM
OT  - Bacteria
OT  - Flow cytometry
OT  - Hyperbaric oxygen treatment
OT  - Hyperoxia
OT  - Wounds
COIS- Conflicts of interest and funding: There were no conflicts of interest declared. 
      This project was supported by SGS grants of the specific university research of 
      the Faculty of Medicine, University of Ostrava, No. SGS20/LF/2016 and 
      SGS09/LF/2018.
EDAT- 2020/06/20 06:00
MHDA- 2020/10/31 06:00
PMCR- 2021/06/30
CRDT- 2020/06/20 06:00
PHST- 2019/09/18 00:00 [received]
PHST- 2020/02/23 00:00 [accepted]
PHST- 2020/06/20 06:00 [entrez]
PHST- 2020/06/20 06:00 [pubmed]
PHST- 2020/10/31 06:00 [medline]
PHST- 2021/06/30 00:00 [pmc-release]
AID - 10.28920/dhm50.2.152-156 [doi]
PST - ppublish
SO  - Diving Hyperb Med. 2020 Jun 30;50(2):152-156. doi: 10.28920/dhm50.2.152-156.