PMID- 32557554
OWN - NLM
STAT- MEDLINE
DCOM- 20210201
LR  - 20210201
IS  - 1521-4141 (Electronic)
IS  - 0014-2980 (Linking)
VI  - 50
IP  - 11
DP  - 2020 Nov
TI  - A two-step human culture system replicates intestinal monocyte maturation 
      cascade: Conversion of tissue-like inflammatory monocytes into macrophages.
PG  - 1676-1690
LID - 10.1002/eji.202048555 [doi]
AB  - Monocyte maturation program into macrophages (MPhi) is well defined in murine gut 
      under homeostatic or inflammatory conditions. Obviously, in vivo tracking of 
      monocytes in inflamed tissues remains difficult in humans. Furthermore, in vitro 
      models fall short in generating the surrogates of transient extravasated tissue 
      inflammatory monocytes. Here, we aimed to unravel environmental cues that 
      replicated the human monocyte "waterfall" process in vitro by first, generating 
      tissue-like inflammatory monocytes, which were then shifted toward MPhi. Purified 
      CD14(+) CD16(-) monocytes, cultured with granulocyte-macrophage 
      colony-stimulating factor (GM-CSF), IFN-gamma and IL23, differentiated into CD14(+) 
      CD163(-) cells that displayed a monocyte-like morphology. In vitro generated 
      inflammatory CD14(+) CD163(-) (inflammatory monocyte-like cells) cells promoted 
      IL-1beta-dependent memory Th17 and Th17/Th1 responses, like the CD14(+) CD163(-) 
      mo-like cells that accumulate in inflamed colon of Crohn's disease patients. 
      Next, in vitro generated inflammatory monocyte-like cells converted to functional 
      CD163(+) MPhi following exposure to TGF-beta and IL10. Gene set enrichment analysis 
      further revealed a shared molecular signature between converted CD163(+) MPhi and 
      MPhi detected in various inflamed nonlymphoid and lymphoid diseased tissues. Our 
      findings propose a two-step in vitro culture that recapitulates human monocyte 
      maturation cascade in inflamed tissue. Manipulation of this process might open 
      therapeutic avenues for chronic inflammatory disorders.
CI  - (c) 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
FAU - Bsat, Marwa
AU  - Bsat M
AD  - Immunoregulation Laboratory, Centre de Recherche du Centre Hospitalier de 
      l'Universite de Montreal (CRCHUM), Montreal, Quebec, Canada.
FAU - Chapuy, Laurence
AU  - Chapuy L
AD  - Immunoregulation Laboratory, Centre de Recherche du Centre Hospitalier de 
      l'Universite de Montreal (CRCHUM), Montreal, Quebec, Canada.
FAU - Rubio, Manuel
AU  - Rubio M
AD  - Immunoregulation Laboratory, Centre de Recherche du Centre Hospitalier de 
      l'Universite de Montreal (CRCHUM), Montreal, Quebec, Canada.
FAU - Sarfati, Marika
AU  - Sarfati M
AUID- ORCID: 0000-0003-2318-0713
AD  - Immunoregulation Laboratory, Centre de Recherche du Centre Hospitalier de 
      l'Universite de Montreal (CRCHUM), Montreal, Quebec, Canada.
LA  - eng
GR  - MOP#130533/CIHR/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200629
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD163 antigen)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Transforming Growth Factor beta)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Animals
MH  - Antigens, CD/metabolism
MH  - Antigens, Differentiation, Myelomonocytic/metabolism
MH  - Cell Differentiation/physiology
MH  - Cells, Cultured
MH  - Coculture Techniques/methods
MH  - Colon/cytology/metabolism
MH  - Humans
MH  - Inflammation/metabolism/*pathology
MH  - Interleukin-10/metabolism
MH  - Lipopolysaccharide Receptors/metabolism
MH  - Macrophages/*cytology/metabolism
MH  - Mice
MH  - Monocytes/*cytology/metabolism
MH  - Receptors, Cell Surface/metabolism
MH  - Th1 Cells/cytology/metabolism
MH  - Th17 Cells/cytology/metabolism
MH  - Transforming Growth Factor beta/metabolism
OTO - NOTNLM
OT  - Crohn's disease
OT  - IL10
OT  - IL23
OT  - Macrophages
OT  - TGF-beta
EDAT- 2020/06/20 06:00
MHDA- 2021/02/02 06:00
CRDT- 2020/06/20 06:00
PHST- 2020/01/17 00:00 [received]
PHST- 2020/05/05 00:00 [revised]
PHST- 2020/06/17 00:00 [accepted]
PHST- 2020/06/20 06:00 [pubmed]
PHST- 2021/02/02 06:00 [medline]
PHST- 2020/06/20 06:00 [entrez]
AID - 10.1002/eji.202048555 [doi]
PST - ppublish
SO  - Eur J Immunol. 2020 Nov;50(11):1676-1690. doi: 10.1002/eji.202048555. Epub 2020 
      Jun 29.