PMID- 32557772
OWN - NLM
STAT- MEDLINE
DCOM- 20210917
LR  - 20210917
IS  - 1097-4547 (Electronic)
IS  - 0360-4012 (Linking)
VI  - 98
IP  - 10
DP  - 2020 Oct
TI  - Direct arterial damage and neurovascular unit disruption by mechanical 
      thrombectomy in a rat stroke model.
PG  - 2018-2026
LID - 10.1002/jnr.24671 [doi]
AB  - Mechanical thrombectomy (MT) is a standard treatment for acute ischemic stroke 
      that could cause hemorrhagic complications. We aimed to evaluate the pathology of 
      MT-induced arterial damage and neurovascular unit (NVU) disruption in relation to 
      tissue-type plasminogen activator (tPA) injection for acute ischemic stroke. We 
      induced transient middle cerebral artery occlusion in male SHR/Izm rats for 2 hr. 
      This was followed by reperfusion with/without tPA (3 mg/kg) and "rough suture" 
      insertion that mimicked MT once or thrice (MT1 or MT3). Compared with the control 
      group, the tPA + MT3 group presented with an increase in the cerebral infarct and 
      hemorrhage with severer IgG leakage. Moreover, structural damage reaching the 
      tunica media was detected in the MT3 and tPA + MT3 groups. The tPA + MT3 group 
      presented with increased matrix metalloproteinase-9 (MMP-9) and vascular 
      endothelial growth factor (VEGF) expression with some MMP9-positive cells 
      expressing a neutrophil marker myeloperoxidase. Furthermore, basal lamina 
      detachment from astrocyte foot processes was observed in the tPA + MT1 and 
      tPA + MT3 groups. These findings suggest that MT causes direct arterial damage, 
      as well as VEGF and MMP9 upregulation, which results in NVU disruption and 
      hemorrhagic complications in acute ischemic stroke, especially when combined with 
      tPA.
CI  - (c) 2020 Wiley Periodicals, Inc.
FAU - Sasaki, Ryo
AU  - Sasaki R
AD  - Department of Neurology, Graduate School of Medicine, Dentistry and 
      Pharmaceutical Sciences, Okayama University, Okayama, Japan.
FAU - Yamashita, Toru
AU  - Yamashita T
AD  - Department of Neurology, Graduate School of Medicine, Dentistry and 
      Pharmaceutical Sciences, Okayama University, Okayama, Japan.
FAU - Tadokoro, Koh
AU  - Tadokoro K
AUID- ORCID: 0000-0002-5049-0875
AD  - Department of Neurology, Graduate School of Medicine, Dentistry and 
      Pharmaceutical Sciences, Okayama University, Okayama, Japan.
FAU - Matsumoto, Namiko
AU  - Matsumoto N
AD  - Department of Neurology, Graduate School of Medicine, Dentistry and 
      Pharmaceutical Sciences, Okayama University, Okayama, Japan.
FAU - Nomura, Emi
AU  - Nomura E
AD  - Department of Neurology, Graduate School of Medicine, Dentistry and 
      Pharmaceutical Sciences, Okayama University, Okayama, Japan.
FAU - Omote, Yoshio
AU  - Omote Y
AD  - Department of Neurology, Graduate School of Medicine, Dentistry and 
      Pharmaceutical Sciences, Okayama University, Okayama, Japan.
FAU - Takemoto, Mami
AU  - Takemoto M
AD  - Department of Neurology, Graduate School of Medicine, Dentistry and 
      Pharmaceutical Sciences, Okayama University, Okayama, Japan.
FAU - Hishikawa, Nozomi
AU  - Hishikawa N
AD  - Department of Neurology, Graduate School of Medicine, Dentistry and 
      Pharmaceutical Sciences, Okayama University, Okayama, Japan.
FAU - Ohta, Yasuyuki
AU  - Ohta Y
AD  - Department of Neurology, Graduate School of Medicine, Dentistry and 
      Pharmaceutical Sciences, Okayama University, Okayama, Japan.
FAU - Abe, Koji
AU  - Abe K
AUID- ORCID: 0000-0003-1563-7226
AD  - Department of Neurology, Graduate School of Medicine, Dentistry and 
      Pharmaceutical Sciences, Okayama University, Okayama, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200618
PL  - United States
TA  - J Neurosci Res
JT  - Journal of neuroscience research
JID - 7600111
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - 0 (vascular endothelial growth factor A, rat)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
RN  - EC 3.4.24.35 (Mmp9 protein, rat)
SB  - IM
MH  - Animals
MH  - Brain Ischemia/*etiology/metabolism/*pathology
MH  - Disease Models, Animal
MH  - Male
MH  - Matrix Metalloproteinase 9/metabolism
MH  - Neurovascular Coupling/*physiology
MH  - Rats
MH  - Rats, Inbred SHR
MH  - Stroke/*etiology/metabolism/*pathology
MH  - Thrombectomy/*adverse effects
MH  - Vascular Endothelial Growth Factor A/metabolism
OTO - NOTNLM
OT  - RRID:AB_2146325
OT  - RRID:AB_2212642
OT  - RRID:AB_2827429
OT  - RRID:AB_776512
OT  - RRID:AB_94844
OT  - hemorrhagic complication
OT  - ischemic stroke
OT  - mechanical thrombectomy
OT  - neurovascular unit
OT  - tPA
EDAT- 2020/06/20 06:00
MHDA- 2021/09/18 06:00
CRDT- 2020/06/20 06:00
PHST- 2020/02/12 00:00 [received]
PHST- 2020/04/11 00:00 [revised]
PHST- 2020/05/19 00:00 [accepted]
PHST- 2020/06/20 06:00 [pubmed]
PHST- 2021/09/18 06:00 [medline]
PHST- 2020/06/20 06:00 [entrez]
AID - 10.1002/jnr.24671 [doi]
PST - ppublish
SO  - J Neurosci Res. 2020 Oct;98(10):2018-2026. doi: 10.1002/jnr.24671. Epub 2020 Jun 
      18.